Mechanism of epldermal mucous melaplasia induced by rellnoid in cullured chich embryonic skin
| Project/Area Number |
06680697
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Teikyo University |
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Principal Investigator |
OBINATA Akiko Teikyo University.assistant professor, 薬学部, 助教授 (80112762)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Retinoid / Epidermis / Dermal fibroblast / Mucous Metaplasia / Skin / Tunikamycin / Differential Display / 上皮-間充織相互作用 |
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| Research Abstract |
When small pieces of epidermis or skin of chick embryo were cultured on retionol (20 muM) -pretreated dermal fibroblasts, epidermal mucous metaplasia was induced, suggesting that an interaction of epidermal keratinocyte with retinol-pretreated dermal fibroblast was essential for the transdifferentiation of the keratinocyle. Treatment of the skin with tunicamycin or pnitrophenyl-beta-D-xyloside did not inhibit retinol-induced mucous metaplasia, indicating that N-linked protein glycosylation and formation of proteoglycan are not required for this epithelial-mesencymal interaction. Monoclonal antibody prepared against the retinol-treated fibroblast prevented retinol-induced mucous metaplasia and immunohistochemical study indicated localization of the antibody in outer surface of the cell membrane. Some cDNA clones, whose expression were shown to be limiteed to retinol-treated fibroblasts by Northern hybridization analysis, were obtained by differential display between cDNAs of retinol-treated and nontreated fibroblasts. Further studies are in progress to clone the cDNA with cDNA library prepard from retinol-treated fibroblast.
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Report
(4 results)
Research Products
(11 results)
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[Publications] Obinata, A., Akimoto, T., Kawamata, T., Shimizu, S., et al: "Inhibition by tunicamycin of mucin synthesis, not morphological changes, in epidermis during retinol-induced mucous metaplasia of chick embryonic cultured skin." Anat.Rec. 245. 715-723 (1996)
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