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Regulation of expression of cytoskeletal proteins and neuropaptides by estrogen reaptor

Research Project

Project/Area Number 06680743
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Nerve anatomy/Neuropathology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

YURI Kazunari  Kyoto Prefectural University of Medicine, Department of Anatomy and neurobiology, Assistant Professor, 医学部, 講師 (10220534)

Co-Investigator(Kenkyū-buntansha) MORITA Noriyuki  Dokkyo University School of Medicine, Department of Histology and Neurobiology P, 医学部, 助手 (50239662)
UEDA Shuichi  Dokkyo University School of Medicine, Department of Histology and Neurobiology P, 医学部, 教授 (60150570)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Keywordsestrogen / estrogen receptor / sexual dimorphic nucleres / hypothalamus / medial preoptic area / neuron / 性的二型 / 分散培養 / バリコシティー / 極性 / 細胞骨格 / タウ / MAP2 / ラット
Research Abstract

To investigate the function of estrogen and estrogen receptor (ER) in the formation of sexual dimorphic nuclei in the brain, in vivo and in vitro studies were performed using the hypothalamus of the rat. Hypothalami were removed from female rats embryos, dissociated, plated onto coverslips and investigated using immunocytochemistry. MAP2-and/or tau-immunoreactive processes were elongated significantly 3 days after estrogen treatment. ER immunoreactive neurons had longer processes than that of control neurons. Low density dissociated culture of hypothalamus was used for scanning electron microscopy. Although no difference was observed between estrogen treated and non treated neurons after the first 12 hours of culture, elongation of axon-like processes and formation of varicosities were recognized after 24 hours in estrogen treated neurons. Expression of ER in the embryonal stage of the male and female rats was investigated using immunocytochemistry. ER immunoreactive neurons were detected in embryonic day 17 in female and day 19 in male rat preoptic area. The intensity of immunoreactivity was stronger in female than in male. These results suggest that estrogen may affect hypothalamic neurons in embryonal stage throught ER : it may promote the elongation of the processes with establishment of polarity and formation of varicosities in the sexual dimorphic nuclei.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Yuri,K: "Estrogen affects calcitoniu geue-relates pepticle and meihioruine-enkyhalim-immunreactive vleupon in the female rat precptic area." Neuroscience Letters. 169. 5-8 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yuri,K: "Region-spefific changes of tycsine hydroxylase-immumo reactivity by estrogen troatment in female eat hypotholamus" Brain Research. 645. 278-284 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yuri,K: "Estrogen reccptor-immuncreactive neurons contain caicitonin gene-related peptide ,methionine-enkephilin or tyrisine hgdroxgiase in the female rat precptic area" Neuroscience Rescarch. 21. 135-141 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Pasterkamp,R.J.: "The perinatal cntogeny of estrogen receptor-immunoreactivty in the developing male and female rat hypothalamus" Developmental Brain Research. (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hu,Z.: "Exposure of postnatal rats to glucocorticoids suppresses the development of choline acetyltransferase-immunoreactive neurons: roleof adrena steroids in the development of forebrain Cholinerglc neurons" Journal of Chemical Neuroanatomy. 1996

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yang,Y: "The effects of nerve growth factor on the newborn rat dorsalroot ganglion neurons in vitro: a scanning electron micruscopic observation" Journal of Brain Research. (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 由利和也: "ニューロサイエンスラボマニュアル1神経細胞培養法" シュプリンガーフェアラーフ東京、畠中寛編集, (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yuri, K: "Estrogen affects calcitomin gene-related peptide-and methionine-enkephalin-immuno reactive neuron in the female rat preoptic area" Neuroscience Letters. 169. 5-8 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yuri, K: "Region-specific changes of tyrosine hydroxylase-immunoreactivity by estrogen treatment in female rat hypothalamus" Brain Research. 645. 278-284 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yuri, K: "Estrogen receptor-immunoreactive neurons contain calcitonin gene-related peptide, methionine-enkephalin or tyrosine hydroxylase in the female rat precptic area" Neuroscience Research. 21. 135-141 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Pasterkamp, R.J.: "The perinatal ontogeny of estrogen receptor-immunoreactivity in the develeping male and female rat hypothalamus" Developmental Brain Research. (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hu, Z: "Exposure of postnatal rats to glucocorticoids suppresses the development of choline acetyltransferase-immunoreactive neurons : role of adrenal steroids in the development of forebrain cholinergic neurons" Journal of Chemical Neuroanatomy. (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yang, Y.: "The effects of nerve growth faetor on the newborn rat dorsal root ganglion neurons in vitro : a scanning electron microscopic observation" Journal of Brain Research.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] R.Jeroen Pasterkamp: "The perinatal ontogeniy of estrogen receptor-immunoreactivity in the developirg male and female rat hrppo ihalamus" Develepinental Brain Reseonch. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hu,Z.: "Exposure of postnatal rats to glucocoids suppresses the development of choline acetyltrans ferase-immunoreactive neurons:role of adrenal steroids in the development of forehrain cholinergic neurons" J,Chemical Neuroanatomy. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yang,Y.: "The effects of nerve growth factor on the newborn rat dorsal root ganglion neurons in vitro:a scanning electron microscopic observation" J,Brain Research. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 由利和也: "ニューロンサイエンスラボマニュアル1 神経細胞培養法" シュプリンガーフェアラータ東京 畠中寛 編集, (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazunari Yuri: "Estrogen receptor-imminiceactive meurons contain calcitonin gene-related peptide,methionine-enkephalin or tyrosine hydroxylase in the female rat preaptic area." Neuroscience Research. 21. 135-141 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kazunari Yuri: "Region-specific changes of tyrosine hydroxylase-immunoreactivity by estrogy treatment in female rat hypothalamus" Brain Research. 645. 278-284 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kazunari Yuri: "Estrogen affects calitorin gene-related peptide-and methionine-enkophalin-immunoreactive heuron in the femile rat preoptl area." Neurascience Letters. 169. 5-8 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 河田 光博: "ホルモンの標的組織としての視床下部" 内分泌学の進歩. 11. 44-51 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Mitsuhiro Kawata: "Steroid hormone effects on gene expression,neurnal stsucture,and defferentiation" Hormones and Behavior. 28. 477-482 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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