| Project/Area Number |
07044241
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Fukui Medical School |
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Principal Investigator |
KOTSUJI Fumikazu Fukui Medical School Professor, 医学部, 教授 (50153573)
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| Co-Investigator(Kenkyū-buntansha) |
TSANG Benjamin K Fukui Medical School Professor, University of Ottawa Repro, 教授
AMSTERDAM Abraham Fukui Medical School Professor, The Weizmann I, 教授
HOSOKAWA Kumiko Fukui Medical School Instructor, 医学部, 助手 (60199495)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1996: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1995: ¥3,600,000 (Direct Cost: ¥3,600,000)
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| Keywords | Granulosa Cell / Theca Cell / Cellular Interaction / Oncogene / Steroidogenesis / Apoptosis / meiosis-arrest / P450scc / アドレノドキシン / p53 / 細胞外マトリックス(ECM) / Granurosa Cell / Theca Cell / Cellular Interaction / Apoptosis / Progesterone |
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| Research Abstract |
Establishment of Steroidogenic Granulosa Cell Lines Originating from Women
The life span of granulosa cells obtained from human ovarian follicle is very short. We established new granulosa cell lines by triple transfection of primary cells with SV40 DNA,Ha-RAS gene and p53 gene. These stable cell lines proliferated rapidly and showed steroidogenic activity. cAMP stimulate progesterone production. Shifting the temperature of growth from 37C to 32C induced programd cell death. This newly established granulosa cell lines can serve as a useful tool for the study of the molecular and cellular mechanisms of inducible steroidogenesis in immortalized human granulosa cells.
Effects of Thecal Cells on Apoptosis of Granulosa cells
Using the co-culture system in which granulosa and thecal cells were maintained on the opposite surface of a collagen membrane, effects of thecal cells on apoptosis of granulosa cells was investigated. Hen theca cells stimulated hen granulosa cell apoptosis. However, such
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effect of theca cells was not detected when follicular cells were collected from bovine ovaries.
Effects of Thecal Cells on Granulosa Cell Differentiation
The possible role of thecal cells in the control of the hormonal producing activity of granulosa cells was investigated. Thecal cells reduced estradiol, inhibin and progesterone production by granulosa cells from early stage of follicular development, while augmented those by cells at late developmental stage. These results demonstrate that the thecal cells play an important role in the control of granulosa cell differentiation during ovarian follicular development.
Effects of Androstenedione and Estradiol on the Meiosis-Arresting Activity of the Granulosa Cells Cultured Together with Thecal Cells
The present study explored how the meiosis-arresting activity of bovine granulosa cells is controlled by androstenedione and estradiol of the follicular fluid. This study showed that androstenedione controls the meiosis-arresting activity of the granulosa cells biphasically depending on its dose ; growing follicle fluid level of androstenedione augments the meiosis-arresting activity of granulosa cells, while atretic follicle level of androstenedione suppresses it. Estradiol does not such effect as that of androstenedione. Less
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