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Regulation mechanism of the electron transport in chloroplasts

Research Project

Project/Area Number 07044188
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionKanazawa University

Principal Investigator

WADA Keishiro  Dept.of Biol., Fac.of Sci., Kanazawa Univ. professor, 理学部, 教授 (70028174)

Co-Investigator(Kenkyū-buntansha) GOLBECK J.h.  Dept.of Biochem., Univ.of Nebraska (Lincoln), 生化学, 教授
BLANKENSHIP R.e  Dept.of Chem./Biochem., Arizona State Univ. (Tempe), 生化学・化学, 教授
BUCHANAN B.b.  Dept.of Plant Biol., Univ.of California (Berkeley), 植物生物学, 教授
TAKAHASHI Yasuhiro  Dept.of Biol., Fac.of Sci., Osaka Univ., 理学部, 助手 (10154874)
OHOKA Hirozo  Dept.of Biol., Fac.of Sci., Osaka Univ., 理学部, 助手 (30201966)
MATSUBARA Hiroshi  Dept.of Biochem., Fac.of Sci., Okayama Science Univ., 理学部, 教授 (00028242)
HOSHINA Satoshi  Dept.of Biol., Fac.of Sci., Kanazawa Univ., 理学部, 助教授 (50019486)
Project Period (FY) 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordschloroplasts / plastids / photosystem complex / green sulfur bacteria / electron tranport system / FNR / ferredoxins / thioredoxin
Research Abstract

Regulation mechanism of the electron transport in chloroplasts and the related platids was investigated by the group including three American scientists who are experts in each field. Experiments were planed by focussing on
1) regulation mechanism of electron transport in photosystem I core complex,
2) regulation mechanism of electron transport to NADP^+ or from NADP^+ around ferredoxin in chloroplasts and plastids of non-photosynthetic tissues,
3) regulation mechanism of several proteins by thiol-disulfide exchange reaction which is mediated by thioredoxins,
4) redox regulation by thioredoxin system.
Each electron transferring components in Fe-S type reaction centers from chloroplasts from higher plants, green sulfur bacteria and primitive bacterium Heliobacter mobilis were compared in the aspect of structure and function.
With PCR method cDNA of ferredoxin-NADP oxidoreductase mainly expressed in non-photosynthetic tissues was cloned and compared with other cDNA sequences of FNR from rice and maize.
The new function of thioredoxin h was characterized and the allergenicity in food proteins was clarified to be reduced by thioredoxin.

