| Project/Area Number |
07044222
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | GUNMA UNIVERSITY |
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Principal Investigator |
MINEGISHI Takashi Gunma University School of Medicine, Dept.of OB/GYN,Assistant Professor, 医学部, 講師 (00209842)
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| Co-Investigator(Kenkyū-buntansha) |
LEUNG Peter C.K. University of British Colombia, Dept.of OB/GYN,Professor, University of British Col, Prefessor
ABE Yumiko Gunma University School of Medicine, Dept.of OB/GYN,, 医学部, 教務員 (70261857)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | LH / hCG receptor / splicimg / expression / co7 cell / cAMP / CHO cell / cAMP / カルシウム / 卵胞 / アクチビン / FSHレセプター |
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| Research Abstract |
Cloning of the human LH/HCG receptor cDNA previously revealed the existence of two alternative forms of the receptor differing by the presence (HLH-Ra) and absence (HLH-Rb) of 62 amino acids encoded by exon 9. We have studied functional significance of these receptor isoforms and have confirmed that they are generated by alternative splicing. A reverse transcription-polymerase chain reaction amplification was used to detect different isoforms of LH receptor mRNAs in ovary and placenta. The expression of the two mRNA forms of LH/HCG receptor were detected in ovary, and at very low concentrations in placenta. Treatment with HCG caused a dosedependent increase in cAMP production with an initial response evident at -1 ng/ml HCG in COS-7 cells expressing HLH-Ra. However, a complete loss of signal transduction was found in cells transfected with the truncated HLH-Rb.
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