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EFFECT OF ADP-RIBOSYLATION OF p33 AND ACTIN ON POLYMORPHONUCLEAR LEUKOCYTE DIFFERENTIATION

Research Project

Project/Area Number 06807011
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionSHIMANE MEDICAL UNIVERSITY

Principal Investigator

SHIMOYAMA Makoto  SHIMANE MEDICAL UNIV.VICE PRESIDENT, 医学部, 副学長 (30084859)

Co-Investigator(Kenkyū-buntansha) YAMADA Kazuo  SHIMANE MEDICAL UNIV.BIOCHEMISTRY.INSTRUCTOR, 医学部, 助手 (30240005)
TERASHIMA Masaharu  SHIMANE MEDICAL UNIV.BIOCHEMISTRY.INSTRUCTOR, 医学部, 助手 (40227517)
TSUCHIYA Mikako  SHIMANE MEDICAL UNIV.BIOCHEMISTRY.PROFESSOR, 医学部, 教授 (90188582)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsPOST-TRANSLATIONAL MODIFICATION / ADP-RIBOSYLTRANSFERASE / ADP-RIBOSYLATED PROTEIN / ARGININE
Research Abstract

By the support of the Grant-in-Aid for Scientific Research 0680711, we obtained the following results. 1) Arginine-specific ADP-ribosyltransferase from chicken heterophils ADP-ribosylated Arg-28 and Arg-206 of G-actin, and Arg-28 of F-actin in vitro, without affecting the ATPase activity, indicating the modification of Arg-206 prevents the polymerization of actin. 2) Arginine-specific ADP-ribosyltransferase from chicken heterophils ADP-ribosylated Arg-113, Arg-250 and Arg-254 of p33 in vitro. Though 4 mol of ADP-ribose can be stoichiometrically incorporated into one mol of p33, besides these three arginine residues, five candidates (Arg-8, Arg-37, Arg-235, Arg-269, and Arg-285) were detected. Partial reduction of intramolecular disulfide bonds of p33 by sulfhydryl agent, necessary for the ADP-ribosylation, may play a role to change the conformation of the p33 and accessibility of the transferase. 3) Amino terminal sequence of p33-half-2 was SPPFPQQ,indicating that the cleavage site is upstream of Ser-149 in the spacer region of p33.4) cDNA cloning revealed that chicken bone marrow cells have at least two ADP-ribosyltransferases. 5) Enzyme activity or expression of the transferase and amount of the protein or message of p33 found in peripheral polymorphonuclear leukocytes were lower than one tenth of those detected in bone marrow cells.
Though we established the method to detect p33 and p33-halves using anti-p33 antibodies, we have not yet demonstrated the activity of endopeptidase which specifically cleaves p33 and generates p33-halves, in vitro or in reconstituting system. Further studies are required to identify the endopeptidase and elucidate its importance.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Mikako Tsuchiya: "Cloning and expression of cDNA for arginine-specific ADP-ribosyltransferase from chicken bone marrow cells" J. Biol. Chem.269. 27451-27457 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikako Tsuchiya: "target protein for eucaryotic arginine-apecific ADP-ribosyltransferase" Mol. Cell. Biochem.138. 113-118 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tomoya Ohno: "Vortex-mixing-induced inactivation of arginine-specific ADP-ribosyltransferase activity and re-activation of the less-active form by dethiothreitol plus NaCl under anaerobic conditions" Biochem. Mol. Biol.32. 213-220 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuo Yamada: "Automodification of arginine-specific ADP-ribosyltransferase purified from chicken peripheral heterophils and alteration of the transferase" Arch. Biochem. Biophys.308. 31-36 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 土屋美加子: "NOとADP-リボシル化" THE LUNG perspectives. 3. 66-70 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikako Tsuchiya: "Assay of arginine-specific adenosine-5'-diphosphate-ribosyltransferase by capillary electrophoresis" Anal. Biochem.224. 486-489 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaharu Terashima: "ADP-ribosylation of Arg28 and Arg206 on the actin molecule by chicken arginine-apecific ADP-ribosyltransferase" Eur. J. Biochem.231. 242-249 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jose Luis Rosa: "Role of the N-terminal region in covalent modification of 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase: comparison of phosphorylation and ADP-ribosylation" Biochem. J.309. 119-125 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikako Tsuchiya: "A newly identified GPI-anchored arginine-specific ADP-ribosyltransferase activity in chicken spleen" Biochem. Biophys. Res. Commun.214. 760-764 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Chikako Yamamori: "ADP-ribosylation of myelin basic protein and inhibition of phospholipid vesicle aggregation" Enzyme Protein. 48. 202-212 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tomoya Ohno: "Detection of arginine-ADP-ribosylated protein using recombinant ADP-ribosylarginine hydrolase" Anal. Biochem.231. 115-122 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuyoshi Miyaoka: "Activation of Clostridium botulinum C3 exoenzyme-catalyzed ADP-ribosylation of RhoA by K^+ in a Mg^<2+> -dependent manner" J. Biochem.119. 200-207 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 土屋美加子: "脊椎動物のアルギニン特異的ADP-リボシル転移酵素" 実験医学. 14. 304-307 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikako Tsuchiya: "Cloning and expression of cDNA for arginine-specific ADP-ribosyltransferase from chicken bone marrow cells" J.Biol.Chem.269-44. 27451-27457 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikako Tsuchiya: "Target protein for eucaryotic arginine-specific ADP-ribosyltransferase" Mol.Cell.Biochem.138. 113-118 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tomoya Ohno: "Vortex-mixing-induced inactivation of arginine-specific ADP-ribosyltransferase activity and reactivation of the less-active form by dithiothreitol plus NaCl under anaerobic conditions" Biochem.Mol.Biol.Int.32-2. 213-220 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuo Yamada: "Automodification of arginine-specific ADP-ribosyltransferase purified from chicken peripheral heterophils and alteration of the transferase activity" Arch.Biochem.Biophys.308-1. 31-36 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikako Tsuchiya: "NO and ADP-ribosylation" THE LUNG perspectives. 3-2. 66-70 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikako Tsuchiya: "Assay of arginine-specific adenosine-5^1-diphosphate-ribosyltransferase by capillary electrophoresis" Anal.Biochem.224-3. 486-489 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaharu Terashima: "ADP-ribosylation of Arg28 and Arg206 on the actin molecule by chicken arginine-specific ADP-ribosyltransferase" Eur.J.Biochem.231-1. 242-249 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] J.L.Rosa: "Role of the N-terminal region in covalent modification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase : comparison of phosphorylation and ADP-ribosylation" Biochem.J.309-1. 119-125 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikako Tsuchiya: "A newly identified GPI-anchored arginine-specific ADP-ribosyltransferase activity in chicken spleen" Biochem.Biophys.Res.Commun.214-3. 760-764 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cikako Yamamori: "ADP-ribosylation of myelin basic protein and inhibition of phospholipid vesicle aggregation" Enzyme Protein. 48. 202-212 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tomoya Ohno: "Detection of arginine-ADP-ribosylated protein using recombinant ADP-ribosylarginine hydrolase" Anal.Biochem.231-1. 115-122 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuyoshi Miyaoka: "Activation of Clostridium botulinum C3 exoenzyme-catalyzed ADP-ribosylation of RhoA by K^+ in a Mg^<2+>-dependent manner" J.Biochem.119-1. 200-207 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikako Tsuchiya: "Vertebrate arginine-specific ADP-ribosyltransferase" Experimental Medicine. 14-2. 304-307

