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Molecular biological study on the significance of keratinocyte-substratum interaction for their existence and proliferation-comparison with cancer cells-

Research Project

Project/Area Number 06807071
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionJichi Medical School

Principal Investigator

KATAYAMA Hiroshi  Jichi Medical School, Dermatology, Lecturer, 医学部, 講師 (50142401)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsCell-Substratum interaction / Anchorage-dependency / Detachment / Keratinocyte / Squamous cell carcinoma cell / Focal adhesion kinase / Tyrosine phosphorylation / Laminin / 細胞・基質間接着 / 浮遊状態 / Cell-substratum interaction / focal contact / 接着分子 / bFGF
Research Abstract

Normal cells in culture other than blood cells can not grow without attachment to the substratum. This is a phenomenon called anchorage-dependency. Several malignant cells are known to be anchorage-independent. Focal adhesion kinase (FAK) is a recently described non-receptor type protein-tyrosine kinase that localizes at cell-substratum attachment sites named focal adhesion. FAK is supposed to be activated, possibly by tyrosine autophosphorylation, when cells attach to extracellular matrix and transmit signals for growth and differentiation. In this study we examined FAK expression and its tyrosine phosphorylation in both attached and detached forms of cultured normal human keratinocytes and their malignant counterpart, human epidermal carcinoma cell line (DJM-1), in order to clarify how cell-substratum interaction and FAK are involved in their survival and growth. DJM-1 kept detached and dissociated by elastase survived at least 3 weeks. Survived cells (approximately 30% of the total) … More rapidly proliferated when released from detachment. Their FAK assayd at one week in detachment was tyrosine-phosphorylated in a degree slightly decreased than that when they were attached to wells. Majority of keratinocytes kept detached by elastase disappeared within a week. They lost the ability to proliferate when kept detached only for 3 days. FAK in keratinocytes detached by addition of elastase for 24h was hardly tyrosine-phosphorylated. These facts suggested a close association of sustained FAK tyrosine phosphorylation with their prolonged survival in detached DJM-1. Detached DJM-1 expressed laminin on their surfaces as well as integrins alpha_2beta_1, alpha_3beta_1 and alpha_6beta_4, all of which mediate keratinocyte adhesion to laminin. Thus their interactions on cell membrane might have playd a role in FAK tyrosine phosphorylation in detached DJM-1. Curiously, detached DJM-1 had fungus-like appearance. Results obtained in this study could greatly contribute to human efforts to eradicate cancers. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Hiroshi Katayama etal.: "Prolonged survival and re-proliferative ability are closely associated with sustained tyrosinephosphorylation of focal adhesion kinase in detached epidermal carcinoma cell line,DJM-1." Cancer Research. 近日中に投稿予定.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 片山 洋: "皮膚とプロスタグランジン" 現代医療. 27. 173-179 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 出光俊郎.片山洋ら: "メルケル細胞癌" 皮膚科の臨床. 38. 123-127 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小菅綾子.片山洋ら: "Ichthyosis bullosa of Siemens の1例" 臨床皮膚科. 49. 1083-1086 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 片山 洋: "扁平苔癬" 皮膚疾患最新の治療'97-'98'(南江堂). 印刷中. (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 片山 洋: "ベーチェット病" 今日の治療指針(1997年版)(医学書院). 印刷中. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroshi Katayama, Yasuhiro Yamane, Yusuke Furukawa, Seiichi Kitagawa, Yuji Yamaguchi, Hideo Yaoita: "Prolonged survival and re-proliferative ability are closely associated with sustained tyrosine phosphorylation of focal adhesion kinase in detached epidermal carcinoma cell line, DJM-1" Cancer Research. (to be submitted soon).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroshi Katayama et al: "Prolonged survival and r-eproliferative ability are closely associated with sustained tyrosine phosphorylation of facal adhesion kinase in detached epidermal carcinoma cell line, DJM-1" Biochem. Biophys, Res, Commun.(近日中に投稿予定).

    • Related Report
      1995 Annual Research Report
  • [Publications] 片山洋: "皮膚とプロスタグランジン" 現代医療. 27. 173-179 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 出光敏郎、片山洋ら: "メルケル細胞癌" 皮膚科の臨床. 38. 123-127 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小菅綾子、片山洋ら: "Ichthyosis bullosa of Siemensの1例" 臨床皮膚科. 49. 1083-1086 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 片山洋: "扁平苔癬" 皮膚疾患最新の治療'97-'98(南江堂). (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 片山洋: "ベ-ツェット病" 今日の治療指針(1997年版)(医学書院). (印刷中). (1997)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiroshi KATAYAMA et al: "Detachment of cultured normal human Keratinocytos by contact with TNFα-stimulated neutrophils in the presence of platelet activiting factor" Journal of Investigative Dermatology. 103. 187-190 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 片山洋・他: "Platelet activating factorのcell-cell contactを介するcell-cell interactionへの関与" 脂質生化学研究. 36. 31-34 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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