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In Vitro Studies on Human Endothelial Cells, Seeded on Materials for the purpose of Artificial Blood Vessels

Research Project

Project/Area Number 06680853
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SATO Hiroko  Kyoto University, Graduate School of Engineering, Department of Polymer Chemistry, Instructor, 工学研究科, 助手 (00093245)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordshuman vascular endothelial cells, HUVEC / artificial blood vessel / polymer materials / anti thrombogenicity / procoagulant / extracellular matrices / tissue plasminogen activator, tPA / plasminogen activator inhibitor type 1, PAI-1 / tPA疎外タンパク質(PAH) / ガス滅菌 / 細胞毒性 / 蛍光合成基質 / 血栓溶解系酵素
Research Abstract

Vascular endothelial cells contact with blood in vivo form no thrombi in the normal state. Artificial polymer materials where human vascular endothelial cells (HUVEC) are seeded may be expected to lead to antithrombogenic surfaces. Such hybrid materials will be useful as artificial blood vessels for a long-term implantation. Vascular endothelial cells, however, stimulated with cytokines, are known to change to the procoagulant state.
In this study, the compatibility of HUVEC with artificial materials was investigated on fibrinolysis-related proteins, i.e., tissue-type plasminogen activator (tPA) and plasminogen inhibitor type 1 (PAI-1). The following results were obtained ;
1) HUVEC were observed to be released relatively high amount of tPA in the initial culture period for four days.
2) The activity of tPA,measured with a fluorogenic synthetic substrate, was scarcely detected in the culture medium of HUVEC,while the activity of tPA was detected in the HUVEC lysate component in which PAI-1 was inactivated. Probably, tPA,released from HUVEC,should form complex with PAI-1 in the culture medium.
3) The ratio of amount of tPA against that of PAI-1, released in the culture medium, was dependent on extracellular matrices (ECM), i.e., ECM proteins and artificial materials used.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 佐藤弘子: "人工材料に対するヒト血管内皮細胞の接着および増殖に関する研究(I)" 生体材料. 13. 105-111 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐藤弘子,森和哉: "人工材料に接着したヒト血管内皮細胞の増殖および細胞状態に関する研究(II)" 生体材料. 13. 160-166 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐藤弘子,森和哉: "血管内皮細胞の抗血栓性人工材料に対する適合性" 日本血栓止血学会誌. 6. 272-278 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroko Sato: "Selective adsorption of fibrinogen domains at artificial surfaces and its effect on endothelial cell spreading" Proteins at Interfaces, ACS Series. 602. 478-488 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroko Sato, Kozo Ono, and Masanori Oka: "Drug release through fibrinolysis of antibiotic-bound fibrin" Controlled Release. 29. 125-132 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroko Sato: "A study on the initial attachment and proliferation of human vascular endothelial cells, seeded on artificial surfaces (I)" Seitai-Zairyou (Biomaterials). 13-3. 105-111 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroko Sato and Kazuya Mori: "A study on proliferation and cell states of human vascular endothelial cells, attached on artificial surfaces (II)" Seitai-Zairyou (Biomaterials). 13-4. 160-166 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroko Sato and Kazuya Mori: "Compatibility of vascular endothelial cells with a thromboresistant artificial material" J.Japanese Soc.Thrombosis and Hemostasis. 6-4. 272-278 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroko Sato: "Selective adsorption of fibrinogen domains at art artificial surfaces and its effect on endothelial cell spreading" Proteins at Interfaces, ACS Sereies. 602. 478-488 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroko Sato, Kozo Ono, and Masanori Oka: "Drug release through fibrinolysis of antibiotic-bound fibrin" Controlled Release. 29-1-2. 125-132 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐藤弘子: "人工材料に対するヒト血管内皮細胞の接着および増殖に関する研究(I)" 生体材料. 13. 105-111 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐藤弘子: "人工材料に接着したヒト血管内皮細胞の増殖および細胞状態に関する研究(II)" 生体材料. 13. 160-166 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐藤弘子: "血管内皮細胞の抗血栓性人工材料に対する適合性" 日本血栓止血学会誌. 6. 272-278 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiroko Sato: "Selective adsorption of fibrinogen domains at artificial surfaces and its effect on endothelial cell spreading" Proteins at Interfaces, ACS Series. 602. 478-488 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiroko Sato: "Drug release through fibrinolysis of antibiotic-bound fibrin" Controlled Release. 29. 125-132 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐藤弘子: "人工材料に対するヒト血管内皮細胞の接着および増殖に関する研究" 生体材料,1995年2月受理. 13. (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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