| Project/Area Number |
06680580
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Tokyo Institute of Thechnology |
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Principal Investigator |
FUKUMORI Yoshihiro Tokyo Inst.Techn.Life Sci.Associate Professor, 生命理工学部, 助教授 (60135655)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | magnetic bacterium / magnetic particle / localized pretein / TPR motif / protein import receptor / protein-protein interaction / MAM22 / マグネタイト / 蛋白質 / 細菌 / 局在 |
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| Research Abstract |
I cloned and sequenced the gene encoding MAM22 which was localized in magnetosome membranes of Magnetospirillum magnetotacticum. The amino acid sequence was deduced to be MSSKPSDILD-EVTLYAHYGLSVAKKLGMNMVDAFRAAFSVNDDIRQVYYRDKGISHAKAGRYSQAVMLLEQVYDADAFDVDVALHLGIAYVKTGAVDRGTELLERSLADAPDNVKVATVLGLTYVQVQKYDLAVPLLIKVAEANPINFNVRFRLGVALDNLGRFDEAIDSFKIALACVPMKARCIAPSPSAMSRWAGTRKPCRISRRPMNLTKGPRSEAV (DDBJ Accession No.D82942). The deduced amino acid sequence of MAM22 was compared with the Gen Bank and SWISS PROT data base by the BLAST program. It was homologous to a number of proteins that belong to TPR protein family. MAM22 contained three tandemly arranged TPR units in its center. The proteins with TPR motifs have been identified in the peroxisomal protein import receptors and the mitochondrial outer membrane proteins which are thought to function as import receptors for nuclear encoded preproteins. The finding of a protein with TPR motifs in magnetosomes would provide important suggestions concerning the mechanism of magnetite synthesis in magnetotactic bacteria.
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