| Project/Area Number |
06680627
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Soka University |
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Principal Investigator |
AOYAMA Yuri Soka Univ., Fac.Engineering Lecturer, 工学部, 講師 (00158718)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Naohiro Soka Univ., Fac.Engineering Assistant Professor, 工学部, 助教授 (50214888)
HORIUCHI Tadao Soka Univ., Fac.Engineering Professor, 工学部, 教授 (10037567)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Brain / P450 / Cholesterol biosynthesis / Lanosterol demethylation / P450_<14DM> |
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| Research Abstract |
In order to obtain a clue to estimate whether brain cholesterol is synthesized in situ or not, occurrence of a P450 enzyme catalyzing lanosterol 14-demethylation (P450_<14DM>) was examined. Incubation of lanosterol with rat brain microsomes under the presence of NADPH and molecular oxygen formed the known 14-demethylated metabolite. This metabolism was inhibited by ketoconazole, a potent P450_<14DM> inhibitor as well as CO.These facts strongly indicated the occurrence of P450_<14DM> in rat brain microsomes. The brain demethylase activity was altered a little during maturation. In situ hybridyzation experiment with rat P450_<14DM> cDNA revealed that P450_<14DM> mRNA was expressed at the limited site of the brain. Lines of evidence obtained in this study suggested the occurrence of de novo cholesterol biosynthesis in brain.
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