Biochemical Collaboration on Bilogical Resources in South America

• Project/Area Number: 07045038
• Research Category: Grant-in-Aid for international Scientific Research
• Allocation Type: Single-year Grants
• Section: University-to-University Cooperative Research
• Research Field: Plant nutrition/Soil science
• Research Institution: Nagoya University
• Principal Investigator: SUGIYAMA Tatsuo Nagoya Univ., Sch.Agriculture, Prof., 農学部, 教授 (50023453)
• Co-Investigator(Kenkyū-buntansha): ANDREO C. ロザリオ大学, 生化学薬学部, 教授 ; GUIBERT Edgardo E. Rosario Univ., Sch.Biochemistry & Pharmacy, Assoc.Prof., 生化学薬学部, 助教授 ; TANIGUCHI Mitsutaka Nagoya Univ., Sch.Agriculture, Assoc.Prof., 農学部, 講師 (40231419) ; OGAWA Teruo Nagoya Univ., Bioscience Center, Prof., 生物分子応答研究センター, 教授 (80087593) ; TSUKAGOSHI Norihiro Nagoya Univ., Sch.Agriculture, Prof., 農学部, 教授 (50115599) ; ANDREO Carlos S. Rosario Univ., Sch.Biochemistry & Pharmacy, Prof. ; CABADA M.O. ロザリオ大学, 生化学薬学部, 教授 ; MENDOZA D.D. ロザリオ大学, 生化学薬学部, 教授 ; CARRILLO N.J ロザリオ大学, 生化学薬学部, 准教授 ; VIALE A.M. ロザリオ大学, 生化学薬学部, 准教授 ; 柳沼 利信 名古屋大学, 農学部, 講師 (60135332) ; 前多 敬一郎 名古屋大学, 農学部, 助教授 (30181580) ; 平嶋 義彦 名古屋大学, 農学部, 教授 (30208821) ; 武岡 洋治 名古屋大学, 農学部, 教授 (70023455) ; 末田 達彦 名古屋大学, 農学部, 助教授 (90109314) ; 大島 光昭 名古屋大学, 農学部, 教授 (20035976) ; CABADA M. O ロザリオ大学, 生化学薬学部, 教授 ; VIALE A. M ロザリオ大学, 生化学薬学部, 準教授 ; ANDREO C. S ロザリオ大学, 生化学薬学部, 教授 ; 山木 昭平 名古屋大学, 農学部, 教授 (70210341)
• Project Period (FY): 1995 – 1997
• Project Status: Completed (Fiscal Year 1997)
• Budget Amount: ¥6,600,000 (Direct Cost: ¥6,600,000); Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000); Fiscal Year 1996: ¥2,200,000 (Direct Cost: ¥2,200,000); Fiscal Year 1995: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: Nagoya University / Rosario University / Biochemical collaboration / South America / C4 plant / 南米食用植物 / 枯草菌 / シャペロン / 生化学的研究 / 南米固有 / 資源生物

Research Abstract

In the 3rd year of our research project, we attempted to develop the future joint researches on native plants and animals of South America. Ogawa and Taniguchi from Nagoya University and Guibert from Rosario University visited to each partner and exchanged their informations each other.
Ogawa and Taniguchi discussed about current researches on C_4 plants, which were originated from South America and were useful for grass or food, with plant researchers of Rosario University. They found researchers at Rosario University have been studying extensively to elucidate regulatory mechanisms of C_4 photosynthetic-proteins synthesis at both their activity and gene expression levels. They have concluded that some collaborative researches in these areas at molecular biological levels will be planned between the two institutions in future. Moreover, Ogawa and Taniguchi introduced current researches at Nagaya University to researchers of Rosario University and discussed what a future collaborative research should be.
Guibert had a seminar on freeze-storing of animal cells at Nagoya University. He also exchanged the in formation on current researches with mainly animal researchers of Nagoya University and discussed avidly future collaborative researches.

