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Molecular cloning and functional characterization of hSNF2

Research Project

Project/Area Number 06680606
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

KATO Hiroyuki  Hokkaido Univ., Fac.of Pharmaceutical Sci.Assoc Pro., 薬学部, 助教授 (60185866)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
KeywordsSNF / chromatin / transcription / interaction / SNF2 / 転写活性化 / 核内リセプター / 転写因子 / 核内受容体
Research Abstract

A set of genes (SWI1, SWI2/SNF2, SWI3, SNF5 and SNF6) of Saccharomyces cerevisiae are required for transcription of a variety of yeast genes. It was reported that the mammalian glucocorticoid receptor failed to activate transcription in swi1^-, swi2^- or swi3^- strains. We report here that two mutually highly related human cDNAs (hSNF2alpha and -beta) encode amino acid sequences homologous to the yeast SWI2/SNF2 and the Drosophila brahma. Both of these contain the helicase motifs, a bromodomain, highly charged C-terminal sequences and N-terminal sequences rich in proline, glutamine and glycine. Tissue distribution of the mRNAs was somewhat different. Transcription activated by the estrogen receptor and the retinoic acid receptors was differentially enhanced by the expression of these cDNAs, although no enhancement was observed for several promoters which do not respond to nuclear receptors. We suggest that global transcriptional activators, or global coactivators, equivalent to the yeast SWI/SNF complex exist in mammalian cells.
The SNF/SWI complex facilitates efficient expression of a specific set of genes in yeast through remodelling chromatin structure. The human SNF/SWI complexes appear to share analogous subunit composition and function. The components of the human SNF/SWI complex, hSNF2a/b, specifically interact with TMF which was previously identified as a DNA binding protein to a TATA-like sequence. TMF is co-fractionated with the SNF/SWI complexes in a human cell nuclear extract. TMF localizes in nucleus and Golgi apparatus. Ectopic expression of TMF leads to down-regulation of retinoic acid receptor-dependent and SNF-enhanced gene expression.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Chiba,H.: "Two human homologues of Saccharomyces cerevisiae SNF2 and Drosophila brahma are with the estrogen receptor and the retinoic acid receptor." Nucleic Acids Res.22. 1815-1820 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Pognonec,P.: "Cross-family interaction between the bHLHZip USF and bZip Fral proteins results in down-regulation of AP1 activity" Oncogene. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Chiba, H.: "Two human homologues of Saccharomyces cerevisiae SNF2 and Drosophila brahma are transcriptional coacctivators cooperating with the estrogen receptor and the retinoic acid receptor." Nucleic Acids Res.22. 1815-1820 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Pognonec, P.: "Cross-family interaction between the bHLHZip USF and bZip Fral proteins results in down-regulation of AP1 activity." Oncogene. (in press.). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 千葉 晴美: " Two human homologues of Saccharomyces cerevisiae SW12/SNF2 and Drosophila brahma are transcriptional coactivators cooperaring with the estrogen receptor and the retinoic acid receptor" Nucleic Acids Research. 22. 1815-1820 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 千葉晴美: "Two human homologues of Saccharomyses cerevisiae SW12/SNF2 and Drosophila brahma are transcriptional coactivators cooperaring with the estrogen receptor and the retinoic acid receptor" Nuclcic Acids Rcsearch. 22. 1815-1820 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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