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Induction of immunological tolerance in the mice reconstituted by human T cells

Research Project

Project/Area Number 06807046
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionNagasaki University

Principal Investigator

KAWABE Yojiro  Nagasaki University, School of Medicine, Assistant Professor, 医学部, 助手 (50244059)

Co-Investigator(Kenkyū-buntansha) EGUCHI Katsumi  Nagasaki University, School of Medicine, Associate Professor, 医学部, 助教授 (30128160)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
KeywordsSCID mice / human T cells / Immunological tolerance / cell adhesion / superantigen / RT-PCR / Vbeta TCR / RT-PCR / T細胞 / 細胞移行 / ヒトーマウスキメラ
Research Abstract

Early disappearance of Vbeta 8.1,2+T cells from murine peripheral blood lymphocytes was observwd after Staphylococcal enterotoxine B (SEB) inoculation. Disappearance of such a Vbeta 8.1,2+T cells of peripheral blood lymphocytes was observed within one hour and reach maximum at 2 hours after SEB injection. The decrease of Vbeta 8.1,2+T cells was not caused by apoptosis of the cells, or the downregulation of cell surface expression of T cell receptor, but caused by specific T cell adhesion to endothelial cells. These obervation was confirmed by in vitro culture system. Adherence of murine splenic T cells to human endothelial cells was augmented by SEB,specifically in Vbeta 8.1,2+T cells.
Human peripheral T cells were inoculated into SCID mice intraperitoneally after irradiation and anti-asialo GM1 antibody was inoculated 3 times/week. Human peripheral T cells were found in murine spleen 3 weeks after human peripheral T cell inoculation. The human T cells recovered from murine spleen were weakly responsive against SEB.SEB inoculation against human peripheral T cell reconstituted mice did not cause specific human T cell deletion. Immunological tolerance against SEB after SEB inoculation of human T cell reconstituted mice was not achieved, probably because reconstituted human T cells were already activated by murine antigens, and hardly to be activated in vitro stimulation with SEB.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] S.Sakito et al.: "Serum cytokine patients with rheumatioid arthritis-correlation of interferon-γ and tumor necrosis factor α with the characteristics of peripheral blood mononuclear cells." Rheumatol Int. 15. 31-37 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Origuchi et al.: "Elevated levels of serum IgM anti-SEB antibody in patients with rheumatoid arthritis" Ann Rheum Dis. 54. 713-720 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Migita et al.: "FK506 augments activation induced programmed cell death of T lymphocytes in vivo." J Clin Invest. 210. 727-732 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Tsukada et al.: "Transforming growth factor β1 induces apoptosis cell death in cultured human umbilical vein endothelial cells with down-regulated expression of bcl-2." Biochem Biophys Res Commun. 210. 1076-1082 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Migita et al.: "The role of protein kinase in human synovial fibroblast growth." Biochem Biophys Res Commun. 210. 1066-1075 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Migita et al.: "Defective TCR-mediated signaling in anergic T cells." J Immunol. 155. 5083-5087 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Migita, K.Eguchi, Y.Kawabe, A.Mizokami, T.Tsukada, S.Nagataki: "Prevention of anti-CD3 monoclonal antibody induced thymic apoptosis by protein tyrosine kinase inhibitors." J Immunol. 153. 3457-3465 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Origuchi, K.Eguchi, Y.Kawabe, A.Mizokami, H.Ida, S.Nagataki: "Synovial cells are potent antigen-presenting cells for superantigen, staphylococcal enterotoxin B (SEB)." Clin Exp Immunol. 99. 345-351 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Sakito, Y.Ueki, K.Eguchi, Y.Kawabe, S.Nagataki: "Serum cytokines in patients with rheumatoid arthritis-correlation of interferon gamma and tumor necrosis factor alpha with the characteristics of peripheral blood mononuclear cells-" Rheumatol Int. 15. 31-37 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Origuchi, K.Eguchi, Y.Kawabe, I.Yamashita, A.Mizokami, H.Ida, S.Nagataki: "Elevated levels of serum IgM anti-SEB antibody in patients with rheumatoid arthritis." Ann Rheum Dis. 54. 