Development of the rapid detection method of methicillin resistant Staphylococcus aureus (MRSA)
| Project/Area Number |
06807040
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Public health/Health science
|
|---|
| Research Institution | Osaka Prefectural Institute of Public Health |
|---|
Principal Investigator |
SAKAGAMI Yoshikazu Osaka Prefectural Institute of Public Health, Pharmaceutical Affairs, Senior Researcher, 薬事指導部, 主任研究員 (50192084)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAJIMURA Keiji 大阪府立公衆衛生研究所, 薬事指導部, 主任研究員 (40250336)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
|
|---|
| Keywords | MRSA / NEW DNA CHEMIPROBE / mec A / PCR / メチシリン耐性黄色ブドウ球菌(MRSA) / 抗生物質 / MIC値の低下 / DNAプローブ / MIC値の分布範囲 |
|---|
| Research Abstract |
[Object] The development of a rapid detection method of MRSA was performed for the regulation of MRSA infection, one of the severe problem in hospital of Japan.
[Methods] MIC values against methicillin were measured with 130 strains of MRSA isolated from 7 hospitals in Japan. Repeated cultivation was performed for 10 times (for 10 days) for obsevation of MRSA-resistant pattern. A rapid detection method of MRSA was developed by using NEW DNA CHEMIPROBE method with using MRSA and methicillin-sensitive(resistant-falling strains ; MIC value of methicillin was below 12.5 mu g/ml) strains, and compared with the detection method of mec A gene by PCR.
[Results] The detction method by NEW DNA CHEMIPROBE on using MRSAs and methicillin-sensitive(resistant-falling starins) strains was almost similar to the detection method of of mec A gene by PCR.
[Conclusion] From the test results, it would suggest that the detection method by NEW DNA CHEMIPROBE had good detection sensitivity and almost equal to the detection of mec A gene by PCR.Furthermore, it would contribute to MRSA detection field.
|
Report
(3 results)
Research Products
(1 results)
