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Studies of Floral Antitumor Medicines in Yunnan Province

Research Project

Project/Area Number 07042010
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionSpecial Cancer Research
Research InstitutionSchool of Science and Engineering Teikyo University

Principal Investigator

OTAKE Noboru  Department of Biosciences, School of Science and Engineering Teikyo University, Professor, 理工学部, 教授 (30013303)

Co-Investigator(Kenkyū-buntansha) 孫 啓時  瀋陽薬科大学, 助教授
陳 英傑  瀋陽薬科大学, 教授
姚 新生  瀋陽薬科大学, 教授
NISHIMURA Toshio  Department of Biosciences, School of Science and Engineering Teikyo University, 理工学部, 教授 (10013327)
SAKUDA Shohei  Department of Applied Biological Chemistry, The University of Tokyo, 農学部, 助教授 (80192087)
SUZUKI Akinori  Department of Applied Biological Chemistry, The University of Tokyo, 農学部, 教授 (90011907)
YAO Shin-Sheng  Shenyang Pharmacentical University, China
SUN Qi Shi  Shenyang Pharmacentical University, China
CHEN Ying-Jie  Shenyang Pharmacentical University, China
Project Period (FY) 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1995: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsAntitumor / Cytotoxity / Toxol Derivatives / BRM Activity / Macrophage Phagocytosis / Inhibition of Cell Cycles / P388 / ADM / K562 / tsFT210 cell
Research Abstract

It is well known that Yunnan Province has a variety of florae which provide many kinds of chinese medicines used in cancer therapy.
More recently, the taxol group derivatives have been developed for clinical use, attention was directed again to the antitumor constituents from plant origin.
This project concerns to the systemic search of the antitumor active substances from the extracts of plant samples collected in the Yunnan and Suzen Provinces.
Preparation of Plant Extracts
Each 50 plant samples were collected in the last of June and September, 1995 and were dried in natural condition. The dried samples were extracted with 60% aqueous methanol and boiling water, respectively.
The extracts were evaporated in vacuo, successively were lyophiled in toamorphous powders.
Test of Antitumor Activity
The antitumor activity in vitro was evaluated by cytotoxic activity against mouse leukemia P388/ADM cells and human leukemia K562/ADM cells.
Inhibitory Activity against Cell Cycles
The inhibitory activity against cell cycles was measured by cytoflowmetry method using mouse tsFT210 cells.
Screening for BRM Activity
The BRM activity was measured by a phagocytosis of mouse peritoneal macrophages in vitro.
Purification and Isolation of Active Principles
Purification and isolation of active principles were achieved by the use of chromatographic techniques in combination with HPLC.

Report

(1 results)
  • 1995 Final Research Report Summary
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 大岳 望: "抗生物質学" 養賢堂, 158 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大岳 望: "生合成の化学" 大日本図書, 235 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Taniguchi, T.Kataoka, H.Suzuki, M.Uramoto, M.Ando, K.Arao J.Magae, T.Nishimura, N.Otake and K.Nagai: "Costunolide and Dehydrocostus-lactone as Inhibitors of Hilling Function of Cytotoxic T-lymphocytes." Biosci.Biotech.Biochem. 59. 2064-2067 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Sakai, H.Kawai, M.kamishohara, A.Suzuki, T.Uchida, T.Tsuruo and N.Otake: "Synthesis and Antitumor Activities of Glycine-exchanged Analogs of Spicamycin." J,Antibiotics. 48. 504-508 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Sakai, K.Shindo, A.Odagawa, A.Suzuki, H.Kawai, K.Kobayashi, Y.Hayakawa, H.Seto and N.Otake: "Absolute Configuration of Spicamycin, an Antitumor Antibiotic Produced by Streptomyces alanosinicus." J,Antibiotics. 48. 899-900 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Nagata, H.Hatanaka, D.Kohda, H.Kataoka, H.Nagasawa, A.Isogai, H.Ishizaki, A.Suzuki and F.Inagaki: "Three-dimentional solution structure of bombyxin-II an insulin-like peptide of the silkmoth Bombyx mori : Structure comparison with insulin and relaxin." J.Mol.Biol. 253. 749-758 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Fukuchi, K.Furihata, J.Nakayama, T.Goudo, S.Takayama, A.Isogai and A.Suzuki: "Rotihibins, novel plant growth regulators from Streptomyces graminofaciens" J,Antibiotics. 48. 1004-1010 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Shiba, K.Hinata, A.Suzuki and A.Isogai: "Breakdown of Self-incompatibility in Brassica by the Antisense RNA of the SLG Gene." Proceedings of the Japan Academy. 71. 81-83 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] J.Nakayama, K.Yoshida, H.Kobayashi, A.Isogai, D.B.Clewell and A.Suzuki: "Cloning and characterization of a region of Enterococcus faecalis plasmid _<p.>PDl encoding pheromone inhibitor (ipd), pheromone sensitivity (traC), and pheromone shutdown (traB) genes." J.Bacteriol. 177. 5567-5573 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary

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Published: 1995-04-01   Modified: 2016-04-21  

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