| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Research Abstract |
Chediak-Higashi syndrome (CHS) is an autosomal recessive genetic disease that has been reported in human, mink, cats, and mice. CHS was initially recognized as a disorder in which melanocytes, neutrophils, and lymphocytes contained giant cytoplasmic granules. The mutation of mice comparable to human CHS is named "beige " from the diluted coat color. If CHS rats are available, they are probably useful for investigations such as biochemical analysisof CHS.We found that the coat color mutant DA strain maintained in Hamamatsu University School of Medicine was comparable to human CHS and it was named DA-bg. In this research we attempted to mapping bg gene on rat chromosome. The following results were obtained,
The mouse Chr.13 is comparable to the rat Chr.17 so, we collected 8 of microsatellite marker (MSM). The 6 of MSM were amplified by PCR method. The 10 of inbred strains of rats were tested by these MSM and it was found polymorphism between DA-bg and BN at two (Prolactin : PRL and Cholinergic recepter, muscaric3 : ACRM) of MSM.In 80 progeny of {(DA-bg*BN)*DA-bg} backcross, it was found linkage between PRL to bg (30cM), ACRM to bg (23cM), and PRL to ACRM (11 cM). It was confirmed that the bg gene was on Chr.17 in rat.
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