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Nucleation and Growth Kinetics in Protein Crystallization

Research Project

Project/Area Number 07044057
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

KOMATSU Hiroshi  Institute for Materials Research, Tohoku University, Professor, 金属材料研究所, 教授 (00108565)

Co-Investigator(Kenkyū-buntansha) THOMAS Bill  Center for Microgravity and Materials Research, University of Alabama, マイクログラビティーセンター, 助手
MUSCHOL Mart  アラバマ大学, マイクログラビティーセンター, 助手
VEKILOV Peter  Center for Microgravity and Materials Research, University of Alabama, マイクログラビティーセンター, 助手
ROSENBERGER Franz  Center for Microgravity and Materials Research, University of Alabama, マイクログラビティーセンター, 教授
SAZAKI Gen  Institute for Materials Research, Tohoku University, 金属材料研究所, 助手 (60261509)
NAKADA Toshitaka  Institute for Materials Research, Tohoku University, 金属材料研究所, 助手 (20237308)
MIYASHITA Satoru  Institute for Materials Research, Tohoku University, 金属材料研究所, 助手 (00219776)
OBINATA Masuo  Institute of development Aging and Cancer, Tohoku Univ., 加齢医学研究所, 教授 (10099971)
MUSHOL Martin  Center for Microgravity and Materials Research, University of Alabama
FRANZ Rosenb  アラバマ大学マイクログラビティーセンター, 教授
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥9,200,000 (Direct Cost: ¥9,200,000)
Fiscal Year 1996: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1995: ¥4,900,000 (Direct Cost: ¥4,900,000)
Keywordsproteins / lysozyme / crystal growth / impurity effect / two beam interferometry / light scattering / solubility / effect of magnetic field / タンパク質 / 濃度分布
Research Abstract

Achievements of this year are as follows ;
1. The Japanese group (J-group) measured solubilities of tetragonal, orthorhombic and triclinic form of hen egg white lysozyme (HEW lysozyme) using two beam interferometry.
2. J-group developed an interferometic method to measure the refractive index inside crystals (HEW lysozyme).
3. J-group got highly purified HEW lysozyme samples using HPLC.With this sample, they observed crystal interfaces using atomic force microscopy. This result was discussed with the American group (A-group) comparing with A-group's result.
4. J-group tried to perform crystallization of HEW lysozyme in a high magnetic field. And they found that it could control the nucleation rate and orientation of crystals.
5. Appoferritin (A-group) and ferritin (J-group) were highly purified and then crystallized. They showed again that removement of impurities is indispensable for growth of crystals of high quality. J-group made a morphodrom of ferritin.
6. A-group performed light scatte … More ring experiments of HEW lysozyme solution and discuss the aggregation process. They estimated the second virial coefficient of the solution and discussed relation between ion intensity and intermolecular interaction.
7. A-group precisely measured topography of vicinal surface with use of the laser interferometry and obtained time evolution of normal and step growth rates and slope of the vicinal surfaces.
8. A-group found that there existed a region where HEW lysozyme solution separated into two liquid phases. They made a phase diagram for this segregation with use of the light scattering technique.
9. In order to estimate the solution layr thickness where convection is ignored, A-grup performed computer simulation of the concentration profiles around a growing crystal changing thickness of the solution layr. J-group measured concentration profiles around a growing lysozyme crystal changing the thickness of the cell. These results were compared each other.
10. J-group measured solubility of HEW lysozyme in situ in high pressure. Pressure dependence of the growth rate was also studied. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (69 results)

All Other

All Publications (69 results)