Report

(1 results)
  • 1995 Final Research Report Summary
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Kamide,K.,Sakai,H.,Aoki,K.,Sanada,Y.,Wada,K.,Green,L.S.,Yee,B.C.and Buchanan,B.B.: "Amino acid sequences of heterotrophic and photosynthetic ferredoxins ferredoxin from the tomato plant." Photosynthesis Research. 46. 301-308 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sanada,Y.,Ueda,H.,Kuribayashi,K.Andoh,T.,Hayashi,F.,Tamai,N.,and Wada,K.: "Novel light-dark change of proline levels in Halophytes (Mesembryanthemum crystallinum) and Glycophytes.." Plant and Cell Physiology. 36(6). 965-970 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Aoki,K.and Wada,K.: "Spacial and temporal distribution of ferredoxin isoproteins in tomato (Lycopersicon esculentum L.) fruit." Plant Physiology. (submitted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Zhu,Y.,Ramakrishna,B.L.,van Noort,P.I.,Blankenship,R.E.: "Microscopic and spectroscopic studies of untreated and hexanol-treated chlorosomes from Chloroflexus....." Biochimica et Biophisica Acta. 1232. 197-207 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hastings,G.,Hoshina,S.,Webber,A.N.and Blankenship,R.E.: "Universality of energy and electron trnasfer processes in photosystem I" Biochemistry. 34. 05512-05522 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hastings,G.,Reed,L.J.,Lin,S.and Blankenship,R.E.: "Excited state dynamics in photosystem I : Effects of detergent and excitation wavelength." Biophysical Journal. 69. 2044-2055 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Lin,S.,Chiou,H-C.and Blankenship,R.E.: "Secondary electron transfer processes in membranes of Heliobacillu mobilis." Biochemistry. 34. 12761-12767 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Lee,W-Y.,Brume,D.C.,LoBrutto,R.and Blankenship,R.E.: "Isolation,characterization and primary structure of rubredoxin from the photosynthetic bacterium..." Archives of Biochemistry and Biophysics. 318. 80-88 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Vassiliev,I.R.,Jung,Y-S.,Smart,L.B.,Schluts,R.,McIntosh,L.and Golbeck,J.H.: "A mixed-ligand iron-sulfur cluster (C556S_<PsaB> or C565S_<PsaB>) in the Fxbinding site leads to a decreased..." Biophysical Journal. 69. 1544-1553 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Buchanan,B.B.,Lozano,R.,Yee,B.C.,Frick,O.L.and Ermel,R.: "Reduction of allergenicity in foods by thioredoxin." Ann.Allergy,Asthma and Immunology. 74. 112- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Garbisu,C.,Gonzalez,S.,Yang,W.-H.,Yee,B.C.,Carlson,D.L.Yee,A.,Smith,N.R.,Otero,R.,Buchanan: "Physiological mechanisms regulating the conversion of selenite to elemental selenium by B.subtilis." BioFactors. 5. 29-37 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wong,J.H.,Kobrehel,K.and Buchanan,B.B.: "Thioredoxin and seed proteins." Methods in Enzymology. 252. 228-240 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Lozano,R.M.,Wong,J.H.,Yee,B.C.,Peters,A.,Kobrehel,K.and Buchanan,B.B.: "New evidence for a role for thioredoxin h in germination and seedling development." Planta. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Besse,I.,Wong,J.H.,Kobrehel,K.Buchanan,B.B.: "Thiocalsin : a thioredoxin-linked substrate specific protease dependent on calcium" National Academy of Science,USA. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kamide et al.: "Amino acid sequences of heterotrophic and photosynthetic ferredoxins from the tomato plant." Photosynthesis Research. 46. 301-308 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Sanada et al.: "Novel light-dark change of proline levels in Halophyte (Mesembryanthemum crystallinum L.)..." Plant and Cell Physiology. 36. 965-970 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Aoki and K.Wada: "Spacial and temporal distribution of ferredoxin isoproteins in tomato (L.esculentum L.) fruit." Plant Physiology (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Zhu, et al.: "Microscopic and spectroscopic studies of untreated and hexanol-treated chlorosomes from Chloroflexus..." Biochimca et Biophysica Acta. 1232. 197-207 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] G.Hastings et al.: "Universality of energy and electron transfer processes in photosystem I." Biochemistry. 34. 15512-15522 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] G.Hastings et al.: "Excited state dynamics in photosystem I : Effects of detergent and excitation wavelength." Biophysical Journal. 69. 2044-2055 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Lin et al.: "Secondary electron transfer processes in membranes of Heliobacillus mobilis." Biochemistry. 34. 12761-12767 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] W.-Y.Lee et al.: "Isolation, characterization and primary structure of rubredoxin from the photosynthetic bacterium...." Archives of Biochemistry and Biophysics. 314. 80-88 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] I.R.Vassiliev et al.: "A mixed-ligand iron-sulfur cluster (C556S_<psaB> or C565S_<psaB>) in the Fx-binding site leads to a dec..." Biophysical Journal. 69. 1544-1553 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] B.B.Buchanan et al.: "Reduction of allergenicity in foods by thioredoxin" Annalus Allergy, asthma and Immunology. 74. 112- (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] C.Garbisu et al.: "physiological mechanisms regulating the conversion of selenite to elemental selemium by B.subtilis." BioFactors. 5. 29-37 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] J.H.Wong et al.: "Thioredoxin and seed proteins." Methods in Enzymology. 252. 228-240 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] R.M.Lozano et al.: "New evidence for a role for thioredoxin h in germination and seedling development." Planta (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] I.Besse et al.: "Thiocalsin : a thioredoxin-linked substrate specific protease dependent on calcium." National Academy of Science, USA (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary

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Published: 1995-04-01   Modified: 2016-04-21  

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