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Cytokinin Signal Transduction

Research Project

Project/Area Number 06804051
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionNara Institute of Science and Technology (1995-1996)
Akita Prefectural College of Agriculture (1994)

Principal Investigator

SANO Hiroshi  Research and Education Center for Genetic Information, Prfessor, 遺伝子教育研究センター, 教授 (20178809)

Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Keywordsnutrient signals / protein kinase / cytokinin / phytohormone / プロテインキナーゼ / WPK4 / イネ / 栄養飢餓 / 組換えタバコ / 情報伝達 / タンパク質リン酸化 / 植物ホルモン
Research Abstract

(1) A gene encoding a protein kinase was isolaed from wheat seedings, and designated as wpk4.
(2) The WPK4 protein was expressed in E.coli and Bacullo virus system.
(3) The enzymatic activity was evaluated using expressed proteins, and shown to possess autophosphorylation activity and MBP kinase activity in the presence of manganese ions.
(4) A specific antibody was raised against expressed WPK4.
(5) The enzyme specifically phosphorylated 65 kDa and 17.6 kDa proteins extracted from wheat seedings.
(6) Under poor nutrient conditions, transcripts of wpk4 greatly accumulated in seedlings.
(7) Upon transferring seedlings grown under nutrient-poor media into nutrient rich-media, transcripts of wpk4 rapidly disappeared.
(8) Cytokinin treatment of seedlings induced accumulation of transcripts of wpk4.
(9) The results suggest that WPK4 is a member of the nutrient signal transduction system, and that cytokinin functions in it as an activator of the system.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] H. SANO: "Light and nutritional regulation of transcoipts encoding a wheat protein kinase is mediated by Oytolcilins" Proc. Natl. Acnd. Sui. NSA. 91. 2582-2586 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 佐野 浩 他: "カイトカイニンの情報伝達" 植物の化学調節. 31. 103-112 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sano, H., and Youssefian, S.: "Light and nutritional regulation of transcripts encoding a wheat protein kinase is mediated by cytokinis" Proc.Natl.Acad.Sci.USA. 91. 2582-2586 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sano, H., Hara, K., Y.Ikeda and N.Koizumi: "Cytokinin signal transduction." Chemical Regulation Of Plant Growth. 31. 103-112 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 佐野 浩: "サイトカイニンの情報伝達" 植物の化学調節. 31. 103-112 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 佐野 浩 他: "サイトカイニンの情報伝達(予定)" 植物の化学調節. 31. (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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