  • [Publications] K.Kurihara,S.Miyashita,G.Sazaki,T.Nakada,Y.Suzuki and H.Komatsu: "Interferometric study on crystal growth of tetragonal lysozyme crystal." Journal of Crystal Growth. 166. 904-908 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] G.Sazaki,K.Kurihara,T.Nakada,S.Miyashita and H.Komatsu: "A novel approach to the solubility measurement of protein crystals by two-beam interferometry." Journal of Crystal Growth. 169. 355-360 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 佐崎 元、栗原和男、宮下 哲、小松 啓、中田俊隆: "二光束干渉法によるリゾチーム結晶周囲の濃度場観察" 日本結晶成長学会誌. 23. 413-421 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] G.Sazaki,E.Yoshida,H.Komatsu,T.Nakada,S.Miyashita and K.Watanabe: "Effects of a magnetic field on the nucleation and growth of protein crystals" Journal of Crystal Growth. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] E.Kashimoto,G.Sazaki,K.Hasegawa,T.Nakada,S.Miyashita and H.Komatsu: "Study on the crystallization of concanavalin A" Journal of Crystal Growth. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] E.Yoshida,G.Sazaki,T.Nakada,S.Miyashita,H.Komatsu and N.Niimura: "Morphodrom of ferritin crystal" Journal of Crystal Growth. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Sakuma,S.Miyashita,G.Sazaki,T.Nakada and H.Komatsu: "Motion of macro-steps induced on the surface of a lysozyme crystal" Journal of Crystal Growth. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Nakada,G.Sazaki,S.Miyashita and H.Komatsu: "In situ observation of growing surface of lysozyme crystal" Journal of Crystal Growth. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Yanagiya,G.Sazaki,T.Nakada,S.Miyashita and H.Komatsu: "Effect of a magnetic field on the orientation of lysozyme crystal" Journal of Crystal Growth. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Yanagiya,G.Sazaki,T.Nakada,S.Miyashita and H.Komatsu: "Effect of a magnetic field on the nucleation of lysozyme crystal" Journal of Crystal Growth. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] B.R.Thomas,P.G.Vekilov and F.Rosenberger: "Heterogeneity determination and purification of commercial hen egg white lysozyme." Acta Cryst.D52. 776-784 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] P.Vekilov,L.A.Monaco,B.R.Thomas,V.Stojanoff and F.Rosenberger: "Repartitioning of NaCl and protein impurities in lysozyme crystallization." Acta Cryst.D52. 785-798 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] F.Rosenberger: "Protein crystallization" J.Crystal Growth. 166. 40-54 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Muschol and F.Rosenberger: "Lack of evidence for prenucleation aggregate formation in lysozyme crystal growth solutions." J.Crystal Growth. 167. 738-747 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] F.Rosenberger,P.G.Vekilov,M.Muschol and B.R.Thomas: "Nucleation and crystallization of globular proteins-what do we know and what is missing?" J.Crystal Growth. 168. 1-27 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] P.G.Vekilov,J.I.D.Alexander and F.Rosenberger: "Nonliner response of layer growth dynamics in the mixed kinetics-bulk transport regime." Phys.Rev.E.54. 6650-6660 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] P.G.Vekilov,H.Lin and F.Rosenberger: "Unsteady crystal growth due to step-bunch cascading." Phys.Rev.E.55. 3202-3214 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Muschol and F.Rosenberger: "Liquid-liquid phase separation in supersaturated lysozyme solutions : Coupling to precipitate formation and crystallization." Biophys.J.(accepted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] F.Rosenberger,P.G.Vekilov,H.Lin and J.I.D.Alexander: "A rationale for system-dependent advantages and disadvantates of solution crystal growth at low gravity." Microgravity Sci.Technol.(accepted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Komatsu,S.Miyashita,T.Nakada,G.Sazaki and A.A.Chernov: ""An atomic level analysis of crystal growth mechanism in complex systems by means of nano-optical microscopy and AFM" in Advances in crystal growth mechanisms" Elsevier,Amsterdam, (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Kurihara, S.Miyashita, G.Sazaki, T.Nakada, Y.Suzuki and H.Komatsu: "Interferometric study on crystal growth o tetragonal lysozyme crystal" Journal of Crystal Growth. 166. 904-908 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] G.Sazaki, K.Kurihara, T.Nakada, S.Miyashita and H.Komatsu: "A novel approach to the solubility measurement of protein crystals by two-beam interferometry." Journal of Crystal Growth. 169. 355-360 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] G.Sazaki, K.Kurihara, S.Miyashu, H.Komatsu and T.Nakada: "In situ Observation of the Concentration Distribution around a Lysozyme Crystal by Two-beam Interferometry" Journal. of the Japanese.Association.for Crystal Growth.