| Project/Area Number |
06806001
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | Kobe University |
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Principal Investigator |
HOSAKA Kazuyoshi Kobe University, Experimental Farm, Associate Professor, 農学部・附属農場, 助教授 (60222428)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Potato / Linkage map / RFLP / RAPD / Self-incompatibility |
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| Research Abstract |
A self-compatible (SC) variant of a wild diploid potato species Solanum chacoense, which is normally self-incompatible (SI), was investigated for self-compatibility. It was crossed with a SI cultivated diploid potato species S.phureja. The F_1 progenies segregated to be SC vs.SI and diallele crosses were made among them. Self-compatibility was tested in a selfed family of a parental SC variant and in sib-mated and selfed families of F_1 progenies. All the data suggest that there is a single dominant gene (Sli gene) inhibiting S gene expression in the pollens. Plants having a Sli gene produced pollens which are compatible to both its own parent and the others. The Sli gene has been maintained as a heterozygous condition through 8 times of selfed generations implying that homozygotes might be associated with lethality.
One of the F_1 progenies was crossed as a male parent with one of selective clones from a S.stenotomum-S.phureja population, resulting in a segregating population consisted with 116 hybrid plants. Self-compatibility was assessed by selfing each of the hybrids. Sixty-six of them were SC,while 35 were SI,showing a significant distortion from an expected Mendelian ratio of 1 : 1. A genetic linkage map was constructed using DNA markers to localize the Sli gene. A total of 38 RAPD and 35 RFLP markers identified 52 loci on 11 linkage groups, among which the Sli gene was mapped at a distal end of chromosome 12. As A locus has been localized on chromosome 1 on the potato RFLP map, it is confirmed that the Sli gene is independent to S locus.
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