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Origin and Evolution of MHC

Research Project

Project/Area Number 07044288
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionNagoya City University

Principal Investigator

NONAKA Masaru  Nagoya City University, Medical School, Associate Professor, 医学部, 助教授 (40115259)

Co-Investigator(Kenkyū-buntansha) OZAKI Yasuhiko  Nagoya City University, Medical School, Research Associate, 医学部, 助手 (50254280)
CAMPBELL R.Duncan  University of Oxford, MRC,Special Appointment Grade, MRC, Special Ap
FLAJNIK Martin  University of Miami, School of Medicine, Professor, 医学部, 教授
CAMPBELL R.  オックスフォード大学, MRC, Special Ap
FLAJNIK Mar  マイアミ大学, 医学部, 教授
CAMPBELL Duc  オックスフォード大学, MRC, Special Ap
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1996: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1995: ¥3,600,000 (Direct Cost: ¥3,600,000)
KeywordsMHC / complement / lower vertebrate / evolution / 連鎖 / プロテアソーム
Research Abstract

The major histocompatibility complex (MHC), best described in mammals, is a large genetic region encompassing more than 4000kb in human. The Xenopus MHC is known to be remarkably similar to its mammalian counterparts. Class Ia, class IIa, class IIb, complement Bf, C4, heat shock protein 70 (HSP70), the proteasome subunit LMP7 genes all map to the Xenopus MHC,demonstrating that the overall organization and the basic genetic components were present at the time of the divergence of amphibians and mammals 300-350 million years ago. Furthermore, I detected a recombinant in previous family studies that demonstrated that the Xenopus LMP7/class II genes could be separated from the Bf/HSP70/class I suggesting that even the gene order is similar to that of mammals.
I found a second recombinant in another family where class II/LMP7/C4/class I genes cosegregate away from the Bf/HSP70 loci. Barring a double crossover, this recombinant implies that the class Ia gene is physically associated with the Xenopus class II region. This result is gratifying since the class I processing genes are found in the class II region in all species so far examined, and because class I and class II genes clearly have been derived from a common ancestor. An association with class II/III regions also may explain the remarkably deep and stable MHC-linked class I lineage in Xenopus ; I predict that the translocation of class I to the distal end of the MHC later in vertebrate evolution bestowed on class I genes the capacity to duplicate and diverge, hence giving rise to the celebrated class I plasticity found in mammals.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Mo,R.et al.: "Fourth component of Xenopus laevis complement:cDNA cloning and linkage analysis with frog MHC." Immunogenetics. 43. 360-369 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hatanaka,M.et al.: "Mechanism by which the surface expression of the glycosylphosphatidyl-inositol-anchored complement regylatory proteins decay-accelerating factor(CD55)and CD59 is lost in human leukemia cell lines" Biochem.J.314. 969-976 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kandil,E.et al.: "Isolation of low molecular mass polypeptide cDNA clones from primitive vertebrates:Implication for the origin of MHC class I restricted antigen presentation" J.Immunol.156. 4245-4253 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hamajima,N.et al.: "A novel gene family defined by human dihydropyrimidinase and three related proteins withdifferential tissue distribution" Gene. 180. 157-163 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kuroda,N.et al.: "Molecular cloning and linkage analysis of the Japanese medaka fish complement Bf/C2 gene." Immunogenetics. 44. 459-467 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hosokawa,M.et al.: "Molecular cloning of guinea pig membrane cofactorprotein(MCP):Preferential expression in testis." J.Immunol.157. 4946-4952 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mo, R.et al.: "Fourth component of Xenopus laevis complement : cDNA cloning and linkage analysis with frog MHC." Immunogenetics. 43. 360-369 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hatanaka, M.et al.: "Mechanism by which the surface expression of the glycosylphospha tidyl-inositol-anchored complement regylatory proteins decay-accele rating factor (CD55) and CD59 is lost in human leukemia cell lines." Biochem.J.314. 969-976 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kandil, E.et al.: "Isolation of low molecular mass polypeptide cDNA clones from primitive vertebrates : Implication for the origin of MHC class I-restricted antigen presentation." J.Immunol.156. 4245-4253 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hamajima, N.et al.: "A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution." Gene. 180. 157-163 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kuroda, N.et al.: "Molecular cloning and linkage analysis of the Japanese medaka fish complement Bf/C2 gene." Immunogenetics. 44. 459-467 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hosokawa, M.et al.: "Molecular cloning of guinea pig membrane cofactor protein (MCP) : Preferential expression in testis." J.Immunol.157. 4946-4952 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mo, R. et al.: "Fourth component of Xenopus laevis complement: cDNA cloning and linkage analysis with frog MHC." Immunogenetics. 43. 360-369 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hatanaka, M. et al.: "Mechanism by which the surface expression of the glycosylphosphatidyl-inositol-anchored complement regylatory proteins decay-accelerating factor(CD55)and CD59 is lost in human leukemia cell lines." Biochem. J.314. 969-976 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kandil, E. et al.: "Isolation of low molecular mass polypeptide cDNA clones from primitive vertebrates: Implication for the origin of MHC class I-restricted antigen presentation." J. Immunol.156. 4245-4253 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hamajima, N. et al.: "A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution." Gene. 180. 157-163 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kuroda, N. et al.: "Molecular cloning and linkage analysis of the Japanese medaka fish complement BF/C2 gene." Immunogenetics. 44. 459-467 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hosokawa, M. et al.: "Molecular cloning of guinea pig membrane cofactor protein(MCP): Preferential expression in testis." J. Immunol.157. 4946-4952 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kato,Y.,Salter-Cid,L.,Flajnik,M.F.,Namikawa,C.,Sasaki,M.,& Nonaka,M.: "Duplication of the MHC-linkeel Xeropus complement jactor B gene." Immunogenetics. 42. 196-203 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Namikawa,C.,Salter-Cid,L.,Flajnik,M.F.,Kato,Y.,Nonaka,M.& Sasaki,M.: "Isolation of Xenopus LMP 7 honologues : Striking allelic diversity and linkage to the MHC." J.Immunol.155. 1964-1971 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nonaka,M.,Miwa,T.,Okada,N.,Nonaka,M.& Okada,H.: "Multiple isoforms of guinea prg decay accelereting factor (DAF) generated by alternative splicing." J.Immunol. 155. 3037-3048 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] MO,R.,Kato,Y.,Nonaka,M.,Nakayama,K.& Takahashi,M.: "Fouth compoenet of Xenopus Laevis conplement:cDNA cloning and linkage analysty with frog MHC." Immunogenetics. im press. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ozaki,Y.,Aoki.K,Aoyama,T.,& Kuntmatsiu.M.: "Clinical Eignificancl of anti-GM3 antibodies in reciurent pregnoney loss with elevated level of antiphosphelipid antibodies." Am.J.Reprod.Immunol.33. 234-242 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Aguado,B.& Campbell,R.D.: "The nouel gene G17,loeated in the human major hisdocompatibility complix,encodes PBX2,ahomeodomaia-cohtainig protein." Genomics. 2535. 650-659 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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