| Project/Area Number |
06808070
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | TOKYO MEDICAL AND DENTAL UNIVERSITY |
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Principal Investigator |
TATSUMI Hitoshi TOKYO MEDICAL AND DENTAL UNIVERSITY,MED.RES.INST., ASISTANT PROF., 難治疾患研究所, 助手 (20171720)
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| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Yoshifumi TOKYO MEDICAL AND DENTAL UNIVERSITY,MED.RES.INST., PROF., 難治疾患研究所, 教授 (20014144)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
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| Keywords | Nano Meter / Neuron / Growth cone / synaps / Laser trap / membrane / protein / Nano-meter / molecule / 微分干渉顕微鏡 / ビデオ画像処理 / ナノメートル計測 / 光ピンセット / レーザー光 / 光学技術 / 神経生理学 / 金コロイド粒子 |
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| Research Abstract |
Neurons protrude growth cones during development and also during axon sprouting after damage. Evoked release of acetylcholine (ACh) from the growth cones of central cholinergic neurons in culture was monitored using whole-cell patch clamp recording from ACh-receptor-rich neuron placed close to the growth cones as a sensor of ACh release. Simultaneous video-enhanced DIC imaging of growth cones showed contacts between growth cones and the sensor neuron after ACh release. Adhesion was also observed between growth cones and latex beads, when growth cones were exposed to high potassium solution. After beads attached on the growth cone membrane, 1 pN of laser optical pressure was required for moving the beads about 0z1 to 1 mum on the growth cone surface. The changes in the surface viscosity of the growth cone membrane was estimated by analyzing the video images of laser trapped beads on it.
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