| Project/Area Number |
06680730
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
HOSOYA Yasuhiko College of Medical Technology and Nursing, University of Tsukuba Professor, 医療技術短期大学部, 教授 (60100145)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Symapthetic preganglionic neurons / Cholera toxin subunit B / Oxytocin / Immunohistochemistry / Rat |
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| Research Abstract |
A combination of retrograde cell body labeling and immunohistochemistry was employed to elucidate how oxytocinergic fibers make contact with sympathetic preganglionic neurons (SPNs) in the rat spinal cord from T_1 to T_4. SPNs were labeled retrogradely using cholera toxin subunit B (CTb) or horseradish peroxidase conjugated CTb. Oxytocin immunoreactive (ir) fibers were found in the intermediate zone including the sympathetic preganglionic subnuclei. In the central autonomic nucleus and the intercalated nucleus, brown-stained oxytocin-ir varicosities or terminals were frequently observed to stud black-stained dendrites of SPNs. Electron microscopical observations showed that oxytocin-ir terminals form synapses with dendrites or soma of the sympathetic preganglionic neurons. The terminals contained numerous small clear round vesicles and a few large cored vesicles. These results clearly show that a large part of SPNs is innervated by oxytocin containing fibers. The origin of these fibers is discussed, and it is concluded that they are probably descending fibers from the paraventricular nucleus of the hypothalamus.
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