| Project/Area Number |
06804055
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物生理・代謝
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| Research Institution | Toyama University |
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Principal Investigator |
NOGUCHI Munenori TOYAMA UNIV., FAC.SCI., ASSISTANT PROFESSOR, 理学部, 助教授 (30019004)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | CILIARY MOVEMENT / PARAMECIUM / PROTOZOA / ARGININE KINASE |
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| Research Abstract |
To clarify the ATP supplying system in protozoan cilia, we investigated some phosphagen metabolizing enzymes in Paramecium. Adenylate kinase and arginine kinase activities were detected in both cell body and cilia. Intracellular concentration of phosphoarginine was estimated at about 0.4 mM from the assay of the phosphoarginine contents in Paramecium cells. Ciliary beating was induced by the estimated 0.4 mM of phosphoarginine in the presence of ADP in ciliated sheets of cell cortex from Paramecium. These results indicate that a phosphoarginine shuttle contributes to supplying ATP in live Paramecium cilia.
We tried to isolate arginine kinase isoenzymes from cell body and cilia of Paramecium caudatum. Aithough the arginine kinases from Paramecium were unstable and rapidly lost their activities, we partially purified arginine kinases from deciliation medium and cilia using anion exchange chromatography, hydroxylapatite chromatography and gel filtration. The molecular weights of arginine kinases from cell body and cilia were estimated 46kDa and 42kDa, respectively. These isoenzyme must contribute to supplying energy as members of phosphoarginine shuttle system.
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