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The function and role of telomeric palindrom sequence newly found in tobacco chromosomes

Research Project

Project/Area Number 06804046
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 遺伝
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

YOSHIDA Kazuo (1995)  Fac.Science, Biological Science, Professor, 理学部, 教授 (70022720)

鈴木 克周 (1994)  広島大学, 理学部, 助手 (50221320)

Co-Investigator(Kenkyū-buntansha) TOYOHARA Gentaro  Fac.Science, Biological Science, Lecturer, 理学部, 講師 (00033895)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordstelomere / RETS / chromosome repair / tobacco / yeasts / YAC (yeast artificial chromosome) / tandem repeats / trans-kingdom conjugation / 染色体末端 / テロメア- / テロメア付加 / 傷害 / 核DNA / タンデムリピート / 遺伝学
Research Abstract

The increasing DNA and linearlization of genomes (chromosome formation) are fundamental basis for divergence of eukaryotes from prokaryotes during evolution. The telomeres and telomerase are indispensable for eukaryotic linear chromosomes. Unfortunately, little is known the molecular structure of telomeres in plants. In this research project, we have found and sequenced novel RETS (restriction enzymes-resistant high molecular weight telomeric DNA sequences) in tabacco chromosomes. By using RETS as a probe, we characterized the terminal structure of tobacco chromosomal ends. Furthermore, Southern analysis clearly indicates that RETS are spread out all over the tobacco chromosomes. From these results, we have presented a working hypothesis that RETS offer a seed for telomere formation in the repair of chromosomal damages. In order to know functions of telomere, we constructed the first mobilizable YAC (yeast artificial chromosome) in which oriT (origin of transfer) is inserted between two telomeres. This plasmid was successfully transferred from Escherichia coli bacterium into Saccharomyces cerevisae yeast with the help of mob (mobilization) and tra (transfer) genes on a helper plasmid. However, the transconjugant yeasts had not linear YACs but circular YACs although OriT between telomeres was nicked during trans-kingdom conjugation. This indicates that 5'end of linearlized YAC might be blocked or anchored by something like mob protein moiety which locates in a conjugation tube.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] K. Suzuki, Y. Yamagiwa. T. Matsui, K. Yoshida: "Restriction enzymes-resistant high molecular weigtht telomeric DNA fragments in tabacco" DNA Research. 1. 129-138 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A. Mahmood, T. Kimura, M. Takenaka, K. Yoshida: "The construction of mobilizable YACs and their direct conjugative transfer from E. coli to yeasts" Nucleic Acids Symp.34. 45-46 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A. Mahmood, T. Kimura, M. Takenaka, K. Yoshida: "The construction of novel mobilizable YAC plasmids and their behavior during trans-kingdom conjugation between bacteria and yeasts" Genetic Analysis. 13(accepted.). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Suzuki, Y.Yamagiwa, T.Matsui, K.Yoshida: "Restriction enzymes-resistant high molecular weight telomeric DNA fragments in tabacco" DNA Research. 1. 129-138 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Mahmood, T.Kimura, M.Takenaka, K.Yoshida: "The construction of mobilizable YACs and their direct conjugative transfer from E.coli to yeasts" Nucleic Acids Symp.34. 45-46 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Mahmood, T.Kimura, M.Takenaka, K.Yoshida: "The construction of novel mobilizable YAC plasmids and their behavior during trans-kingdom conjugation between bacteria and yeasts" Genetic Analysis. 13, (accepted). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Suzuki, Y. Yamagiwa, T. Matsui, K. Yoshida: "Restriction enzymes-resistant high molecular weight telomeric DNA fragments in tabacco" DNA Research. 1. 129-138 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] A. Mahmood, T. Kimura, M. Takenaka, K. Yoshida: "The construction of mobilizable YACs and their direct conjugative transfer from E. coli to yeasts" Nucleic Acids Symp.34. 45-46 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A. Mahmood, T. Kimura, M. Takenaka, K. Yoshida: "The construction of novel mobilizable YAC plasmids and their behavior during trans-kingdom conjugation between bacteria and yeasts" Genetic Analysis. 13(accepted.). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Suzuki,Y.Yamagiwa,T.Matsui,K.Yoshida: "Restriction enzymes-resistant high molecular weight telomeric DNA fragments in tobacco" DNA Research. 1. 129 :138 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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