• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on Processing Endoproteases of Biologically Active Peptide Precursors

Research Project

Project/Area Number 06680604
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionUniversity of Tsukuba

Principal Investigator

NAKAYAMA Kazuhisa  Univ.of Tsukuba, Inst.of Biological Science Associate Prof., 生物科学系, 助教授 (40192679)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsProcessing endoprotease / Kex2 protease / furin / paired basic amino acids / Kex2プロテアーゼ / Kex2 プロテアーゼ / プロホルモン
Research Abstract

In endocrine and neuronal cells, many biologically active peptides are synthesized as larger precursors, which undergo limited endoproteolysis at paired basic amino acids to yield bioactive products. Recently, six endoproteases (furin, PC2, PC3, PC4, PACE4, and PC6) has been shown to be involved in this type of processing. In this study, we have shown the followings with respect to furin.
1. We have discovered a mammalian cell line that lacks endoproteolytic activity due to mutations of furin. We have shown using this cell line that furin is the most important protease for processing of precursors of many proteins.
2. Furin is mainly localized to the trans-Golgi network (TGN) within cells. We have shown that, for the TGN-localization of furin, its cytoplasmic domain is essential, and Ser and Tyr residues within this domain play an important role.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Senye Takahashi: "A second mutant allele of furin in the processing-incompetent cell line. LoVo." J. Biol. Chem.270. 26565-26569 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Senye Takahashi: "Localization of furin to the trans-Golgi network and recycling from the cell surface involves Ser and Tyr residues within the cytoplasmic domain." J. Biol. Chem.270. 28397-28401 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Senye Takahashi: "A second mutant allele of furin in the processing-incompetent cell line, LoVo" J.Biol.Chem.270. 26565-26569 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Senye Takahashi: "Localization of furin to the trans-Golgi network and recycling from the cell surface involves Ser and Tyr residues within the cytoplasmic domain" J.Biol.Chem.270. 28397-28401 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takahashi, S.: "A second mutant allele of furin in the processing-incompetent cell line, LoVo." J. Biol. Chem.270. 26565-26569 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takahashi, S.: "Localization of furin to the trans-Golgi network and recycling from the cell surface involves Ser and Tyr residues within the" J. Biol. Chem.270. 28397-28401 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Brennan,S.O.: "Furin has the proalbumin substrate specificity and serpin inhibitory properties of an in situ hepatic convertase." FEBS Lett.338. 147-151 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohnishi,Y.: "A furin-defective cell line is able to process correctly the gp160 of human immunodeficiency virus typel." J.Virol.68. 4075-4079 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takahashi,S.: "Sequence requirements for endoproteolytic processing of precursor proteins by furin." J.Biochem.116. 47-52 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Brennan,S.O.: "Cleavage of proalbumin peptides by furin reveals unexpected restrictions at the P_2 and P'_1 sites." FEBS Lett.347. 80-84 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hiromoto,T.: "Both.proprotein-processing endoproteases,PC6 and furin,activate hemagglutinin of virulent influenza viruses." J.Virol.68. 6074-6078 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hosaka,M.: "PACE4A is a ubiquitous endoprotease that has similar but not identical substrate specificity to other Kex2-like processing endoproteases." Biomed.Res.15. 383-390 (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi