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STUDY ON ECOLOGY AND EVOLUTION OF ROTAVIRUS

Research Project

Project/Area Number 07041164
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionField Research
Research Field Virology
Research InstitutionSapporo Medical University

Principal Investigator

URASAWA Shozo  Sapporo Med.Univ., Dept.of Hygiene, Professor, 医学部, 教授 (00045379)

Co-Investigator(Kenkyū-buntansha) WU Huixia  Tongji Med.Univ., Dept.of Environ, Microbioly., Associate Professor, 助教授
PONGSUWANNA Yaowapa  NIH,Thailand, Enteric Virus Laboratory, Chief, 室長
KOBAUYASHI Nobumichi  Sapporo Med.Univ., Dept.of Hygiene, Assistant Professor, 医学部, 講師 (80186759)
URASAWA Tomoko  Sapporo Med.Univ., Dept.of Nursing, Professor, 保健医療学部, 教授 (90045378)
TANIGUCHI Koki  Sapporo Med.Univ., Dept.of Hygiene, Associate Professor, 医学部, 助教授 (40094213)
VAOWAPA Pnog  タイ国立衛生研究所(NIH), 室長
WU Huixia  中華人民共和国同済医科大学, 助教授
MUZAHED U.  バングラデシュ国立農科大学, 獣医学部, 教授
CELIA C. Ca  フィリピン国立ウイルス研究所, 上級特別研究員
YAOWAPA Pong  タイ国立衛生研究所(NIH), 室長
AHMED Muzahe  バングラデシュ国立農科大学, 獣医学部, 教授
CARLOS Celia  フィリピン国立ウイルス研究所, 上級特別研究員
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 1997: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1996: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
KeywordsHUMAN ROTAVIRUS / ANIMAL ROTAVIRUS / PEDIATRIC DIARRHEA / VIRAL ANTIGEN / VIRAL GENE / GENE SEQUENCE / EVOLUTION / C群ロタウイルス / 遺伝子塩基配列 / VP7遺伝子
Research Abstract

We collected fecal specimens from humans (mostly pediatric diarrhea cases) and animals in 4 Asian cuontries. With these specimens, screening of rotavirus (RV), antigenis characterization (by ELISA and PCR) of RVs detected, isolation of selected RV strains, and nucleotide sequence determination of genome segment of selected strains were carried out in order to elucidate the epidemiology of human rotavirus infections and the ecology and evolution of rotaviruses in human and mammals.
1.In Wuhan, China, 3050 diarrhea samples were collected between 1995 and 1997. Nine hundred thirty-six specimens (31%) positive for group ARV were subjected to further antigenic and genetic analysis mentioned above. While serotype G1 strains predominated throughout the study period, the serotype distribution of human RVs differed markedly from year to year. No G4 strain was detected. A few strains having antigenic properties unusual for human RV were examined for the nucleotide sequence of VP8 and NSP5 gene se … More gments. It was of note that the NSP5 sequence of two Chinese strain was more closely related to that of porcine than to human RVs.
2.In Muntinlupa, Philippines, 906 samples were collected from patients with diarrhea between 1995 and 1997. One hundred eighty-one samples (19.9%) were RV-positive. During the study period dominant serotype of RV changed rapidly from G1 to G2, then to G4 and lastly to G3. However, G12 strains, which had been reported to be prevalent only in Philippines at the end of 1987, were not detected in the present study. Besides these, one strain esch of group, C RV and picobirna virua was found.
3.In 560 fecal specimens collected from piglets in Thailand, 23 group A RVs, 1 group B RV,2 group C RVs and 2 picobirna birusres were detected. Fourteen (61%) of 23 group A RVs were serotype G10 which hitherto has not been found in porcine RVs.Comparative sequence analysis of VP4 and VP7 genes together with RNA-RNA hybridization showed that these G10 porcine strain were very closely related to G10 bovine RVs isoland previously in the same country, indicating strongly transmission of RV between cows and pigs. The serotype distribution of human RVs were also examined with 171 human RV-positive specimens collected in Bangkok.
4.Screening of animal rotavirus in Bangladesh with 590 porcine and 270 bovine fecal specimens as well as 50 specimens from other animals gave negative result except for the detection of 2 porcine and one bovine RVs. Human specimens were also collected from 840 diarrhea patients in Mymensingh, Bangladesh. One hundred twelve RV-positive specimens were examined for their serotypes. Detected serotypes were G1, G2 and G4 in order of frequency. Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (51 results)

All Other

All Publications (51 results)