Research Products

• [Publications] 杉山 達夫: "C4植物における光合成機能統御の分子機構の研究" 日本農芸化学会誌. 72. 145-151 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sakakibara, H.: "Partial characterization of signaling pathway of nitrate-dependent expression of the genes for nitrogen-assimilatory enzymes using detached maize leaves" Plant Cell Physiol.38. 837-843 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kato, M.: "An Aspergillus nidulans nuclear protein,AnCP,involved in enhancement of Taka-amylas A gene expression,binds to the CCAAT-containing taaG2,amdS and gatA promoters." Mol.Gen.Genet.254. 119-126 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sonoda, M.: "Size of cotA and identification of the gene product in Synechocystissp.strain PCC6803" J.Bacteriol.179. 3845-3850 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Taniguchi, M.: "The expression of 2-oxoglutarate/malate translocator in the bundle-sheath mitochondria of Panicummiliaceum,a NAD-malic enzyme-type C4 plant,is regulated by light and development." Plant Physiol.114. 285-293 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Maurino, V.G.: "NADP-malic enzyme:immunolocalization in different tissues of the C4 plant maize and the C3 plant wheat" J.Exp.Bot.48. 799-811 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sugiyam, T.: "Stress Responses of Photosynthetic Organisms" Elsevier, 4 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iglesias, A.A.: "Handbook of Photosynthesis" Marcel Dekker, 13 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sugiyama, T.: "Molecular mechanism for regulation of organization of photosynthesis in C_4 plants." Nippon Nodgeikagaku Kaishi. 72. 145-151 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sakakibara, H., Kobayashi, K., Deji, A.and Sugiyama, T.: "Partial characterization of signalling pathway of nitrate-dependent expression of the genes for nitrogen-assimilatory enzymes using detached maize leaves." Plant Cell Physiol.38. 837-843 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kato, M., Aoyama, A., Naruse, F., Kobayashi, T.and Tsukagoshi, N.: "An Aspergillus nidulans nuclear protein, An CP,in volved in enhancement of Taka-amylase A gene expression, binds to the CCAAT-containing taaG2, amdS and gatA promoters." Mol.Gen.Genet.254. 119-126 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sonoda, M., Kitano, K., Katoh, A,.Katoh.H., Ohkawa, H.and Ogawa, T.: "Size of cotA and diden tification of the gene productin Synechocystis sp. strain PCC6803." J.Bacteriol.179. 3845-3850 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Taniguchi, M.and Sugiyama, T.: "The expression of 2oxoglutarate/malate translocator in the bundle-sheath mitochondria of Panicum miliaceum, a NAD-malic enzyme-type C_4 plant, is regulated by light and development." Plant Physiol.114. 285-293 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Maurino, V.G., Drincovich, M.F., Casati, P., Andreo, C.S., Edwards, G.E., Ku, M.S.B., Gupta, S.K,and Franceschi, V.R.: "NADP-malic enzyme : immunolocalization in different tissues of the C_4 plant maize and the C_3 plant wheat." J.Exp.Bot.48. 799-811 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] 杉山 達夫: "C4植物における光合成機能統御の分子機構の研究" 日本農芸化学会誌. 72. 145-151 (1998)
• [Publications] Sakakibara, H.: "Partial characterization of signalling pathway of nitrate-dependent expression of the genes for nitrogen-assimilatory enzymes using detached maize leaves" Plant Cell Physiol.38. 837-843 (1997)
• [Publications] Kato, M.: "An Aspergillus nidulans nuclear protein,AnCP,involved in enhancement of Taka-amylase A gene expression,binds to the CCAAT-containing taaG2,amdS and gatA promoters." Mol.Gen.Genet.254. 119-126 (1997)
• [Publications] Sonoda, M.: "Size of cotA and identification of the gene product in Synechocystis sp.strain PCC6803" J.Bacteriol.179. 3845-3850 (1997)
• [Publications] Taniguchi, M.: "The expression of 2-oxoglutarate/malate translocator in the bundlesheath mitochondria of Panicum miliaceum,a NAD-malic enzymetype C4 plant,is regulated by light and development." Plant Physiol.114. 285-293 (1997)
• [Publications] Maurino, V.G.: "NADP-malic enzyme:immunolocalization in different tissues of the C4 plant maize and the C3 plant wheat" J.Exp.Bot.48. 799-811 (1997)
• [Publications] Sugiyama, T.: "Stress Responses of Photosynthetic Organisms" Elsevier, 4 (1998)
