| Project/Area Number |
06680778
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
SAITO Yumiko Tokyo Metropol Inst Med Sci., Dept Mol Biol., Resercher, 遺伝子情報研究部門, 研究員 (00215568)
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| Co-Investigator(Kenkyū-buntansha) |
MARUYAMA Kei National Inst Physiol Sci., Lab Neurochem., Associate Professor, 生理研・神経化学, 助教授 (30211577)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Neural differentiation / Gene / Splicing / Subtraction / Brain / Neuropeptide |
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| Research Abstract |
We used a subtraction cloning approach to isolate cDNAs of N23K and N27K, whose mRNA and protein are transiently up-regulated in mouse NS20Y cells undergoing neurite outgrowth induced by treatment of dibutyryl cyclic AMP.N23K and N27K encode precursor proteins for a newly discovered rat neuropeptide nociceptin. As with other neuropeptide precursors, the N23K/N27K proteins contain a putative signal peptide rich in hydrophobic amino acids at the N-terminus. N23K/N27K mRNA are present only in brain and spinal cord in adult mouse, and are expressed at higher levels in early postnatal brain. These findings suggest that the N23K/N27K protein function not only as neuropeptide precursors but also as important components in neuronal differentiation.
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