The contribution of nitroxide on neonatal polymorphonuclear cells (PMNs)
| Project/Area Number |
06807066
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | KANSAI MEDICAL UNIVERSITY |
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Principal Investigator |
TANIUCHI Shoichiro Kansai Medical University, Medicine Assistant Professor, 医学部, 講師 (70171832)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAYA Junji Kansai Medical University, Medicine Instructor, 医学部, 助手 (80247923)
KODERA Urara Kansai Medical University, Medicine Instructor, 医学部, 助手 (90198614)
KINOSHITA Yo Kansai Medical University, Medicine Associate Professor, 医学部, 助教授 (10105778)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Neonates / Polymorphonuclear leukocytes / Nitric oxide / Superoxide |
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| Research Abstract |
To elucidate the susceptibility to bacterial infections in neonates, we evaluated the effects of nitric oxide (NO) on MCLA and LCL in PMNs with 12 cord bloods and 8 adults using L-arginene, a NO precursor and L-NMMA,a NOS inhibitor. There was no significant difference of the LCL intensity on MCLA n two groups in PMA stimulated PMNs. Adding L-arginine to PMNs, no significant reduction of the LCL intensity was observed in both groups. In the presence of L-NMNA,the LCL intensity significantly increased in the both groups (P<0.05).
The LCL intensity of lumino-dependent chemiluminescence (LDC) in cord PMNs stimulated with PMA significantly decreased than that of LDC in adult PMNs (P<0.001). Adding L-arginene, the LCL intensity of LCL significant increased in both groups (adult, neonate ; p<0.05). Similar increases in both groups were found (adult ; 11%, neonate ; 9%). In the presence of L-NMNA,the LCL intensity significantly increased in the both groups (P<0.01). Remarkable increases of the LCL were observed in adult PMNs, compared with cord PMNs.
From our results, a reduction of the LCL intensity of LDC in cord PMNs may be partly related to a decrease of NO production, suggesting that a cause of the susceptibility to bacterial infection could be ascribed to a decreased NO production in neonates.
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Report
(3 results)
Research Products
(4 results)
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[Publications] Fujiwara, T., Taniuchi, S., Hattori, K., Kobayashi, T., Kinoshita, Y.and Kobayashi, Y.: "Effect of immunoglobulin therapy on phagocytosis by polymorphornuclear leucocytes in wholeblood of neonates." Clin Exp Immunol. 107. (1997)
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