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Nuclear transplantation experiment in Xenopus oocytes using B cell nuclei : Is nuclei with changed genom totipotent?

Research Project

Project/Area Number 06808078
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionJapan Women's University

Principal Investigator

NAGATA Saburo  Japan Women's University, Faculty of Science, Associate Professor, 理学部, 助教授 (00164434)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsNuclear transplantation / Cell differentiation / Genomic equivalence / Xenopus / B細胞 / 細胞文化 / ゲノムの不変性
Research Abstract

Nuclear transplantation experiments in Xenopus oocytes provide an unequivocal evidence for genomic equivalence in cell differentiation. One of the exceptions for genomic equivalence in vertebrates is recombination of immunoglobulin (Ig) and T cell receptor genes. In this project, I asked whether nuclei from antibody-producing B cells, which has a recombined Ig gene may support the development upon injected into an enucleated oocyte.
Splenocytes producing anti-DNP antibodies were isolated by adsorption on a DNP-coated plate, and the nuclei were transferred into UV-enucleated oocytes. The nuclear host eggs initiated cleavage and some of them developed beyond gastrulation.
However, no embryos reached hatching stages. Various tissues from the embryos developed with transplanted cytogenetically-labeled nucleus were transplanted into embryos, and allowed to develop to swimming larvae. Analysis for the nuclear marker demonstrated differentiation of various types of host-derived cells, i. e., cells with the B cell nucleus. The results indicate that nuclei with a recombined Ig gene can support differentiation of various types of cells.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Nagata,S.,Chiba,N.,Takeuchi,K.,Watanabe,K.: "An immunoglobulin superfamily cell adhesion molecule,XF3,in Xenopus : primary structure and developmental expression." Zoological Science. 11. 86- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagata,S.,Chiba,N.,Takeuchi,K.,Watanabe,K.: "Primary structure and expression of XF3,a Xenopus homologue of the immunoglobulin superfamily cell adhesion molecule contaction (F3/F11)." Gene. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagata, S., Chiba, N., Takeuchi, K., Watanabe, K.: "An immunoglobulin superfamily cell adhesion molecule, XF3, in Xenopus : primary structure and developmental expression." Zool. Sci.11(6). 84 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagata, S., Chiba, N., Takeuchi, K., Watanabe, K.: "Primary structure and expression of XF3, a Xenopus homologue of the immunoglobulin superfamily cell adhesion molecule contactin (F3/F11)." Gene. in press (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagata, S. Chiba, N., Takeuchi, K., Watanabe, K.: "An immunoglobulin superfamily cell adhesion molecule, XF3,in Xenopus: primary structure and developmental expression." Zoological Sciene. 11. 84 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nagata, S., Chiba, N., Takeuchi, K., Watanabe, K.: "Primary structure and expression of XF3, a Xenopus homologue of the immunoglobulin superfamily cell adhesion molecule contactin(F3/F11)" Gene.(発表予定).

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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