| Project/Area Number |
07044244
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Res.Inst.of Environ.Med., Nagoya Univ. |
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Principal Investigator |
KODAMA Itsuo Res.Inst.of Environ.Med., Nagoya Univ., Prof., 環境医学研究所, 教授 (30124720)
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| Co-Investigator(Kenkyū-buntansha) |
BOYATT Mark R Dept.of Physiol., Univ.of Leeds, UK,Prof., 生理学, 教授
HONJO Haruo Res.Inst.of Environ.Med., Nagoya Univ., Res.Assoc., 環境医学研究所, 助手 (70262912)
TOYAMA Junji Res.Inst.of Environ.Med., Nagoya Univ., Prof., 環境医学研究所, 教授 (20023658)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | sinoatrial node / spontaneous activity / action potential / ion channel / electrophysiology / heart / 自発活動電位 / イオン電流 / パッチクランプ / 4-aminopyridine / 細胞歩調取り機能 / 電気生理学的多様性 / 細胞内イオン動態 |
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| Research Abstract |
The SA node is an inhomogeneous structure in terms of structure, properties and function. We have investigated the possible regional deferences in the role of various membrane currents in the pacemaker activity in rabbit SA node preparations. Block of L type Ca^<2+> current (I_<Ca>) by 2muM nifedipine abolished spontaneous action potentials in the center, but not in the periphery, in the intact SA node and small ball-like specimens of the SA node. In contrast, block of Na^+ current (I_<Na>) by 20muM TTX had no effect on the electrical activity in small balls from the center, but it slowed the spontaneous acivity in balls from the periphery of the SA node. An application of 2 mM Cs^+,1muM UL-SF 49 or 3muM ZD7288, which has been reported to block hyperpolarization-activated current (I_f), decreased pacemaker slope and spontaneous rate of small balls of the SA node. The decrease was largest in balls from the periphery and least in balls from the center. These results suggest that I_<Ca> Plays an obligatory role in pacemaking in the center, whereas I_<Na> Plays a major role in pacemaking in the periphery, and that the contribution of I_f to spontaneous activity is greater in the periphery than in the center of the SA node. 4-aminopyridine (4-AP), which is known to block a population of K^+ current, caused a marked prolongation of action potential and a slowing of spontaneous rate in the periphery, but it accelerated spontaneous activity in the center of the SA node. This suggests that 4-AP-sensitive currents are also involved in the heterogeneity of the electrical activity within the SA node.
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