| Project/Area Number |
07042010
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Special Cancer Research |
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| Research Institution | School of Science and Engineering Teikyo University |
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Principal Investigator |
OTAKE Noboru Department of Biosciences, School of Science and Engineering Teikyo University, Professor, 理工学部, 教授 (30013303)
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| Co-Investigator(Kenkyū-buntansha) |
孫 啓時 瀋陽薬科大学, 助教授
陳 英傑 瀋陽薬科大学, 教授
姚 新生 瀋陽薬科大学, 教授
NISHIMURA Toshio Department of Biosciences, School of Science and Engineering Teikyo University, 理工学部, 教授 (10013327)
SAKUDA Shohei Department of Applied Biological Chemistry, The University of Tokyo, 農学部, 助教授 (80192087)
SUZUKI Akinori Department of Applied Biological Chemistry, The University of Tokyo, 農学部, 教授 (90011907)
YAO Shin-Sheng Shenyang Pharmacentical University, China
SUN Qi Shi Shenyang Pharmacentical University, China
CHEN Ying-Jie Shenyang Pharmacentical University, China
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| Project Period (FY) |
1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1995: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | Antitumor / Cytotoxity / Toxol Derivatives / BRM Activity / Macrophage Phagocytosis / Inhibition of Cell Cycles / P388 / ADM / K562 / tsFT210 cell |
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| Research Abstract |
It is well known that Yunnan Province has a variety of florae which provide many kinds of chinese medicines used in cancer therapy.
More recently, the taxol group derivatives have been developed for clinical use, attention was directed again to the antitumor constituents from plant origin.
This project concerns to the systemic search of the antitumor active substances from the extracts of plant samples collected in the Yunnan and Suzen Provinces.
Preparation of Plant Extracts
Each 50 plant samples were collected in the last of June and September, 1995 and were dried in natural condition. The dried samples were extracted with 60% aqueous methanol and boiling water, respectively.
The extracts were evaporated in vacuo, successively were lyophiled in toamorphous powders.
Test of Antitumor Activity
The antitumor activity in vitro was evaluated by cytotoxic activity against mouse leukemia P388/ADM cells and human leukemia K562/ADM cells.
Inhibitory Activity against Cell Cycles
The inhibitory activity against cell cycles was measured by cytoflowmetry method using mouse tsFT210 cells.
Screening for BRM Activity
The BRM activity was measured by a phagocytosis of mouse peritoneal macrophages in vitro.
Purification and Isolation of Active Principles
Purification and isolation of active principles were achieved by the use of chromatographic techniques in combination with HPLC.
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