Genetic Production of Lipid-antibody for Novel Sensing
| Project/Area Number |
07044134
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
生物・生体工学
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
AIZAWA Masuo Tokyo Institute of Technology, Professor, 生命理工学部, 教授 (00016742)
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| Co-Investigator(Kenkyū-buntansha) |
KEINANEN Kari VTT Biotechnology and Food Research Senior Researcher, 生物工学研究所, 主任研究員
HARUYAMA Tetsuya Tokyo Institute of Technology Assistant Professor, 生命理工学部, 助手 (30251656)
KOBATAKE Eiry Tokyo Institute of Technology Associate Professor, 生命理工学部, 助教授 (00225484)
KELN●NEN Kar フィンランド工学研究センター(VTT), 生物工学研究所, 主任研究員
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 1997: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1996: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1995: ¥3,300,000 (Direct Cost: ¥3,300,000)
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| Keywords | lipid-tagged antibody / immunoliposome / immunosensing / 2-phenyoxazolone / QCM / AFM / genetic engineering / 配列制御分子膜 / フェニルオキサゾロン / タンパク質工学 / バイオセンシング |
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| Research Abstract |
The single-chain antibody against 2-phynyoxazolone was modified with lipid molecules at its amino-terminus by genetic engineering. The lipid-tagged antibody. Prepared by this method retains its antigen binding property. The engineered-lipid-tagged antibody molecules were incorporated into liposomes consisting of phosphatidylcholine, and immunoliposomes were constructed. We have developed two kinds of immunosensing system using the immunoliposomes. One system was based on quartz crystal microbalance (QCM). The immunoliposomes were adsorbed to the antigen-immobilized surface of a crystal plate with various concentrations of soluble antigen molecule. The frequency change was observed by injection of the mixture of the immunoliposomes and oxazolone in a concentration dependent manner. The other sensing system was based on atomic force microscopy (AFM). The immunoliposomes bound on the antigen-adsorbed mica surgface were directly observed by AFM.The numbers of observed immunoliposomes depend on the concentration of coexisting soluble antigen molecule. Novel immunosensing systems were realized through the use of immunoliposomes containing genetically prepared lipid-tagged antibody.
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Report
(4 results)
Research Products
(10 results)
