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The significance of phosphorylation of neurofilament protein

Research Project

Project/Area Number 06680738
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionOsaka University

Principal Investigator

GOTOW Takahiro  Osaka University Medical School, Lecturer, 医学部, 講師 (20135693)

Co-Investigator(Kenkyū-buntansha) TAKEDA Masatoshi  Osaka University Medical School, Professor, 医学部, 教授 (00179649)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
KeywordsNeurofilament / NF-H / phosphorylation / axon / node of Ranvier / crossbridge / myelin sheath / nonphosphorylation / ニューロフィラメントH蛋白質 / 微細管 / ミトコンドリア / MAP2 / ポーリン / 培養細胞 / 細胞内微量注入 / NF-H遺伝子 / 免疫細胞化学 / 急速凍結ディープエッチング / ランビエ絞輪 / 軸索終末 / サイクロスポリンA / 急速凍結ディープエッチ法
Research Abstract

In neurons, neurofilaments (NF) are relatively fewer in somata and dendrites but abundant in axons. In addition to this NFpolarity, the NF subunit protein, especially NF-H,is extremely phosphorylated in axons, and much less phosphorylated in somata and dendrites. NF-H is essential for forming crossbridges between core filaments, and we are thinking that its phosphorylation seems necessary to form well-developed crossbridges. To confirm our idea, we have obtained five main results on the relationship between NF-H phosphorylation and NF organization during these three years. 1).Both core filaments and crossbridges were regular in arrangement and NF-H was well phosphorylated in thicker myelinated axons, whereas they were less regular and less phosphorylated in thinner axons where microtubules were conspicuous. 2).In the node of Ranvier, where axon becomes smaller in diameter, core filametns were closer to each other and crossbridges were less regular and NF-H wa less phosphorylated than t … More he remainder of the same axon. 3).After microinjection of NF-H protein into cultured nonneuronal or neuron-like cells, NF-H was incorported into intermediate filaments and tended to make bundles of the filaments with rather higher frequency in crossbridges when it was phosphorylated. 4).In vitro experiments showed that NF could interact with microtubules or mitochondria through the crossbridges formed by NF-H,although we are now examining which condition in phosphorylation of NF-H is more efficient for such interactions. 5).At present, we are clarifying the relationship between the myelination and NF organization or NF-H phosphorylation with jimpy mutanat mice, where the myelinogenesis is distrupted in the central nervous system. It has just been found that nonphosphorylated form of NF-H increases drastically and core filaments are much more compact in the myelin-deficient axons than in controls. In conclusion, our data indicate that there is an intimate relationship between NF-H phosphorylation and NF organization, and also that it is necessary for the axon to have regular arrangements in crossbridges and finally in core filaments by NF-H phosphorylation. Furthermore, it is considered that the myelination increases in NF-H phosphorylation level resulting in normal NF oganization in axons. Less

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Gotow, T.: "Phosphorylation of neurofilament H subunit as related to arrangement of neurofilaments" Journal of Neuroscience Research. 37. 691-713 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gotow, T.: "Dephosphorylation of the largest neurofilament subunit protein influences the structure of crossbridges in reassemble neurofilaments" Journal of Cell Science. 107. 1949-1957 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gotow, T.: "The organization of neurofilaments accumulated in perikaryon following aluminum infoxication : relationship between structure and phosphorylation of neurofilaments" Neuroscience. 64. 553-569 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gotow, T.: "Dynamic structure and function of neurofilaments" Annals of Psychiatry. 5. 91-111 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeda, M.: "Neurofilament pathology in neurodegenerative processes of Alzheimer's disease" Annals of Psychiatry. 5. 113-126 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gotow, T.: "Preferential localization of annexin V to the axon terminal" Neuroscience. 75. 507-521 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gotow, T.and Tanaka, J.: "Phosphorylation of neurofilament H subunit as related to arrangement of neurofilaments" Journal of Neuroscience Research. 37. 691-713 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gotow, T., Tanaka, T., Nakamura, Y.and Takeda, M.: "Dephosphorylation of neurofilament H subunit protein influences the structure of crossbridges in reassembled neurofilaments" Journal of Cell Science. 107. 1949-1957 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gotow, T.and Tanaka, J.: "The organization of neurofilaments accumulated in perikaryon following aluminum intoxication : relationship between structure and phosphorylation of neurofilaments" Neuroscience. 64. 553-569 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gotow, T.: "Dyanamic structure and function of neurofilaments" Annals of Psychiatry. 5. 91-111 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeda, M., Gotow, T.and Nishimura, T.: "Neurofilament pathology in neurodegenerative processes of Alzheimer's disease" Annals of Psychiatry. 5. 113-126 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gotow, T., Sakata, M., Funakoshi, T.and Uchiyama, Y.: "Preferential localization of annexin V to the axon terminal" Neuroscience. 75. 507-521 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gotow,T.: "Preferential localization of annexin V to the axon terminal" Neuroscience. 75. 507-521 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takeda,M.et al.: "Neurojilament pathology of Alzheimer's disease : neurofilament accimulation in duced by Alzheimer buffy ceat." Res.Adv.Alzheimer's Disesge Related Disorders. 553-559 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Gotow,T.: "Dynamic stracture and function of neurofilaments" Ann.Dsychiat.5. 91-111 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takeda,M et al.: "Neurofilament pathology in neurodegenerative process of Alzheimer disease" Ann,Psychiat.5. 113-126 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 後藤隆洋: "ニューロフィラメントの構築とリン酸化との関係" 医学のあゆみ. 175. 736-737 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Gotow,T.,Tanaka,J.: "Phosphorylation of neurofilament Hsubunit as ralatel to arrangement of neurofilaments" Journal of Neuroscience Research. 37. 691-713 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Gotow,T.,Tanaka,T.,Nakamura,Y.,Takeda M.: "Dephosphorylation of the largest neurofilament subumit protein influences the structure of crossbridges in reassembled neurofilaments" Journal of Cell Science. 107. 1949-1957 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 後藤隆洋: "免疫細胞化学のための急速凍結ディープエッチ法" 細胞. 26. 516-522 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Gotow,T.,Tanaka,J.,Takeda,M.: "The orgainzalion of neurofilaments accumulated in perilcaryon following aluminum administration:Relationship between structure and phosphorylation of neurofilaments" Neuroscience. 64. 553-569 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takeda,M.,Tanimurai,S.,Kanayama,G.,Gotow.T.,Nishimura,T.: "Neurofilament pathology of Alzheimer's disease:Neurofilament accumulation induced by Alzheimer buffy coat." (in press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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