• [Publications] Iglesias, A.A.: "Handbook of Photosynthesis" Marcel Dekker, 13 (1997)
• [Publications] Kikuchi,H.et al.: "Positive regulation by nitrite of the transcription of nitrate assimilation operon in the cyanobacteria Synechococcus sp.strain PCC7942 and Plectonema boryanum." J.Bacteriol.178. 5822-5825 (1996)
• [Publications] Sakakibara,H.et al.: "Molecular identification and characterization of cytosolic isoforms of glutamine synthetase in maize roots." J.Biol.Chem.271. 29561-29568 (1996)
• [Publications] Sakakibara,H.et al.: "Isolation and characterization of a cDNA that encodes maize uroporphyrinogen III methyl transferase,an enzyme involved in the synthesis of siroheme,which is prosthetic group of nitrite reducatase" Plant J.10. 883-892 (1996)
• [Publications] Suzuki,I.et al.: "Regulation by cyanate of the genes involved in carbon and nitrogen assimilation in the cyanobacterium Synechococcus sp.strain PCC 7942." J.Bacteriol.178. 2688-2694 (1996)
• [Publications] Taniguchi,M.et al.: "Isolation,characterization and expression of cDNA clones encoding a mitochondrial malate translocator from panicum miliceum L." Plant Mol.Biol.30. 51-64 (1996)
• [Publications] Torii,Y.et al.: "Synergistic effect of BMP-2 and ascorbate on the phenotypic expression of osteoblastic MC3T3-E1 cells." Mol.Cell.Biochem.165. 25-29 (1996)
• [Publications] Torii,Y.et al.: "Demonstration of alkaline phosphatase participation in the mineralization of osteoblasts by antisense RNA approach." Cell Bol.Int.20. 459-464 (1996)
• [Publications] Kitamoto,N.et al.: "Molecular cloning,purification and characterization of two endo-1,4-glucanases from Aspergillus oryzae KBN616." App.Microbiol.Biotechnol.46. 538-544 (1996)
• [Publications] Kato,M.et al.: "Sequence-specific binding sites in the Taka-amylase A G2 promoter for the CreA repressor mediating carbon catabolite repression." Biosci.Biotechnol.Biochem.60. 1776-1779 (1996)
• [Publications] Suzuki, I. et al.: "Identification and characterization of two nitrogen-regulated genes of the cyanobacterium Synechococcus sp. strain PCC7942 required for maximum efficiency of nitrogen assimilation." J. Bacteriol.,. 177. 290-296 (1995)
• [Publications] Suzuki, I. et al.: "Regulation of nitrite reductase activity under CO2 limitation in the cyanobacterium Synechococcus sp. PCC7942." Plant Physiol.,. 107. 791-796 (1995)
• [Publications] Taniguchi, M. et al.: "Aspartate aminotransferase isozymes in Panicum miliaceum L., an NAD-malic enzyme-type C_4 plant:comparison of enzymatic properties, primary stractures, and expression patterns." Arch. Biochem. Biophys.318. 295-306 (1995)
• [Publications] Ishihara, T. et al.: "Cloning and characterization of the gene for a protein thiol-disulfide oxidoreductase in Bacillus brevis." J. Bacteriol.,. 177. 745-749 (1995)
• [Publications] Kitamoto, N. et al.: "The nitrate reductase from a Shoyu koji mold, Aspergillus oryzae KBN616." Biosci. Biotech. Biochem.,. 59. 1795-1797 (1995)
• [Publications] Yoshino, S. et al.: "Two family G xylanase genes from Chaetomium gracile and their expression in Aspergillus nidulans." Curr. Genet.,. 29. 73-80 (1995)
• [Publications] Kanayama, Y. et al.: "Expression of the gene for NADP-dependent sorbitol-6-phosphate dehydrogenase from apple fruit." Plant Cell Physiol.,. 36. 1139-1141 (1995)
• [Publications] Yamaki, S.: "Physiology and metabolism of fruit development-Biochemistry of sugar metabolism and compartmentation in fruits." Acta Hort.,. 398. 109-120 (1995)
• [Publications] Meda, K. -I. et al.: "Novel feedback sites associated with stress-induced suppression of luteinnizing hormone section in female rats." Cell. Mol. Neurobiol.,. (in press). (1996)
• [Publications] Murahashi, K. et al.: "Suppression of LH pulses by restriction of glucose availability is mediated by sensors in the brain stem." Endocrinol.,. (in press). (1996)
• [Publications] Nagatani, S. et al.: "Reduction of glucose availability suppresses pulsatile LH release in female and male rats." Endocrinol.,. (in press). (1996)
• [Publications] Yaginuma, T. et al.: "Trchalase in the spermatophore from the bean-shaped accessory gland of the male mealworm beetle, Tenebrio molitor: purification, kinetic properties and localization of the enzyme." J. Comp. Physiol. B.,. 166(in press). (1996)