  • [Publications] Koki Taniguchi: "Nondefective rotavirus mutants with an NSP1 gene which has a deletion of 500 nucleotides,including a cysteine-rich zinc finger motif-encoding region(nucleotides 156 to 248),or which has a nonsense codon at nucleotides 153 to 155." J.Virol.70. 4125-4130 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazunobu Kojima: "Species-specific and interspecies relatedness of NSP1 sequences in human,porcine,feline,and equine rotavirus strains." Arch.Virol.141. 1-12 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nobumichi Kobayashi: "Selection of rotavirus VP7 gene in the genetic background of simian rotavirus SA11:implications for rotavirus reassortant vaccine development." Antiviral Res.31. 185-190 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nobumichi Kobayashi: "G(VP7)serotype-dependent preferential VP7 gene selection detected in the genetic background of simian rotavirus SA11" Arch.Virol.141. 1167-1176 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yaowapa Pongsuwanna: "Serological and genomic characterization of porcine rotaviruses in Thailand:detection of a G10 porcine rotavirus." J.Clin.Microbiol.34. 1050-1057 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazunobu Kojima: "Sequence analysis of normal and rearranged NSP5 genes from human rotavirus strains isolated in nature:implications for the occurrence of the rearrangement at the step of plus strand synthesis." Virology. 224. 446-452 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Koki Taniguchi: "Structure and function of rotavirus NSP1." Arch.Virol. 12[Supple]. 53-58 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Ishizaki: "The distribution of G and P types within isolates of bovine rotavirus in Japan." Vet.Microbiol.48. 367-372 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shuji Nakata: "Outbreaks of nosocomial rotavirus gastro-enteritis in a paediatric ward." Eur.J.Pediatr.155. 954-958 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Sakawaki: "Occurrence of a single type strain(G3,P[12])of equine rotavirus in foals on a racehorse farm from 1986 to 1993." Res.Bull.Obihiro Univ.20. 103-111 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mitsutaka Kuzuya: "Survey of human Group C royaviruses in Japan during the winter of 1992 to 1993." J.Clin.Microbiol.36. 6-10 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Huixia Wu: "Serological and genomic characterization of human rotaviruses detected in China" J.Med.Virol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jun-ichi Okada: "Preferential selection of heterologous G3-VP7 gene in the genetic background of simian rotavirus SA11 detected by using a homotypic single-VP7 gene substitution reassortant." Antiviral Res.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 谷口 孝喜: "ウイルス学 「レオウイルス」" 朝倉書店(畑中正一 編)(in press),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 浦沢 正三: "微生物学教科書「ロタウイルス・レオウイルス」" 文光堂(畑中正三・嶋田甚五郎 編)(in press),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Taniguchi, K.Kojima, S.Urasawa: "Nondefective rotavirus mutants with an NSP1 gene which has a deletion of 500 nucleotides, including a cysteine-rich zinc finger motif-encoding region (nucleotides 156 to 248), or which has a nonsense codon at nucleotides 153 to 155." J.Virol. 70. 4125-4130 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Kojima, K.Taniguchi, N.Kabayashi: "Species-specific and interspecies relatedness of NSP1 sequences in human, porcine, feline, and equine rotavirus strains." Arch.Virol.141. 1-12 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Kabayashi, J.Okada, K.Taniguchi, T.Urasawa, S.Urasawa: "Selection of rotavirus VP7 gene in the genetic background of simian rotavirus SA11 : implications for rotavirus reassortant vaccine development." Antiviral Res.31. 185-190 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Kabayashi, K.Taniguchi, K.Kojima, T.Urasawa, S.Urasawa: "G (VP7) serotype-dependent preferential VP7 gene selection detected in the genetic background of simian rotavirus SA11" Arch.Virol.141. 1167-1176 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Pongsuwanna, K.Taniguchi, M.Chiwakul, T.Urasawa, F.Wakasugi, C.Jayavasu, S.Urasawa: "Serological and genomic characterization of porcine rotaviruses in Thailand : detection of a G10 porcine rotavirus." J.Clin.Microbiol.34. 1050-1057 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Kojima, K.Taniguchi, T.Urasawa, S.Urasawa: "Sequence analysis of normal and rearranged NSP5 genes from human rotavirus strains isolated in nature : implication for the occurrence of the rearrangement at the step of plus strand synthesis." Virology. 224. 446-452 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Taniguchi, K.Kojima, N.Kobayashi, T.Urasawa, S.Urasawa: "Structure and function of rotavirus NSP1." Arch.Virol.12 [Supple]. 53-58 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Ishizaki, T.Sakai, T.shirahata, K.Taniguchi, T.Urasawa, S.Urasawa, H.Goto: "The distribution of G and P types within isolates of bovine rotavirus in Japan." Vet.Microbiol.48. 367-372 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Nakata, N.Adachi, S.Ukae, K.Kogawa, K.Numata, S.Urasawa, S.Chiba: "Outbresks of nosocomial rotavirus gastro-enteritis in a paediatric ward." Eur.J.Pediatr.155. 