| Co-Investigator(Kenkyū-buntansha) |
BARZAGA Nina g. University of the Philippines Mannila, College of Public Health Associate Profes, 公衆衛生学部, 助教授
PICHARTPIYAKUL Chiang Mai University, Faculty of Medicine Lecurer, 医学部, 講師
SOETJIPTO Airlangga University, School of Medicine Lecturer, 医学部, 講師
KATAYAMA Yuko Kobe University, School of Medicine Assistant, 医学部, 助手 (10224461)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Research Abstract |
1. Subtype analysis of hepatitis C virus (HCV) in Thailand.
(1)Northern part of Thailand (Chiang Mai) :
Anti-HCV-positive sera obtained from patients with chronic hepatitis, liver cirrhosis and hepatoma were subjected to reverse transcription-polyumerase chain reaction (RT-PCR) to analyze NS5B region sequences of the HCV genome, based on which subtype classification of the HCV isolates were pertormed. Among patients with chronic hepatitis, HCV-3a and HCV-1a (each 20%) were most common, followed by HCV-1b (16%). An isolate could not be classified into a known subtype. Among patients with liver cirrhosis, HCV-3a (44%) was most common, followed by HCV-1a, HCV-1b (each 19%) and HCV-3b (6%). Among patients with hepatoma, HCV-1a (40%) and HCV-3a (20%) were common ; however, precise comparison with the other patients groups could not be made due to the insufficient number of samples from hepatoma patients.
(2)Southern part of Thailand (Hat Yai) :
Anti-HCV-positive sera obtained from blood donors and patients with chronic liver disease were analyzed as described above. About 25 sera have been analyzed and the remaining 50 sera remain to be analyzed.
2.Subtype analysis of HCV in Indonesia.
Among blood donors in Surabaya, HCV-2a (52%) was most common, followed by HCV-1b (15%), HCV-1a (7%), HCV-1d (7%) and HCV-3g (4%). Among patients on maintenance hemodialysis, HCV-1a and HCV-1b (each 31%) were most common, with HCV-2a (9%) and HCV-1b (3%) being less common. Among patients with hepatoma, HCV-1b (57%) was most common, followed by HCV-1d (19%) and HCV-2a (5%).
3. Subtype analysis of HCV in the Philippines.
Among blood donors in Metro Manila, HCV-1b (50%) was most common, followed by HCV-1a (31%) and HCV-2a (5%). Among inmates, on the other hand, HCV-1a (68%) was most common, followed by HCV-1b (11%), HCV-2a (5%) and HCV-2b (5%). HCV-1b was less prevalent in inmates than in blood donors. HCV-3a or HCV-1d was not isolated in the Philippines.
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