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プロテインC凝固制御系異常症の分子病態学的研究

Research Project

Project/Area Number 07044246
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionMie University

Principal Investigator

鈴木 宏治  三重大学, 医学部, 教授 (70077808)

Co-Investigator(Kenkyū-buntansha) STENFLO Joha  ルンド大学, 医学部, 教授
DAHLBACK Bjo  ルンド大学, 医学部, 教授
林 辰弥  三重大学, 医学部, 助手 (00242959)
武谷 浩之  三重大学, 医学部, 助手 (60222105)
井戸 正流  三重大学, 医学部, 講師 (90167263)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥8,200,000 (Direct Cost: ¥8,200,000)
Fiscal Year 1996: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1995: ¥4,600,000 (Direct Cost: ¥4,600,000)
Keywords先天性血栓性素因 / プロテインC欠損症 / プロテインS欠損症 / APCレジスタンス / トロンボモジュリン欠損症 / プロテインC受容体欠損症 / プロテインC凝固制御系 / 血液凝固制御系異常症 / プロテインC異常症 / プロテインS異常症 / 第V因子異常症 / 血液凝固制御 / トロンボモジュリン
Research Abstract

血液中や血管内壁上には複数の血液凝固制御機構が存在し、血液の流動性を維持している。なかでもプロテインC凝固制御系は最も重要であり、プロテインC凝固制御系因子の先天性欠損症は高頻度に血栓塞栓症を来すことが知られている。1993年スウェーデン・ルンド大学のDahlback教授らによって発見された活性化プロテインC不応症(APCレジスタンス)は、APCの基質である凝固第V因子のAPC切断部位(Arg506-Ser507)のアミノ酸変異(Arg506→Gln)を原因とし、その発生頻度は白人で特に高く、血栓症患者全体の20〜50%にも及ぶ。APCレジスタンスをはじめとしてプロテインC凝固制御系因子欠損症の分子遺伝学的研究は発症頻度の高い北欧の大学で最も進んでいるため、申請者らは、本科研費の補助の下、ルンド大学のプロテインC発見者のStenflo教授、APCレジスタンス発見者のDahlback教授との国際共同研究として実施した。昨年度来、我々は三重大学をはじめとする国内の大学病院の血栓症患者約200名についてAPCレジスタンス症の発生頻度を解析し、患者の約3%にAPC添加凝固時間の短縮(APCレジスタンス症状)を認めたが、いずれの患者遺伝子にも欧米の患者にみられるArg506→Gln変異は認めなかった。また、その他のプロテインC凝固制御系因子欠損症についても分子病態学的解析を行い、成果を上げることができた。本年度2月に鈴木はルンド大学を訪問し、大いに研究を進めることが出来たが、11月に来日の予定であったDahlback教授は彼の都合が付かないため、やむを得ず次年度の5月に来日して戴くことになった。現在、Dahlback教授らとはE-mail等で情報交換を行い、我国で未発見のトロンボモジュリン欠損症および世界でも未だ報告の無いプロテインC受容体欠損症を発見するための共同研究を行っている。

