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Development of Third Generation Biosensors

Research Project

Project/Area Number 07044129
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionUniversity of Tokyo

Principal Investigator

KARUBE Isao  Investigation and supervision of joint research, University of Tokyo, Professor, 先端科学技術研究センター, 教授 (50089827)

Co-Investigator(Kenkyū-buntansha) THOMAS Daniel  Development of water quality monitoring sensors, 酵素工学科, 教授
COMTAT Maurice  Development of gaseous materials biosensors, 物理・電気化学研究所, 教授
MARTY Jean-Louis  Development of multibiosensor for environmental monitoring, 応用複合領域研究所, 教授
COULET Pierre  Development of mulchichannel biosensors, 酵素遺伝子研究所, 教授
THEVENOT Daniel  Development of multibiosensor for environmental monitoring, 化学科, 教授
NOMURA Yoko  Development of multibiosensor for environmental monitoring, 特別研究員
SASAKI Satoshi  Development of gaseous materials biosensors, University of Tokyo, 先端科学技術研究センター, 助手 (70262110)
YANO Kazuyoshi  Development of water quality monitoring sensors, University of Tokyo, 先端科学技術研究センター, 助手 (40262109)
IKEBUKURO Kazunori  Development of mulchichannel biosensors, University of Tokyo, 先端科学技術研究センター, 講師 (70251494)
THOMAS Danie  コンピエーヌ工科大学, 酵素工学科, 教授
澤田 慎矢  日本学術振興会, 特別研究員
六車 仁志  日本学術振興会, 特別研究員
増地 矢恵子  東京大学, 先端科学技術研究センター, 客員助教授 (70270907)
松川 律子  東京大学, 先端科学技術研究センター, 客員助教授 (70112943)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 1996: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1995: ¥5,800,000 (Direct Cost: ¥5,800,000)
Keywordscyanide / phosphate / artificial diamond / genetic algorism / マルチバイオセンサー / 水質モニタ用センサー / 環境計測
Research Abstract

Development of multibiosensor for environmetal monitoring
Biosensor for cyanide detectionusing resparatory change of the microbe immobilized on the surface of oxygen electrode. This was focused on the future application of river water monitoring. Evaluation of the sensor developed were performed and the comparison woth conventional method was done. According to the discussion with Prof. Marty's group, who has a highly sofisticated technique on environmental monitoring, the sensor seemed to be very useful.
Development of multichannel biosensors
Several efforts were tried to have the higher sensitivity of the phosphate sensor using chemiluminescence. Research group of Prof. Coulet has already worked with chemiluminescence using luminol and their advice worked well for our purpose.
Development of gaseous materials biosensors
Electrophotochemical approach towards the detection of gaseous materials such as cyanide was concluded as effective for the purpose. The use of artificial diamond electrode for biosensor seemed also effective because of its wide potential window without being affected by water.
Development of water quality monitoring sensors
The use of catalytic antibody for a new biosensor seemed very interesting, and the preparation of bio-functional molecules using computer-aided method such as genetic algorism also seemed effective.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] I.Karube, Y.Nomura, Y.Arikawa: "Biosensor for environmental control" Trends Anal.Chem.14. 295-299 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kenji Hayashi, Satoshi Sasaki, Kazunori Ikebukuro, Isao Karube: "Highly sensitive chemiluminescence flow injection analysis system using microbial peroxydase and a photodiode detector" Anal.Chim.Acta.329. 127-134 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jeong Im Lee, Isao Karube: "Development of a biosensor for gaseous cyanide in solution" Biosensors & Bioelectronics. 11. 1147-1154 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] I.Karube,Y.Nomura,Y.Arikawa: "Biosensor for environmental control" Trends Anal Chem.14. 295-299 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kenji Hayashi,Satoshi Sasaki,Kazunori Ikebukuro,Isao Karube: "Highly sensitive chemiluminescence flow injection analysis system using microbial peroxydase and a photodiode detector" Anal.Chim.Acta.329. 127-134 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Jeong Im Lee,Isao Karube: "Development of a biosensor for gaseous cyanide in solution" Biosensors & Bioelectronics. 11. 1147-1154 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kazunori Ikebukuro,Isao Karube,et al.: "Phosphate sensing system using pyruvate oxydase and chemiluminescence detection" Biosensors and Bioelectronics. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazunori Ikebukuro,Isao Karube et al.: "A novel biosensor system for the determination of phosphate" Journal of Biotechnology. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazunori Ikebukuro,Isao Karube et al.: "A novel biosensor system for cyanide based on the chemiluminescence reaction" Analytica Chimica Acta. (in press).

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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