| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
Since the somatic recombination of myelin basic protein (MBP) had been suggested by our precious works, we have performed a line of experiments to prove it. We performed LA-PCR,but no amplified band was observed, suggesting that the distance between unidentified exon 0 and Golli-mbp exon 5a might be much longer than we expected. We also carried out genomic Southern using the TRT probe, which is a 298 bp fragment of 5'-end of M41-MBP cDNA and is composed of a part of exon 0 and part of Golli-mbp exon 5a (Kitamura, K., et al., J.Neurochem., 54.2032(1990)). However, any significant differences were not observed between brain and liver DNAs from C57BL/6J and ddY mouse Since a possibility that the recombination event might be occurred only in extremely small number of cells in the brain could not be ruled out, we used large amount of DNA samples and highly labeled TRT probe for genomic Southern. blot. We could observe a difference between brain and liver genomic DNAs on the blot. However, the signals were not strong enough to prove the recombination of MBP gene in the brain. We have to develop some new techniques to detect convincingly such weak signals as from rare recombination, before we reach the conclusion.
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