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In vivo experimeted model for anticancer therapy using murine and humanized monoclonal antibodies

Research Project

Project/Area Number 06680838
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionTokai University School of Medicine

Principal Investigator

SHIMAMURA Kazuo  Tokai University, School of Medicine, Accistant Pro., 医学部, 講師 (00119679)

Co-Investigator(Kenkyū-buntansha) TOKUDA Yutaka  Tokai University, School of Medicine, Accistant Pro., 医学部, 講師 (20163975)
TAMAOKI Norikazu  Tokai University, School of Medicine, Pro., 医学部, 教授 (50055860)
UEYAMA Yoshito  Tokai University, School of Medicine, Assciate Pro., 医学部, 助教授 (30072408)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Keywordsc-erbB-2 / SCID mice / anti-tumor effect / humanized monoclonal antibody / SCID mouse / human gastric carcinoma / monoclonal antibody / immunotherapy
Research Abstract

The c-erbB-2 product is thought to be an unique and useful target for antibody therapy of cancers overexpressing the c-erbB-2 gene. In vitro and in vivo antitumor effects of a humanized antibody against the extracellular domain of the c-erbB-2 gene product, rhu4D5, were examined. Rhu4D5 was less effective than its murine counterpart, mu4D5, for the direct antiproliferative activity against the c-erbB-2 overexpressing SK-BR-3 cell line. In vivo treatment of SCID mice carrying the c-erbB-2 overexpressing 4-1ST human gastric carcinoma xanograft anti-tumor activity. Furthemore, cytotoxicity of human peripheral blood mononuclear cells against 4-1ST was signnificantly augmented with rhu4D5, but not with mu4D5.
These results indicate that rhu4D5 might be better in patients than predicted from preclinical studies.
Injection of 4-1ST cells into the tail vein resulted in death of the mice within 80 days. Threatment of animals with rhu4D5 prolonged murine survival time slightly but not the level with mu4D5.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Ohishi Y.,Shimamura K.,et al: "Prolonged survival of mice with human qastric cancer treated with an anti-c-erbB-2 monoclonal antibody" Br.J.Cancer. 71. 969-973 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tokuda Y.,Shimamura K.,et al: "In vivo and in vitro antitumor effect of a humanized monoclonal antibody against c-erbB-2 product" Br.J.Cancer. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ohnishi, Y., Shimanura, K., et al.: "Prolonged survival of mice with human gastric cancer treated with an anti-c-erbB-2 monoclonal antibody" Br.J.Cancer. 71. 969-973 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tokuda, Y., Shimamura, K., et al.: "In vivo and invitro antitumor effect of a humanized monoclonal antibody a against c-erbB-2 product" Br.J.Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ohishi, Y. Shimamura, K, et al: "Prolonged survival of mice with human gastric cancer treated with an antic-erbb-2 monoclonal antibody" Br. J. Cancer. 71. 969-973 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tokuda, Y. Shimamura, K, et al: "In vivo and in vitoro antitumor effect of a humanized monoclonal antibody against c-erbB-2 product" Br. J. Cancer. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Yasuyuki,Ohnishi,Kazuo,Shimamura,etal.: "“Prolonged survival of mice with human gastric cancer treated with an anti-c-erbB-2 monoclonal antibody"" British Jourrnal of Cancer. 71(in press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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