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] G.Sazaki, E.Yoshida, H.Komatsu, T.Nakada, S.Miyashita and K.Watanabe: "Effects of a magnetic field on the nucleation and growth of protein crystals" Journal of Crystal Growth. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] E.Kashimoto, G.Sazaki, K.Hasegawa, T.Nakada, S.Miyashita and H.Komatsu: "Study on the crystallization of concanavalin A" Journal of Crystal Growth. (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] E.Yoshida, G.Sazaki, T.Nakada, S.Miyashita, H.Komatsu and N.Niimura: "Morphodrom of ferritin" Journal of Crystal Growth. (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Sakuma, S.Miyashita, G.Sazaki, T.Nakada and H.Komatsu: "Motion o macro-steps induced on the surface of a lysozyme crystal" Journal of Crystal Growth. (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Nakada, G.Sazaki, S.Miyashita and H.Komatsu: "In situ observation of growing surface of lysozyme crystal" Journal of Crystal Growth. (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Yanagiya, G.Sazaki, T.Nakada, S.Miyashita and H.Komatsu: "Effect of a magnetic field on the orientation of lysozyme crystal" Journal of Crystal Growth. (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Yanagiya, G.Sazaki, T.Nakada, S.Miyashita and H.Komatsu: "Effect of a magnetic field on the nucleation of lysozyme crystal" Journal of Crystal Growth. (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] B.R.Thomas, P.G.Vekilov and F.Rosenberger: "Heterogeneity determination and purification of commercial hen egg white lysozyme." Acta Cryst.D52. 776-784 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] P.Vekilov, L.A.Monaco, B.R.Thomas, V.Stajanoff and F.Rosenberger: "Repartitioning of NaCl and protein impurities in lysozyme crystallization." Acta Cryst.D52. 785-798 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] F.Rosenberger: "Protein crystallization" J.Crystal Growth. 166. 40-54 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Muschol and F.Rosenberger: "Lack of evidence for prenucleation aggregate formation in lysozyme crystal growth solutions." J.Crysal Growth. 167. 738-747 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] F.Rosenberger, P.G.Vekilov, M.Muschol and B.R.Thomas: "Nucleation and crystallization of globular proteins-what do we know and what is missing?" J.Crystal Growth. 168. 1-27 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] P.G.Vekilov, J.I.D.Alexander and F.Rosenberger: "Nonlinear response of layr growth dynamics in the mixed kinetics-bulk transport regime." Phys.Rev.E.54. 6650-6660 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] P.G.Vekilov, H.Lin and F.Rosenberger: "Unsteady crystal growht due to step-bunch cascadig." Phys.Rev.E.55. 3202-3214 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Muschol and F.Rosenberger: "Liquid-liquid phase separation in supersaturated lysozyme solutions : Coupling to precipitate formation and crystallization." Biophys.J.(accepted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] F.Rosenberger, P.G.Vekilov, H.Lin and J.I.D Alexander: "A rationale for system-dependent advantages and disadvantates of solution crystal growth at low gravity." Microgravity Sci.Technol.(accepted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Komatsu, S.Miyashita, T.Nakada, G.Sazaki and A.A.Chernov: Elsevier, Amsterdam. "An atomic level analysis of crystal growth mechanism in complex systems by means of nano-optical microscopy and AFM" in advances in crystal growth mechanisms, (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Kurihara,S.Miyashita,G.Sazaki,T.Nakada,Y.Suzuki and H.Komatsu: "Interferometric study on crystal growth of tetragonal lysozyme crystal." Journal of Crystal Growth. 166. 904-908 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] G.Sazaki,K.Kurihara,T.Nakada,S.Miyashita and H.Komatsu: "A novel approach to the solubility measurement of protein crystals by two-beam interferometry." Journal of Crystal Growth. 169. 355-360 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 佐崎元、栗原和男、宮下哲、小松啓、中田俊隆: "二光束干渉法によるリゾチーム結晶周囲の濃度場観察" 日本結晶成長学会誌. 23. 413-421 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] G.Sazaki,H.Yoshida,H.Komatsu,T.Nakada,S.Miyashita and K.Watanabe: "Effects of a magnetic field on the nucleation and growth of protein crystals" Journal of Crystal Growth. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] E.Kashimoto,G.Sazaki,K.Hasegawa,T.Nakada,S.Miyashita and H.Komatsu: "Study on the crystallization of concanavalin A" Journal of Crystal Growth. (発表予定).

