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Studies of the effect of antifungal agcnts on a filamentous fungus by a biocell tracer system

Research Project

Project/Area Number 06807026
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Bacteriology (including Mycology)
Research InstitutionChiba University

Principal Investigator

TAGUCHI Hideaki  Ciba Univ. Res. Cent. Patho. Fungi. and Microb Toxicoses, Div. of Phylogenesis of Pathogenic Fungi Research Associate, 真核微生物研究センター, 助手 (70092070)

Co-Investigator(Kenkyū-buntansha) NIHIMURA Kazuko  Ciba Univ. Res. Cent. Patho. Fungi. and Microb Toxicoses, Div. of Phylogenesis o, 真核微生物研究センター, 教授 (00114314)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
KeywordsBiocell Tracer / Aspergillus fumigatus / growth rate / antifungal agent / synergistic effect / single hypha / BioCell-Tracer / Single hypha / miconazole
Research Abstract

The biocell tracer system developped by Dr. Matsuoka is an apparatus to measure a hyphal growth. Using this apparatus, the effect of antifungal agents such as amphotericin B (AMPH), flucytosin (5-FC), miconazole (MCZ) and fluconazole (FLCZ) on hypae was studied. The bottom of culture vessel was coated with poly-L-lysine and 10^3 conidia of Aspergillus fumigatus were inoculated at the center of the vessel. Then liquid medium was poured into the vessel and the growth rate of a hypha adhering to the bottom was measured. When the growth rate dropped dto 0 after the addition of an antifungal agent, we judged the hyphal growth was completely inhibited. In particular this apparatus is very useful to study the synergistic effect of antifungal agents. The synergistic effect of AMPH and 5-FC was singnificantly influenced by timing of their addition to the culture vessel. When 250 mug/ml of 5-FC was used first into the medium and 2 mug/ml of AMPH was used 10 min later, the hyphal growth dropped immediately. However, when AMPH was added first and 5-FC was added 10 min later, the hyphal growth dropped gradually. When AMPH (2 mug/ml) and 5-FC (250 mug/ml) were used independently, no morphological changes of the fungal hyphae were observed. On the other hand, their combination at these concentrations showed a distinctinhibitory activity. This method is useful for antifungal activity testing of filamentous fungi.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] 田口英昭: "BioCell TracerによるAspergillus fumigatusに対するmiconazoleの抗真菌効果の検討" 日本医真菌学会雑誌. 36. 33-37 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Taguchi: "Studies on the synergistic effect of amphotericin B and 5-fluorocytosine on the growth rate of single hyphace of Aspergillus fumigatus by a biocell tracer system" Mycoscience. 36. 341-344 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Taguchi, M.Miyaji, M.L.Xu, K.Nishimura and Y.Yoshida: "Studies on an antifungal effect of miconazole on Aspergillus fumigatus by biocell tracer" Jpn. J.Med. Mycol.36. 33-37 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Taguchi, M.Miyaji, K.Nishimura and M.L.Xu: "Studies on the synergistic effect of amphotericin B and 5-fluorocytosine on the growth rate of single hyphae of Aspergillus fumigatus by a biocell tracer system" Mycoscience. 36. 341-344 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 田口英昭: "BioCell TracerによるAspergillus fumigatusに対するmiconazoleの抗真菌効果の検討" 日本医真菌学会雑誌. 36. 33-37 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Taguchi: "Studies on the synergistic effect of amphotericin B and J-flu cytosine on the growth rate of single hyhae of Aspergillus fumigatus by biocell" Mycoscience. 36. 341-344 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 田口英昭 等: "BioCell-TracerによるAspergillus fumigatusに対するmiconazoleの抗真菌効果の検討" 日本医真菌学雑誌. 36. 33-37 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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