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FUNCTION ANALYSIS OF COATOMER IN VESICLE TRANSPORT

Research Project

Project/Area Number 06680596
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionSASAKI INSTITUTE

Principal Investigator

KUGE Sayuri  SASAKI INSTITUTE BIOCHEMISTRY RESEARCHER, 生化学部, 研究員 (50260104)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsVesicle Transport / Coatomer / epsilon-COP / coatmer / _ε-COP / ζ-COP
Research Abstract

The cDNA encoding epsilon-COP,the 36-kD subunit of coatomer, was cloned form a bovine liver cDNA library and sequenced. Immunoblotting with an anti-epsilon-COP antibody showed that epsilon-COP exists in COP-coated vesicles as well as in the cytosolic coatomer. Using the cloned cDNA,recombinant His_6-tagged epsilon-COP was overexpressed in cultured Chinese hamster ovary (CHO) cells, from which metabolically radiolabeled coatomer was purified by taking advantage of the His_6 tag. Radiolabeled coatomer was employed to establish that all the subunits of the enter coated vesicles as an intact unit.
cDNA encoding the 20-kD subunit of coatomer, zeta-COP,predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to zeta-COP blocks the binding of coatomer to Golgi membranes and prevents the assembly of COP-coated vesicles on Golgi cisternae. Unlike other coatomer subunits (beta-, beta'-, gamma-, and epsilon-COP), zeta-COP exists in both coatomer bound and free pools.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 久下理: "ζ-COP,a subunit of coatomer,is required for COP-coated vesicle assembly." Journal of Cell Biology. 123. 1727-1734 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 久下小百合: "En bloc Incorporation of coatomer subunits during the assembly of COP-coated vesicles." Journal of Cell Biology. 124. 883-892 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 久下理: "細胞内蛋白質輸送に関与するCOP被覆小胞の形成機構と機能" 蛋白質、核酸、酵素. 40. 2427-2435 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Hara-Kuge, O.Kuge, L.Orci, M.Amherdt, M.Ravazzola, F.T.Wieland, and J.E.Rothman.: "En bloc Incorporation of coatomer subunits during the assembly of COP-coated vesicles." J.Cell Biol.124. 883-892 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] O.Kuge, S.Hara-Kuge, L.Orci, M.Amherdt, F.T.Wieland and J.E.Rothman: "zeta-COP,a subunit of coatomer, is required for COP-coated vesicle assembly." J.Cell Biol.123. 1727-1734 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] O.Kuge, S.and Hara-Kuge: "COP-coated vesicles in intracellular protein transport" Protein, Nucelic acid and Enzyme. 40. 2427-2435 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 久下 理: "ζ-COP,a Subunit of Coatmer, Is Required for COP-Coated Vesicle Assembly" Journal of Cell Biology. 123. 1727-1734 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] 久下 小百合: "En bloc Incorporation of Coatmer Subunits During the Assembly of COP-Coated Vesicles" Journal of Cell Biology. 124. 883-892 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 久下 理: "細胞内蛋白質輸送に関与するCOP被覆小胞の形成機構と機能" 蛋白質・核酸・酵素. 40. 2427-2435 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 久下 理: "ζ-COP,a Subunit of Coatomer,Is Required for COP-Coated Vesicle Assembly" Journal of Cell Biology. 123. 1727-1734 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 久下小百合: "En bloc Incorporation of Coatomer Subunits During the Assembly of COP-Coated Vesicles" Journal of Cell Biology. 124. 883-892 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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