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikako Tsuchiya: "Assay of arginine-specific adenosine-5^1-diphosphate-ribosyltransferase by capillary electrophoresis" Anal.Biochem.224. 486-489 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masaharu Terashima: "ADP-ribosylation of Arg28 and Arg206 on the actin molecule by chicken arginine-specific ADP-ribosyltransferase." Eur.J.Biochem.231. 242-249 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Jose Luis Rosa: "Role of the N-terminal region in covalent modification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase : comparison of phosphorylation and ADP-ribosylation" Biochem.J.309. 119-125 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mikako Tsuchiya: "A newly identified GPI-anchored arginine-specific ADP-rebosyltransferase activity in chicken spleen" Biochem.Biophys.Res.Commun.214. 760-764 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Chikako Yamamori: "ADP-ribosylation of myelin basic protein and inhibition of phospholipid vesicle aggregation" Enzyme Protein. 48. 202-212 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tomoya Ohno: "Detection of arginine-ADP-ribosylated protein using recombinant ADP-ribosylarginine hydrolase" Anal.Biochem.231. 115-122 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsuyoshi Miyaoka: "Activation of Clostridium botulinum C3 exoenzyme-catalyzed ADP-ribosylation of RhoA by K^+ in a Mg^<2+>-dependent manner" J.Biochem.119. 200-207 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 土屋美加子: "脊椎動物のアルギニン特異的ADP-リボシル転移酵素" 実験医学. 14. 304-307 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazuo Yamada: "Automodification of arginine-specific ADP-ribosyltransferase purified from chicken peripheral heterophils and alteration of the transferase activity" Arch.Biochem.Biophys.308. 31-36 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Mikako Tsuchiya: "Cloning and expression of cDNA for arginine-specific ADP-ribosyltransferase from chicken bone marrow cells" J.Biol.Chem.269. 27451-27457 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Mikako Tsuchiya: "Assay of arginine-specific ADP-ribosyltransferase by capillary electrophoresis" Anal.Biochem.244. 486-489 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Jose L.Rosa: "Role of the NH2-terminal region in covalent modification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase" Biochem.J.(印刷中). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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