    • Related Report
      1996 Annual Research Report
  • [Publications] E.Yoshida,G.Sazaki,T.Nakada,S.Miyashita,H.Komatsu and N.Miimura: "Morphodrom of ferritin crystal" Journal of Crystal Growth. (発表予定).

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Sakuma,S.Miyashita,G.Sazaki,T.Nakada and H.Komatsu: "Motion of macro-steps induced on the surface of a lysozyme crystal" Journal of Crystal Growth. (発表予定).

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Nakada,G.Sazaki,S.Miyashita and H.Komatsu: "In situ observation of growing surface of lysozyme crystal" Journal of Crystal Growth. (発表予定).

    • Related Report
      1996 Annual Research Report
  • [Publications] A.Yanagiya,G.Sazaki,T.Nakada,S.Miyashita and H.Komatsu: "Effect of a magnetic field on the orientation of lysozyme crystal" Journal of Crystal Growth. (発表予定).

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Yanagiya,G.Sazaki,T.Nakada,S.Miyashita and H.Komatsu: "Effect of a magnetic field on the nucleation of lysozyme crystal" Journal of Crystal Growth. (発表予定).

    • Related Report
      1996 Annual Research Report
  • [Publications] B.R.Thomas,P.G.Vekilov and F.Rosenberger: "Heterogeneity determination and purification of commercial hen egg white lysozyme." Acta Cryst.D52. 776-784 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] P.Vekilov,L.A.Monaco,B.R.Thomas,V.Stojanoff and F.Rosenberger: "Repartitioning of NaCl and protein impurities in lysozyme crystallization." Acta Cryst.D52. 785-798 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] F.Rosenberger: "Protein crystallization" J. Crystal Growth. 166. 40-54 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Muschol and F.Rosenberger: "Lack of evidence for prenucleation aggregate formation in lysozyme crystal growth solutions." J. Crystal Growth. 167. 738-747 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] F.Rosenberger,P.G.Vekilov,M.Muschol and B.R.Thomas: "Nucleation and crystallization of globular proteins-what do we know and what is missing?" J. Crystal Growth. 168. 1-27 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] P.G.Vekilov,J.I.D.Alexander and F.Rosenberger: "Nonlinear response of layer growth dynamics in the mixed kinetics-bulk transport regime." Phys. Rev. E.54. 6650-6660 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] P.G.Vekilov,H.Lin and F.Rosenberger: "Unsteady crystal growth due to step-bunch cascading." Phys. Rev. E.55. 3202-3214 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Muschol and F.Rosenberger: "Liquid-liquid phase separation in supersaturated lysozyme solutions : Coupling to precipitate formation and crystallization." Biophys. J.(accepted).

    • Related Report
      1996 Annual Research Report
  • [Publications] F.Rosenberger,P.G.Vekilov,H.Lin and J.I.D.Alexander: "A rationale for system-dependent advantages and disadvantates of solution crystal growth at low gravity." Microgravity Sci. Technol.(accepted).

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Komatsu,S.Miyashita,T.Nakada,G.Sazaki and A.A.Chernov: ""An atomic level analysis of crystal growth mechanism in complex systems by means of nano-optical microscopy and AFM" in Advances in crystal growth mechanisms" Elsevier,Amsterdam, (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Nakada,S.Miyashita,G.Sazaki,H.Komatsu and A.A.Chernov: "Atomic force microscopic study of subsurface ordering and structural transforms in n-alcohol on mica and graphite" Jpn. J. Appl. Phys.35. L52-L55 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Kurihara,S.Miyashita,G.Sazaki,T.Nakada,Y.Suzuki and H.Komatsu: "Interferometric study on crystal growth of tetragonal lysozyme crystal." J. Crystal Growth. (印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] Gen Sazaki,K.Kurihara,T.Nakada,S.Miyashita and H.Komatsu: "A novel approach to the solubility measurement of protein crystals by two-beam interferometry" J. Crystal Growth. (投稿中).

    • Related Report
      1995 Annual Research Report
  • [Publications] E.Kashimoto,G.Sazaki,T.Nakada,S.Miyashita and H.Komatsu: "Stydy on the crystallization of concanavalin A" J. Crystal Growth. (発表予定).

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Sakuma,S.Miyashita,G.Sazaki,T.Nakada and H.Komatsu: "Motion of macro-steps induced on the surface of a lysozyme crystal" J. Crystal Growth. (発表予定).

    • Related Report
      1995 Annual Research Report
  • [Publications] P.G.Vekilov,M.Ataka,and T.Katsura: "Growth processes of protein crystals revealed by laser Michelson interferometry investigation" Acta Crystallographica D. 51. 207-219 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Martin Muschol and Franz Rosenberger: "Interactions in under-and supersaturated lysozyme solutions-static and dynamic light scattering results" J. Chem. Phys.

    • Related Report
      1995 Annual Research Report
  • [Publications] A.A.Chernov and H.Komatsu,(eds.J.P.van der Eerden and O.S.L.Bruinsma): "“Topics in Crystal Grwoth Kinetics."in “Science and Technology of Crystal Growth"" Kluwer Academic Publishers, 14 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.A.Chernov and H.Komatsu,(eds.J.P.van der Eerden and O.S.L.Bruinsma): "“Principles of crystal growth in protein crystallization."in “Science and Technology of Crystal Growth"" Kluwer Academic Publishers, 25 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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