| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
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| Research Abstract |
A sensitive and simple method of high-performance liquid chromatography with pulsed amperometric detection (HPLC-PAD) has been developed to determine red cell sugars and polyols, a potentially useful indicator of diabetic complications. Red cells were hemolyzed with deionized water by freezing and thawing method, and deproteinized with ultrafilter Centricon-10. Red cell sorbitol and myo-inositol were separated with Sugar-Pak Pb columns, eluted with deionized water at 80ﾟC,and detected using PAD.
The red blood cell (RBC) sorbitol levels were 17.27<plus-minus>9.12 nmol/gHb (Mean <plus-minus> SD) in normal subjects (n=11), 31.00<plus-minus>12.29 nmol/gHb in diabetic subjects without microangiopathy (n=16), 61.10<plus-minus>25.11 nmol/gHb in diabetic subjects with microangiopathy (n=6). The RBC myo-inositol revels were 91.58<plus-minus>27.45 nmol/gHb in normal subjects (n=24), 151.71<plus-minus>53.1 nmol/gHb in diabetic subjects without microangiopathy (n=41), 253.64<plus-minus>79.10 nmol/gHb in diabetic subjects with microangiopathy (n=14). Concentrations of RBC myo-inositol in diabetic subjects without microangiopathy were significantly higher than those in normal individuals (p<0.01). Moreover, a significant increase in myo-inositol levels was observed in diabetic patients who had microangiopathy, as compared to those without microangiopathy, in all examined samples (p<0.01) including glucose or HbAlc-matched samples (p=0.0018). On the basis of these results, we suggest that determination of RBC norbitol and myo-inositol by the HPLC-PAD method is useful for evaluating diabetic mi
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