Report

(2 results)
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Gabazza EC,Takeya H,Suzuki K,et al.: "Acquired acitvated protein C resistance in patients with lupus anticoagulant and essential thrombocythemia." Clin.Appl.Thromb.Hemostas. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Gandrille S,Borgel D,Suzuki K,et al.: "Protein S deficiency:A database of mutations-1996." Thromb.Haemostas.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Deguchi H,Takeya H,Suzuki K,et al.: "Kringle 1 domain of prothrombin interacts with factor Va during the assembly of the prothrombinase complex." Biochem.J.321. 729-735 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Gabazza EC,Takeya H,Suzuki K,et al.: "Protein C activation in NIDDM patients." Diabetologia. 39. 1455-1461 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mizutani H,Hayashi T,Suzuki K,et al.: "Functional thrombomodulin expression on epithelial skin tumours as a differential marker for suprabasal keratinocytes." Brit.J.Dermatol.135. 187-193 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ido M,Hayashi T,Suzuki K,et al.: "Ca_<2+>-dependent activation of the 33-kDa protein kinase transmits thrombin receptor signals in human platelets." Thromb.Haemostas.76. 439-443 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ido M,Hayashi T,Suzuki K,et al.: "Prenatal diagnosis of compound heterozygous deficiency of protein C by direct detection of the mutation sites." Thromb.Haemostas.76. 275-281 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sun J,Yamaguchi M,Suzuki K,et al.: "Purification,characterization and cDNA cloning of a novel anticoagulant heme protein(rhodhemin)from salivary glands of blood sucking bug,Rhodnius prolixus." Thromb.Haemostas.75. 573-577 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kise H,Nishioka J,Suzuki K,et al.: "Characterization of semenogelin II,and its molecular interaction with prostate specific antigen and protein C inhibitor." Eur,J.Biochem.238. 88-96 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nishioka J,Ido M,Suzuki K,et al.: "The Gla^<26> residue of protein C is required for the binding of protein C to thrombomodulin and endothelial cell protein C receptor,but not to protein S and factor Va." Thromb.Haemostas.75. 275-282 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mizutani H,Hayashi T,Suzuki K,et al.: "Increased endothelial and epidermal thrombomodulin cxpression and plasma thrombomodulin level in progressive systemic sclerosis." Acta Med.Okayama. 50. 293-297 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mori T,Takeya H,Suzuki K,et al.: "β2-Glycoprotein I modulates anticoagulant activity of activated protein C on phospholipids." Thromb.Haemostas.75. 49-55 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tamei H,Hoshino T,Suzuki K,et al.: "One-step sandwich enzyme immunoassay for human C4b-binding protein(C4BP)and protein S-C4BP complex using monoclonal antiodies." Clin.Chim.Acta. 234. 115-125 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohnishi K,Nagata K,Suzuki K,et al.: "Stimulation of Sky receptor tyrosine kinase by the product of growth-arrest-specific gene 6." J.Biol.Chem.270. 22681-22684 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hayashi T,Nishioka J & Suzuki K.: "Molecular mechanism of dysfunctional protein S-Tokushima(Lys^<155>→Glu)in the regulation of blood coagulation." Biochim.Biophys.Acta.1272. 159-167 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Iino M,Takeya H & Suzuki K,et al.: "Characterization of factor Xa binding to fibrinogen/fibrin derivatives.Comparative study with thrombin binding." Eur.J.Biochem.232. 90-97 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuda H,Hayashi T & Suzuki K.: "Molecular cloning and functional characterization of rat plasma protein S." J.Biochem.117. 374-383 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakagawa H,Takahashi N,Suzuki K,et al.: "A structural study of the oligosaccharide moeities of human coagulation factor X activation peptide." Glycoconjugate J.12. 173-181 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鈴木 宏治: "分子細胞生物学辞典" 東京化学同人(村松 正実 他編), 1040 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鈴木 宏治: "臨床検査 増刊 -血栓症と血小板凝固線溶系検査-(プロテインC、プロテインS、APC・PCI複合体)" 医学書院(猪狩 淳 他編), 292(13) (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鈴木 宏治: "臨床検査ガイド'96(プロテインC、プロテインS、APCレジスタンス、トロンボモジュリン)" 文光堂(Medical Practice編集委員会 編), 1061(8) (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki K.: "Molecular Basis of Thrombosis and Hemostasis(Protein C)" Marcel Dekker,Inc,New York(eds.Roberts HR & High KA), 688(31) (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki K.: "Molecular Basis of Thrombosis and Hemostasis(Protein S)" Marcel Dekker,Inc,New York(eds.Roberts HR & High KA), 688(19) (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鈴木 宏治: "図説 分子病態学" 中外医学社(一瀬白帝、鈴木宏治 編), 553 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mori T,Takeya H,Suzuki K,et al.: "β2-Glycoprotein I modulates anticoagulant activity of activated protein C on phospholipids." Thromb. Haemostas.75. 49-55 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ohnishi K,Nagata K,Suzuki K,et al.: "Stimulation of Sky receptor tyrosine Kinase by the product of growth-arrest-specific gene 6." J.Biol. Chem.270. 22681-22684 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hayashi T,Nishioka J & Suzuki K.: "Molecular mechanism of dysfunctional protein S-Tokushima (Lys^<155>→Glu)in the regulation of blood coagulation." Biochim. Biophys. Acta.1272. 159-167 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Iino M,Takeya H & Suzuki K,et al.: "Characterization of factor Xa binding to fibrinogen/fibrin derivatives. Comparative study with thrombin binding." Eur. J.Biochem.232. 90-97 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yasuda H,Hayashi T & Suzuki K.: "Molecular cloning and functional characterization of rat plasma protein S." J.Biochem.117. 374-383 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakagawa H,Takahashi N,Suzuki K,et al.: "A structural study of the oligosaccharide moeities of human coagulation factor X activation peptide." Glycoconjugate J.12. 173-181 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki K.: "Molecular Basis of Thrombosis and Hemostasis(Protein C)" Marcel Dekker,Inc,New York(eds. Roberts HR & High KA), 688(31) (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki K.: "Molecular Basis of Thrombosis and Hemostasis(Protein S)" Marcel Dekker,Inc,New York(eds. Roberts HR & High KA), 688(19) (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鈴木宏治: "遺伝子診断実践ガイド" 中外医学社(中井利昭 他 編), 485 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鈴木宏治: "図説分子病態学" 中外医学社(一瀬白帝、鈴木宏治 編), 553 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鈴木宏治: "臨床血栓止血学" 医歯薬出版(櫻川信男、池田康夫 編), 489 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鈴木宏治: "止血・血栓・線溶" 中外医学社(松田道夫、鈴木宏治 編), 500 (1994)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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