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Surveys on new human malaria in South East Asia

Research Project

Project/Area Number 07041159
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionField Research
Research InstitutionNagoya University School of medicine

Principal Investigator

KAWAMOTO Fumihiko  Dept.of Med.Zool., School of Medecine Nagoya Univ.Lecturer, 医学部, 講師 (40115556)

Co-Investigator(Kenkyū-buntansha) TANTULAR Indah  Dept.of Parasitol., Fac.of Med., Airlagga Univ., Indonesia, 医学部, 講師
WIRJATMADI Bamban  Dept.of Parasitol., Fac.of Med., Airlangga Univ., Indonesia, 医学部, 講師
DACHLAN Yoes.P  Dept.of Parasitol., Eac.of Med., Airlangga Univ., Indonesia, 医学部, 助教授
WONGSRICHANA チャンスタ  アメリカ陸軍医科学研究所, 研究員
YAMAMOTO Toshimichi  Dept.of Forns.Sc., Sch.Med.Nagoya Univ., 医学部, 助手 (50260592)
近藤 高明  名古屋大学, 医学部, 助手 (00195900)
松岡 裕之  自治医科大, 講師 (10173816)
KIMURA Masatsugu  Lab.of biophysics, Med.Sch.Osaka City Univ., 医学部, 助手 (60195378)
綿矢 有佑  岡山大学, 薬学部, 教授 (90127598)
WONGSRICHANALAI Chansuda  Dept.of Immunol., Armed Forc.Res.Inst.Med.Sc., Bangkok
CHANSUDA Won  アメリカ陸軍医科学研究所, 研究員
WIRJATMADI バンバン  アイルランガ大学, 医学部, 講師
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1996: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1995: ¥3,600,000 (Direct Cost: ¥3,600,000)
KeywordsMalaria / Molecular diagnosis / Acridine orange staining / New variant / Thailand / Indonesia / Myanmar / China / 分子疫学 / 熱帯熱マラリア / 四日熱マラリア / リボゾームRNA遺伝子
Research Abstract

Field survey of malaria was conducted in Thailand, Indonesia, Myanmar and China using an acridine orange (AO) staining method. Diagnostic results were compared with those by microtiter plate hybridization (MPH), and a nested PCR methods.
1. In Thailand, a total of 548 samples were obtained, and many P.malariae and P.ovale infections diagnosed by the AO method were confirmed by the MPH and/or nested PCR methods. Their prevalence reached about 20% and 2.2%, respectively. In addition, three samples of P.ovale infections were AO-positive but MPH-negative. Analysis of the target sequences revealed that these isolates were the same mutated type (two nucleotide deletion and one nucleotide substitution) with that found in Vietnam. On the other hand, P.falciparum isolates were found to possess a point mutation at the target region in the 18S rRNA gene.
2. In China, many P.malariae infections were also detected from the Sichuan Province by the three methods. By the nested PCR method, a long deletion from amplified PCR product was found at the probe region, indicating that this may be a new mutated type of P.malariae. Later, the same mutated form was detected from Thailand, Indonesia, and Myanmar, indicating that it is widely distributing in SEA.
3. In Myanmar, many P.malariae and P.ovale infections were also detected. The mutated types of both of P.malariae and P.ovale which had been found in Vietnam was also detected. In contrast, in Indonesia only six samples of P.malariae and only two of P.ovale were found. One sample of P.malariae was the same new type, but others were normal.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] F.Kawamoto et al.: "Sequence variation in the 18S rRNA gene, a target for PCR-based malaria diagnosis, in Plasmodium ovale from southern Vietnam." J.Clinical Microbiology. 34. 2287-2289 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C.Wongsrichanalai et al.: "In vitro susceptibility of Plasmodium falciparum isolates in Vietnam to artemicinin derivatives and other antimalarials." Acta Tropica. In press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kimura et al. (in preparation): "Identification of the four species of human malaria parasites by nested PCR that targets variant sequences in the small subunit rRNA gene." Parasitology International.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.U.Ferreira et al.: "Stable patterns of allelic diversity at the merozoite surface protein-1 of Plasmodium falciparum in clinical isolates from the southern Vietnam" J.Eukaryotic Microbiology. (in preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Z.Mian et al.: "High Prevalence of Plasmodium malariae and P.ovale in malaria patients along the Thai-Myanmar Border" J.Clinical Microbiology. (in preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] F.Kawamoto et al.: "Comparison of the morphologies of same malaria parasites stained with acridine orange and then Giemsa" J.Clinical Microbiology. (in preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] F.Kawamoto: "Sequence variation in the 18S rRNA gene,a target for PCR-based malaria diagnosis,in Plasmodium ovale from southern Vietnam." J. Clinical Microbiolosy. 34,9. 2287-2289 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] C.Wongsrichanalai: "In vitro susceptibility of Plasmodium falciparum isolates in Vietnam to artemicinin derivatives and other antimalarials." Acta Tropica. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] M. Kimura: "Identification of the four species of human malaria parasites by nested PCR that targets variant sequences in the small subunit rRNA gene." Parasitolology International. (発表予定).

    • Related Report
      1996 Annual Research Report
  • [Publications] M.U.Ferreira: "Stable patterns of alleic diversity at the merozoite surface protein-1 of Plasmodium falciparum in clinical isolates from the southern Vietnam" J.Eukaryotic Microbiology. (発表予定).

    • Related Report
      1996 Annual Research Report
  • [Publications] Z.Mian: "High Prevalence of Plasmodium malariae and P.ovale in malaria patients along the Thai-Myanmar Border" J.Clinical Microbiology. (発表予定).

    • Related Report
      1996 Annual Research Report
  • [Publications] F.Kawamoto: "Comparison of the morphologies of,same malaria parasites stained with acridine orange and then Giemsa" J. Clinical Microbiology. (発表予定).

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Kimura,M.Zhou,Q.Liu,O.Kaneko,S.Isomura,F.Kawamoto: "Identification of four human malaria parasites by a nested PCR method" Molecular and Biochemical Parasitology Year of Publication:. (submitted).

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Zhou,Q.Liu,M.Kimura,C.Wongsrichanalai,W.Suwonkerd,K.Panart,S.Prajakwong,et al.: "High revalence of Plasmodium malariae and detection of Plasmodium ovale at the border between Thailand and Myanmer" American Journal Tropical Medicine and Hygine Year of Publication:. (submitted).

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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