• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of Molecular Mechanism of Blastic Crisis in Chronic Myelocytic Leukemia

Research Project

Project/Area Number 07042002
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionSpecial Cancer Research
Research InstitutionUniversity of Tokyo

Principal Investigator

HIRAI Hisamaru  Faculty of Med., University of Tokyo・lecturer, 医学部(病), 講師 (90181130)

Co-Investigator(Kenkyū-buntansha) HIRANO Naoto  Faculty of Med., University of Tokyo・research associate, 医学部(病), 医員
WITTE Owen  UCLA, 医学部, 教授
UENO Hiroo  Faculty of Med., University of Tokyo・research associate, 医学部(病), 医員
KUROKAWA Mineo  Faculty of Med., University of Tokyo・research associate, 医学部(病), 医員
OGAWA Seishi  Faculty of Med., University of Tokyo・research associate, 医学部(病), 医員
TANAKA Tomoyuki  Faculty of Med., University of Tokyo・assistant professor, 医学部(病), 助手 (50227154)
HANAZONO Yutaka  Faculty of Med., University of Tokyo・assistant professor, 医学部(病), 助手 (70251246)
MITANI Kinuko  Faculty of Med., University of Tokyo・assistant professor, 医学部(病), 助手 (50251244)
QWEN Witte  UCLA・professor
Project Period (FY) 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1995: ¥5,700,000 (Direct Cost: ¥5,700,000)
KeywordsChronic Myelocytic Leukemia / Blastic Crisis / Evi-1 Gene / Cell Cycle / p16INK4A Gene / p53 Gene / RB Protein / Tumor Suppressor Gene
Research Abstract

Evi-1 is a transforming gene originally identified in a common integration site of murine leukemia retrovirus and mapped in human chromosome 3q26. It is overexpressed in retrovirus induced murine myeloid leukemias as well as human myeloid leukemias with 3q26 abnormalities, and thus thought to be responsible for both human and murine leukemogenesis. In this study, a possible involvement of the Evi-1 gene in blastic transformation of chronic myelocytic leukemia (CML) was examined by northern blot analysis, and the frequencies of its expression were compared between Japanese patients and American ones. Expression of the Evi-1 gene was detected in 48% of Japanese patients and 40% of American ones. Our results suggest that increased expression of the Evi-1 gene may play an important role in development of human leukemias, especially in progression from chronic phase to blastic crisis of CML even without 3q26 abnormalities.
It is now evident that the cell cycle machinery has a variety of elem … More ents negatively regulating cell cycle progression. Among these negative regulators in cell cycle control, however, only 4 have been shown to be consistently involved in development of human cancers as tumor suppressors : Rb (Retinoblastoma susceptibility protein), p53, and recently identified two cyclin-dependent kinase inhibitors, p16INK4A/MTS1 and p15INK4B/MTS2. Because there are functional interrelations among these negative regulators in the cell cycle machinery, it is particularly interesting to investigate the multiplicity of inactivations of these tumor suppressors in blastic transformation of CML.In order to address this point, we examined inactivations of these four genes in patients with blastic crisis of CML by Southern blot and PCR-SSCP analyzes. We also analyzed Rb protein expression by western blot analysis. The p16INK4A was homozygously deleted in 9% and 15% in Japanese patients and American ones. The p53 gene was mutated in 27% and 20%, and the RB protein was inactivated in 18% and 14% in Japanese patients and American ones, respectively. These results suggest that there are no significant differences in inactivation frequences of these tumor suppressors in blastic transformation of CML. Less

Report

(1 results)
  • 1995 Final Research Report Summary
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Tanaka T: "An acute myeloid leukemia gene,AML1,regulates hemopoietic myeloid cell differentiation and transcriptional activation antagonistically by two alternative spliced forms." EMBO J.14. 341-350 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka T: "Dual functions of the AML1/Evi-1 chimeric protein in the mechanism of leukemogenesis in t (3 ; 21) leukemias." Mol.Cell.Biol.15. 2383-2392 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitani K: "Cloning of several spieces of MLL/MEN chimeric cDNAs in myeloid leukemias with t (11 ; 19) (q23 ; p13.1) translocation." Blood. 85. 2017-2024 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitani K: "Growth inhibition of leukemic cells carrying the t (3 ; 21) by the AML 1/EVI-1 specific antisense oligonucleotide." Brit.J.Hematol.90. 711-714 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ogawa S: "Loss of the cyclin-dependent kinase 4-inhibitor (CDK4I ; p16 ; MTS1) gene is frequent in,and highly specific to lymphoid tumors in human hematopoietic malignancies." Blood. 86. 1548-1556 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kurokawa M: "The AML1/Evi-1 fusion protein generated in the t (3 ;21) translocation exhibits transforming activity on Ratl fibroblasts with dependence on the Evi-1 sequence." Oncogene. 11. 833-840 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka T: "An acute myeloid leukemia gene, AML1, regulates hemopoietic myeloid cell differentiation and transcriptional activation antagonistically by two alternative spliced forms." EMBO J.14. 341-350 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka T: "Dual functions of the AML1/Evi-1 chimeric protein in the mechanism of leukemogenesis in t(3 ; 21) leukemias." Mol. Cell. Biol.15. 2383-2392 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitani K: "Cloning of several spieces of MLL/MEN chimeric cDNAs in myeloid leukemias with t(11 ; 19) (q23 ; p13.1) translocation." Blood. 85. 2017-2024 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitani K: "Growth inhibition of leukemic cells carrying the t(3 ; 21) by the AML1/EVI-1 specific antisense oligonucleotide." Brit. J.Hematol.90. 711-714 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ogawa S: "Loss of the cyclin-dependent kinase 4-inhibitor (CDK4I ; p16 ; MTS1) gene is frequent in, and highly specific to lymphoid tumors in human hematopoietic malignancies." Blood. 86. 1548-1556 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kurokawa M: "The AML1/Evi-1 fusion protein generated in the t(3 ; 21) translocation exhibits transforming activity on Rat1 fibroblasts with dependence on the Evi-1 sequence." Oncogene. 11. 833-840 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hirai H: Elsevier Science Publishers B.V.Genetic alterations of oncogenes and anti-oncogenes in myelodysplastic syndrome (MDS) and MDS-derived leukemias. in Myelodysplastic syndromes. (eds. Nomura T and Yoshida Y), 229-236 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitani K: Elsevier Science Publishers B.V.AML1/EVI-1 fusion genes by the t (3 ; 21) (q26 ; q22) causes leukemic change of stem cell disorder. in Myelodysplastic syndromes. (eds. Nomura T and Yoshida Y), 205-216 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hirai H: Genetic alterations of oncogenes and anti-oncogenes in myelodysplastic syndrome (MDS) and MDS-derived leukemias. in Myelodysplastic syndromes. (eds. Nomura T and Yoshida Y). Elsevier Science Publishers B.V., 413 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitani K: AML1/EVI-1 fusion genes by the t (3 ; 21) (q26 ; q22) causes leukemic change of stem cell disorder. in Myelodysplastic syndromes. (eds. Nomura T and Yoshida Y). Elsevier Science Publishers B.V., 413 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi