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Joint study on the mechanisms of reproduction in crustacea

Research Project

Project/Area Number 07044181
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionOcean Research Institute, University of Tokyo

Principal Investigator

NAGASAWA Hiromichi  Ocean Research Institute, University of Tokyo, Professor, 海洋研究所, 教授 (60134508)

Co-Investigator(Kenkyū-buntansha) FUNKENSTEIN Bruria  Israel Oceanographic & Limnological Inst., Researcher, 研究員
TIETZ-DEVIR Alisa  Tel Aviv University, Prof., 生命科学科, 教授
LUBZENS Esther  Israel Oceanographic & Limnological Inst., Assoc.Pfor., 助教授
AIDA Katsumi  Graduate Shool of Agr.Biosci.Univ.of Tokyo, Prof., 大学院・農学生命科学研究科, 教授 (50012034)
BRURIA Funke  イスラエル海洋陸水研究所, 研究員
ALISA Tietzー  テルアビブ大学, 生命科学科, 教授
ESTHER Lubze  イスラエル海洋陸水研究所, 助教授
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1996: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsCrustacea / Penaeus japonicus / sinus gland / neuropeptide / vitellogenesis / vitellogenesis-inhibiting hormone / peptide hormone / ナイナス腺
Research Abstract

The endocrinological mechanisms of reproduction, especially vitellogenesis, in crustaceans have been investigated. It has been estimated that neuropeptides in the sinus gland inhibit vitellogenesis. We first extracted and purified seven structurally related neuropeptides from the sinus gland, and determined their whole amino acid sequences. They were designated as Pej-SGP-I--VII.All these peptides except for IV showed hyperglycemic acitivity, while IV had an activity to inhibit ecdysone release by Y-organ in in vitro culture system. These peptides were examined for vitellogenesis-inhibiting activity by using in vitro culture of pieces of developing ovary from the prawn Penaeus semisulcatus. The effects of these peptides on protein synthesis was examined by incorporation of ^<35>S-methionine into proteins in the ovarian pieces. The results showed that all the peptides except for IV inhibited protein synthesis. The inhibition was not confined to a specific protein but to all proteins. Ex … More periments of incorporation of ^3H-uridine showed that the inhibition occurred at the transcription level. By contrast, Pej-SGP-IV,red pigement concentrating hormone, pigment-dispersing hormone, 20-hydroxyecdysone or methyl farnesoate did not show any inhibiting or stimulating activity, suggesting the specific inhibition of six peptides.
There has been no information about the structure of yolk protein vitellin or its precursor vitellogenin in crustaceans. First, vitellin was purified from the ovary of P.semisulcatus. By SDS-PAGE,it was separated into mainly 4 subunits, 160,120,96, and 80kDa. Blotted subunit proteins were sequenced and it was found that the N-terminal sequence of larger two were identical and that of smaller two were also identical. But the sequences between the two groups were different. Internal sequences were analyzed after digestion of native vitellin with lysyl endopeptidase and sebsequent separation of the digested fragments. Thus, we have obtained a lot of partial sequences. Using these peptide sequence information, we are now cloning cDNA encoding vitellogenin to know its full sequence. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Wei-Jun Yang et al.: "Amino acid sequences of a hyperglycemic hormone and its related peptides from the kuruma prawn,Penaeus japonicus" Aquaculture. 135. 202-212 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wei-Jun Yang et al.: "Amino acid sequence of a peptide with molt-inhibiting activity from the kuruma prawn Penaeus japonicus" Peptides. 17. 197-202 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiromichi Nagasawa et al.: "Isolation an amino acid Sequence of a molt-inhibiting hormone from the American crayfish,Procambarus clarkii" Biosci.Biotech.Biochem.60. 554-556 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiko Terauchi et al.: "Identification of a molt-inhibiting hormone from the sinus gland of the crayfish,Procambarus clarkii" Zool.Sci.13. 