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Biological function of basement membrane

Research Project

Project/Area Number 07044268
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionOkayama University Medical School

Principal Investigator

NINOMIYA Yoshifumi  Okayama University Medical School, 医学部, 教授 (70126241)

Co-Investigator(Kenkyū-buntansha) オウ スーク・ポール  ハーバード大学, 医学部, 研究員
ウオルマン マット  ハーバード大学, 医学部, 研究員
アプラ スニール  ハーバード大学, 医学部, 研究員
MOMOTA Ryusuke  Okayama University Medical School, 医学部, 助手 (80263557)
OOHASHI Toshitaka  Okayama University Medical School, 医学部, 助手 (50194262)
オルセン ビヨン  ハーバード大学, 医学部, 教授
APTA Suneel  Harvard Medical School
OLSEN Bjorn  Harvard Medical School
OH Suk Paul  Harvard Medical School
WARMAN Matt  Harvard Medical School
スーク ポール・オウ  ハーバード大学, 医学部, 研究員
マット ウォルマン  ハーバード大学, 医学部, 研究員
スニール アプラ  ハーバード大学, 医学部, 研究員
ビヨン オルセン  ハーバード大学, 医学部, 教授
マット ウオルマン  ハーバード大学, 医学部, 研究員
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1996: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsgenomic library / monoclonal antibodies / homologous recombination / collagen genes / phynotypic change / knockout mouse / 遺伝子ライブラリ / cDNAライブラリー / IV型コラーゲン
Research Abstract

1) We have cloned many overlapping cDNAs coding for the mouse alpha6 (IV) collagen chain and determined primary structure of the alpha6 (IV) chain polypeptide.
2) We raised monoclonal antibodies against amino acid sequences from various parts of the mouse alpha6 (IV) collagen polypeptide chain and used them for the tissue distribution. The results indicated that the tissue distribution for the chain was similar to that of the human alpha6 (IV) collagen chain. Interestingly, the distribution of the paired genes ; col4a1/col4a2, col4a3/col4a4, and col4a5/col4a6 is quite synchronous except for the glomerular basement membranes.
3) We have some indication that shows alternative splicing in exon 31. Currently, we are characterizing this atypical splicing event in terms of tissue specific expression.
4) In order to create a knockout mice for col4a6, we made several constructs including the one where the exon 2 was taken out and the foreign gene, Neo^R gene was inserted instead. The construct was transfected into Stem cells by homologous recombination. Currently, we are characterizing knockout mice to detect any phenotypic changes in heterozygotes and homozygotes.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Sugihara K.: "Experimental Anti-GBM glomerular nephritis induced in rats by immunization with syhthetic peptides based on six a chains of human type IV collagen." J.Pathol.178. 352-358 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ljubimov A.V.: "Extracellular alterations in human corneas with bullous keratopathy." Invest.Ophthal mol.Vis.Sci.37. 997-1007 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Rauf S.: "Nephritogenicity and a-chain composition of NC1 fractions of type IV collagen from bovine renal basement membrane." Virchow Archiv. 428. 281-288 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hino S: "Absence of α6(IV) collagen in kidney and skin of X-linked Alport syndrome." Pediatric Nephrology. 10. 742-744 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ljubinov,A V: "Basement membrane abnormalities in human eye with diabetic retinopathy." J.Histochem.Cytochem.44. 1469-1479 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Megumi Kagawa: "Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport's syndrome." Nephrology Dialysis Transplantation. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 二宮善文: "細胞外マトリックス-臨床医学への応用-" メヂカルレビュー社, 519 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Glew R.H.and Ninomiya Y.: "Clinical Studies in Medical Biochemistry" Oxford University Press, 449 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sugihara K.: "Experimental Anti-GBM glomerular nephritis induced in rats by immunization with syhthetic peptides based on six a chains of human type IV collagen." J.Pathol.178. 352-358 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ljubimov A.V.: "Extracellular alterations in human corneas with bullous keratopathy." Invest.Ophthal mol.Vis.Sci.37. 997-1007 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Rauf S.: "Nephritogenicity and a-chain composition of NC1 fractions of type IV collagen from bovine renal basement membrane." Virchow Archiv. 428. 281-288 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hino S: "Absence of alpha6 (IV) collagen in kidney and skin of X-linked Alport syndrome." Pediatric Nephrology. 10. 742-744 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ljubinov A.V: "Basement membrane abnormalities in human eye with diabetic retinopathy." J.Histochem.Cytochem.44. 1469-1479 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Megumi Kagawa: "Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport's syndrome." Nephrology Dialysis Transplantation. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshifumi Ninomiya: Medical Review Inc.Extracellular Matrix : Application to Clinical Medicine, 1-519 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Glew R.H.and Ninomiya Y.: Oxford University Press. Clinical Studies in Medical Biochemistry, 1-449 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sugihara K.: "Experimental Anti-GBM glomerular nephritis induced in rats by immunization with syhthetic peptides based on six a chains of human type IV collagen." J.Pathol. 178. 352-358 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ljubimov A.V.: "Extracellular alterations in human corneas with bullous keratopathy." Invest.Ophthal mol.Vis.Sci.37. 997-1007 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Rauf S.: "Nephritogenicity and a-chain composition of NCl fractions of type IV collagen from bovine renal basement membrane." Virchow Archiv. 428. 281-288 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hino S: "Absence of α6(IV) collagen in kidney and skin of X-linked Alport syndrome." Pediatric Nephrology. 10. 742-744 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ljubinov,AV: "Basemenet membrane abnormalities in human eye with diabetic retinopathy." J.Histochem.Cytochem.44. 1469-1479 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Megumi Kagawa: "Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport's syndrome." Nephrology Dialysis Transplantation. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 二宮善文: "細胞外マトリックス-臨床医学への応用" メヂカルレビュー社, 519 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Glew R.H.and Ninomiya Y.: "Clinical Studies in Medical Biochemistry" Oxford University Press, 449 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sado Y.: "Establishment of epitope-defined monoclonal antibodies recognizing the six different a chains of human type IV collagen by the rat lymph node method." Histochemistry and Cell Biology. 104. 267-275 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ninomiya Y.: "Differential expression of two basement membrane collagen genes,COL4A6 and COL4A5,demonstrated by immunofluorescence staining using peptide-specific monoclonal antibodies." J.Cell Biol.130. 1219-1229 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Oohashi T.: "Isolation and structure of genomic DNA encoding the human α6 (IV) collagen chain and comparison with other type IV collagen genes." J.Biol.Chem.270. 26863-26867 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sugihara K.: "Experimental Anti-GBM glomerular nephritis induced in rats by immunization with syhthetic peptides based on six a chains of human type IV collagen." J.Pathol.178(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ljubimov A.V.: "Extracellular alterations in human corneas with bullous keratopathy." Invest.Ophthal mol.Vis.Sci.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 二宮善文: "新しい基底膜コラーゲンα(IV)鎖とその遺伝子発現" 生化学, 1301-1307 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Karl Tryggvason and Yoshifumi Ninomiya: "Alport Syndrome (Hereditary Nephritis) In Clinical Studies in Medical Biochemistry,Eds.by Glew R.H.and Ninomiya Y." Oxford University Press,New York (in press), (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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