| Project/Area Number |
06807115
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ISOBE Yasushi Tokyo Medical and Dental University, Medicine, assistant professor, 医学部, 助手 (90176264)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Cancer / Therapy / Membrane integrity / Cell fusion / Xenoplantability / 異種移植性 / 異種可殖性 / 赤血球膜 / ポリエチレングリコール |
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| Research Abstract |
Tumor cell (PNET cell line, registrated as NBsusSR at Riken Cell Bank) and human RBC cells were fusioned with PEG (M.W.1500) in order to investigate whether destruction of membrane integrity of tumor cell leads to reduction of malignant properties of tumor cell. Morphological investigations showed fusioned cells enlarge slightly and were somewhat susceptible to a few kinds of enviromental flactuations. These cells dose not show any further growth and disappeared after three weeks. These fusioned cells did not show any transplantability to Balb C nu/nu mice and SCID mice.
Afterall, it is confirmed that cell fusion procedure may reduce malignant properties of cancer cell and may leads cancer patients to cure. There are many problems in the clinical apprication of usage of cell fusion technique as cancer therapy stratege. This investigation indicates that we should pay some attention to membrane integrity when we design new chemotherapy protocol or seek new anticancer agents.
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