295-298 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsuyoshi Ohira et al.: "Cloning and sequence analysis of a cDNA encoding a crustacean hyperglycemic hor-mone from the kuruma prawn Penaeus japonicus" Mol.Mar.Biol.Biotech.(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wei-Jun Yang et al.: "Amino acid sequences and activities of muttiple hyperglycemic hormones from the kuruma prawn,Penaeus japonicus" Peptides. (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 長澤寛道(分担執筆): "昆虫の生化学・分子生物学" 名古屋大学出版会, 498 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wei-Jun Yang et al.: "Amino acid sequences of a hyperglycemic hormone and its related peptides from the kuruma prawn, Penaeus japonicus" Aquaculture. 135. 202-212 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wei-Jun Yang et al.: "Amino acid sequence of a peptide with molt-inhibiting activity from the kuruma prawn Penaeus japonicus" Peptides. 17. 197-202 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiromichi Nagasawa: "Isolation and amino acid sequence of a molt-inhibiting hormone from the American Crayfish, Procambarus clarkii" Biosci.Biotech.Biochem.60. 554-556 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiko Terauchi et al.: "Identification of a molt-inhibiting hormone from the sinus gland of the crayfish, Procambarus clarkii" Zool.Sci.13. 295-298 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsuyoshi Ohira et al.: "Cloning and sequence analysis of a cDNA encoding a crustacean hyperglycemic hormone from the Kuruma prawn Penaeus japonicus" Mol.Mar.Biol.Biotech.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wei-Jun Yang et al.: "Aminoacid sequences and activities of multiple hyper-glycemic hormones from the Kuruma prawn, Penaeus japonicus" Peptides. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiromichi Nagasawa: Nagoya University Press. Biochemistry and Molecular Biology in Insects, 219-232 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wei-Jun Yang et al.: "Amino acid sequence of a paptide with molt-inhibiting activity from the kuruma prawn penaeus japonicus" Peptides. 17. 197-202 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hiromichi Nagasawa et al.: "Isolation and amino acid sequence of a molt-inhibiting hormone from the American crayfish,Procambarus clarkii" Biosci.Biotech.Biochem.60. 554-556 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akiko Terauchi et al: "Identification of a molt-inhibiting hormone from the sinus gland of the crayfish,Procambarus clarikii" Zool,Sci.13. 295-298 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsuyoshi Ohira et al.: "Cloning and sequence analysis of a cDNA encoding a crustacean hyperglycemic hormone from the kuruma prawn penaeus japonicus" Mol.Mar.Biol.Biotech.印刷中. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Wei-Jun Yang et al.: "Amino acid sequences and activities of multiple hyperglycemic hormones from the kuruma prawn,Penaeus japonicus" Peptides. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Wei-jun Yang,Katsumi Aida and Hiromichi Nagasawa: "Amino acid sequences of a hyperglycaemic hormone and its related peptides from the kuruma prawn,penaeus japonicus" Aquaculture. 135. 205-212 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wei-jun Yang,Katsumi Aida,Akiko Terauchi,Haruyuki Sonobe and Hiromichi Nagasawa: "Amino acid sequences of a peptide with moit-inhibiting activity from the kuruma prawn penaeus japonicus" Peptides. (印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiromichi Nagasawa,Wei-jun Yang,Hidetsugu Shimizu,Katsumi Aida,Hibiki Tsutsumi,Akiko Terauchi and Haruyuki Sonode: "Isolation and amino acid sequencemof amolt-i inhibiting hormone from the American crayfish,procam barus clarkii" Biosci・Biotech. Biochem.(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Akiko Terauchi,Hibiki Tsutsumi,Wei-jun Yang,Kautumi,Aida,Hiromi Nagasawa and Haruyuki Sonobe: "Isolation of a molt-inhibiting hormone from thr sinus gland of the crayfish,Procambarus clarkii" Zool.Sci.(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 長澤寛道: "昆虫の生化学・分子生物学" 名古屋大学出版会, 498 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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