954-958 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Sakawaki, H.Ishizaki, M.Takagi, G.Masuda, K.Taniguchi, T.Urasawa, S.Urasawa, H.Goto, T.Shirahata: "Occurrence of a single type strain (G3, P[12]) of equine rotavirus in foals on a racehorse farm from 1986 to 1993." Res.Bull.Obihiro Univ.20. 103-111 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Kazuya, R.Fijii, M.Hamano, M.Yamada, K.Shinozaki, A.Sasagawa, S.Hasegawa, H.Kawamoto, K.Matsumoto, A.Kawamoto, A.Itagaki, S.Funatsumaru, S.Urasawa: "Survey of human Group Crotaviruses in Japan during the winter of 1992 to 1993." J.Clin.Microbiol.36. 6-10 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Wu, K.Taniguchi, T.Urasawa, S.Urasawa: "Serological and genomic characterization of human rotaviruses detected in China" J.Med.Virol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.Okada, N.Kabayashi, K.Taniguchi, S.Urasawa: "Preferential selection of heterologous G3-VP7 gene in the genetic backgroud of simian rotavirus SA11 detected by using a homotypic single-VP7 gene substitution reassortant." Antiviral Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Wu Huixia: "Serological and genomic characterization of human rotaviruses detectede in China" J.Med.Virol.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Okada Junichi: "Preferential selection of heterologous G3-VP7 gene in the genetic background of simian rotavirus SA11 detected by using a homotypic single-VP7 genesubstitution reassortant" Antiviral Res.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Mitsutaka Kuzuya: "Survey of Human Group C Rotaviruses in Japan during the Winter of 1992 to 1993" J.Clin Microbiol. 36. 6-10 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sakawaki Hiromi: "Occurrence of a single type strain (G3,P[12]) of equine rotavirus in foals on a recehorse farm from 1986 to 1993" Res.Bull.Obihiro Univ.20. 103-111 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 谷口 孝喜: "医科ウイルス学(大里外誉郎 編)南江堂" レオウイルス科(in press), (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Koki Taniguchi: "Nondefective rotavirus mutants with an NSP1 gene which has a deletion of 500 nucleotides, including a cysteine-rich zinc finger motif-encoding region (nucleotides 156 to 248), or which has a nonsense codon at nucleotides 153 to 155." J. Virol.70. 4125-4130 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kazunobu Kojima: "Species-specific and interspecies relatedness of NSP1 sequences in human, porcine, bovine, feline, and equine rotavirus strains." Arch. Virol.141. 1-2 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hiroshi Ishizaki: "The distribution of G and P types within isolates of bovine rotavirus in Japan." Vet. Microbiol.48. 367-372 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yaowapa Pongsuwanna: "Serological and genomic characterization of porcine rotaviruses in Thailand : Detection of a G10 porcine rotavirus" J. Clin. Microbiol.34. 1050-1057 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akihisa Fukushima: "Effect of MDP-Lys (L18) as a mucosal immunoadjuvant on protection of mucosal infections by Sendai virus and rotavirus." Vaccine. 14. 485-491 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kazunobu Kojima: "Sequence analysis of normal and rearranged NSP5 genes from human rotavirus strains isolated in nature : implications for the occurrence of the rearrangement at the step of plus strand synthesis." Virology. 224. 446-452 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shuji Nakata: "Outbreaks of nosocomial rotavirus gastroenteritis in a paediatric ward." Eur. J. Pediatr.155. 954-958 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 谷口孝喜: "KEY WORD 1997-'98 感染症 : 分子疫学" 先端医学社, 160-161 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Koki Taniguchi: "Diversity in rotavirus genomes." Sem.Virol.6. 123-131 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小林 宜道: "ウイルス検査法、各論-ロタウイルス" 臨床とウイルス. 23. 179-183 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 谷口 孝喜: "ウイルスの抗原構造" 病態生理. 14. 731-738 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 谷口孝喜: "タイにおけるロタウイルス感染の分子疫学研究-タイ国立衛生研究所研究促進プロジェクトに参加して-" 国際協力研究. 11. 65-76 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yaowapa Pongsuwanna: "Serological and genetical characterization of human and animal rotaviruses prevailing in Thailand" Sapporo Med.J.64. 1-21 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazunobu Kojima: "Species-specific and interspecies relatedness of NSP1 sequences in human,porcine,bovine,feline,and equine rotavirus strains." Arch.Virol.141(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Koki Taniguchi: "Non-defective rotavirus mutants with NSP1 gene which has a deletion of 500 nucleotides including a cysteine-rich Zn finger motif encoding region (nucleotide no.156-248) or which has a nonsense codon at nucleotide no.153-155." J.Virol.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yaowapa Pongsuwanna: "Serological and genomic characterization of porcine rotaviruses in Thailand : Detection of a G10 porcine rotavirus" Gen.Virol.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 浦沢 正三: "ウイルス実験プロトコール(ロタウイルス)" ジカルビュー社, 261-272 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 谷口 孝喜: "臨床DNA診断法(II.DNA 診断法による臨床応用、II-3.感染症 c.RNA ウイルス、2.ヒトロタウイルス)" 金原出版, 970-972 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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