| Project/Area Number |
06680716
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
FUKAMACHI Hiroshi The University of Tokyo Graduate School of Science Instructor, 大学院・理学系研究科, 助手 (70134450)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Epithelial-Mesenchymal Interaction / Hepatocyte Growth Factor / Keratinocyte Growth Factor / RT-PCR / Primary Culture / Stomach / Pepsinogen / Cathepsin E / 小腸 / HGF / 表皮成長因子 / KGF / 形態形成 / 上皮細胞 / 増殖因子 / 基質 / 分化 |
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| Research Abstract |
1.Glandular stomach epithelial cells expressed Cathepsin E but not pepsinogen when cultured in the absence of mesenchyme, but they expressed pepsinogen when cultured with mesenchyme. We thus concluded that the differentiation of glandular stomach epithelial cells is determined by the epithelial-mesenchymal interaction.
2.Hepatocyte growth factor (HGF) region-specifically stimulated proliferation of gastro-intestinal epithelial cells in primary culture. RT-PCR analysis showed that gastro-intestinal mesenchymes and epithelia express HGF and HGF receptors, respectively. We thus concluded that HGF is involved in the epithelial-mesenchymal interaction in gastro-intestinal organs.
3.Keratinocyte growth factor (KGF) also region-specifically stimulated proliferation of gastro-intestinal epithelial cells in primary culture. RT-PCR analysis showed that gastro-intestinal mesenchymes and epithelia express KGF and KGF receptors, respectively. We thus concluded that KGF is also involved in the epithelial-mesenchymal interaction in gastro-intestinal organs.
4.The role of HGF and KGF in the development of glandular stomach was investigated by quantitative RT-PCR.Genes for HGF and HGF receptor were expressed at the morphogenetic period, and those for KGF and KGF receptor after the morphogenetic period. Thus we concluded that these two growth factors play different roles in developing glandular stomachs.
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