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Observation of Biological Specimens by a Zone-Plate X-Ray Microscope using a Laser Plasma Source

Research Project

Project/Area Number 06680652
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionOsaka University

Principal Investigator

ARITOME Hiroaki  OSAKA UNIVERSITY,RESEARCH CENTER FOR EXTREME MATERIALS ASSOCIATE PROFESSOR, 極限物質研究センター, 助教授 (70029552)

Co-Investigator(Kenkyū-buntansha) YANAGIDA Toshio  OSAKA UNIVERSITY FACULTY OF ENGINEERING SCIENCE PROFESSOR, 基礎工学部, 教授 (30089883)
SHINOHARA Kunio  THE UNIVERSITY OF TOKYO,FACULTY OF MEDICINE PROFESSOR, 医学部, 教授 (10112088)
WAKAYA Fujio  OSAKA UNIVERSITY,FACULTY OF ENGINEERING SCIENCE RESEARCH ASSISTANT, 基礎工学部, 助手 (60240454)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsX-ray / Microscope / X-ray optics
Research Abstract

Various specimens are observed by a zone-plate x-ray microscope using x-rays (wavelength 3.37 nm) from a cabon plasma generated by a moving slab glass laser (5 J,3 Hz) and a backside illuminated CCD.
In the case of a metal mesh, good contrast was obtained by 5 x-ray pulses for the magnification of 100. For the sample of a zone plate 100 pulses were necessary for the magnification of 100. The resolution of 0.5 um was obtained.
As a biological spcecimen, dryed Hela cells were observed. By 20 x-ray pulses, good contrast was obtained for the magnification of 100. Microvillus of about 0.5 um width could be observed.
By improving x-ray optics, the improvement of resolution can be expected.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Shigeru Nakayam: "Zone plate X-ray microscope using a laser plasmasorce" Jpn. J. Appl. Phys.33. L1280-L1282 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Guan-ming Zeng: "Soft x-ray spectra of highly ionized elements with atomic numbers ranging from 57 to 82 produced by compact lasers" J. Appl. Phys.75. 1923-1930 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroyuki Daido, et al.: "Subnanosecond in-line soft x-ray holography using germanium laser in the 20nm wavelength region" J. X-Ray Science and Technology. 5. 105-120 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kunio Shinohara, et al.: "X-ray holographic microscopy of biological specimens" J. Synchrotron Radiation. 3. 35-40 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A. Ito, et al.: "Measurement of soft x-ray absorption spectra and elemental analysis in local regions of mammalian cells using an electronic zooming tobe" J. Microscopy. 181. 54-60 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shigeru Nakayama: "Zone-plate x-ray microscope using a laser plasma source" Jpn.J.Appl.Phys.33. L1280-L1282 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Guan-ming Zeng: "Soft x-ray spectra of highly ionized elements with atomic numbers raging from 57 to 82 produced by compact lasers" J.Appl.Phys.75. 1923-1930 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroyuki Daido: "Subnanosecond in-line soft x-ray holography using germanium laser in 20 nm wavelenght region" J.X-Rsy Science and Technology. 5. 105-120 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kunio Shinohara: "X-ray holographic microscopy of biological spcimens" J.Synchrotron Radiation. 3. 35-40 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Ito: "Measurement of soft x-ray absorption spectra and elemental analysis in local regions of mammalian cells using an electronic zooming tube" J.Microscopy. 181. 54-60 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Daido et al.: "Sobnano-second in-line soft X-ray holography using germanuom taser in the 20nm wave length region" J.X-ray Science and Technology. 5. 105-120 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Shinobara et al.: "X-ray holograbhic microscopy of biological specimens" J.Synchrotron Raiation. 3. 35-40 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Ito et al.: "Measorement of soft X-ray absorption spectra and elemental alalysis in local regions of mamimalian cells using an electronic zooingitobe" J.Microscopy. 181. 54-60 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Nakayama et al.: "Zone-plate x-ray microscope using a laser plasma source" Jpn.J.Appl.Phys.33. L1280-L1282 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] G.M.Zeng et al.: "Soft x-ray spectra of highly ionized elements with atomic numbers ranging from 57 to 82 produced by compact lasers" J.Appl.Phys.75. 1923-1930 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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