713-720 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Migita, K.Egushi, Y.Kawabe, S.Nagataki: "Tyrosin phosphorylation participates in peripheral T cell activation and programd cell death in vivo." Immunology. 85. 550-555 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Migita, K.Eguchi, Y.Kawabe, T.Tsukada, A.Mizokami, S.Nagataki: "FK506 augments activation-induced programd cell death of T lymphocytes in vivo." J Clin Invest. 96. 727-732 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Tsukada, K.Eguchi, K.Migita, Y.Kawabe, A.Kawakami, N.Matsuoka, H.Takashima, A.Mizokami, S.Nagataki: "Transforming growth factor beta1 induces apoptotic cell death in cultured human umbilical vein endothelial cells with down-regulated expression of bcl-2." Biochem Biophys Res Commun. 210. 1076-1082 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Migita, K.Eguchi, T.Tsukada, Y.Kawabe, T.Aoyagi, S.Nagataki: "The role of protein kinase in human synovial fibroblast growth." Biochem Biophys Res Commun. 210. 1066-1075 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Migita, K.Eguchi, Y.Kawabe, T.Tsukada, I.Ichinoge, S.Nagataki: "Defective TCR-mediated signaling in anergic T cells." J Immunol. 155. 5083-5087 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Yamasaki, K.Eguchi, Y.Kawabe, T.Tsukada, S.Nagataki: "Wegener's Granulomatosis Overlapped with Takayasu Arteritis." Clin Rheum. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Tsuboi, K.Eguchi, A.Kawakami, N.Matsuoka, Y,Kawabe, T.Aoyagi, K.Maeda, S.Nagataki: "Fas antigen expression on synovial cells was down-regulated by interleukin-1beta." Biochem Biophys Res Commun. 218. 280-285 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Migita, K.Eguchi, T.Tsukada, Y.Ichinose, Y.Kawabe, T.Aoyagi, S.Nagataki: "The effect of the immunosuppressant rapamycin on the growth of rheumatoid arthritis synovial cells." Clin Exp Immunol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masako Kita, Katsumi Eguchi, Yojiro Kawabe, Toshiaki Tsukada, Kiyoshi Migita, Atsushi Kawakami, Naoki Matsuoka, Shigenobu Nagataki: "Staphylococcal enterotoxin B specific adhesion of murine splenic T cells to human endothelial cell line" Immunology. (accepted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Sakito et al.: "Serum cytokines in patients with rheumatoid arthritis-correlation of interferon γ and tumor necrosis factor α with the characteristics of peripheral blood mononuclear cells-" Rheumatol Int. 15. 31-37 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Origuchi et al.: "Elevated levels of serum IgM anti-SEB antibody in patients with rheumatoid arthritis." Ann Rheum Dis. 54. 713-720 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Migita et al.: "FK506 augments activation-induced programmed cell death of T lymphocytes in vivo." J Clin Invest. 96. 727-732 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Tsukada et al.: "Transforming growth factor β1 induces apoptotic cell death in cultured human umbilical vein endothelial cells with down-regulated expression of bcl-2." Biochem Biophys Res Commun. 210. 1076-1082 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Migita et al.: "The role of protein kinase in human synovial fibroblast growth." Biochem Biophys Res Commun. 210. 1066-1075 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Migita et al.: "Defective TCR-mediated signaling in anergic T cells." J Immunol. 155. 5083-5087 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Origuchi: "Synovial cells are potent antigen-presenting cels for superantigen,staphylecocoal enterotoxin B(SEB)" Clin Exp.Immunol. in press.

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Migita: "Prevention of anti-CD3 monoclonal antibody-induced thymic apoptosis by protein tyrosine kinase inhibitors" J.Immunol. 153. 3457-3465 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Shimada: "Inteleukin 4 increases human synovial cell expression of VCAM-1 and T cell binding" Ann Rheum Dis. 53. 601-607 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 河部庸次郎: "スーパー抗原投与による実験的脳脊髄炎の増悪" 臨床免疫. 26. 1385-1390 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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