| Project/Area Number |
07101001
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| Research Category |
Grant-in-Aid for Specially Promoted Research
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SAKANO Hitoshi Graduate School of Science, The University of Tokyo, Professor, 大学院・理学系研究科, 教授 (90262154)
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| Co-Investigator(Kenkyū-buntansha) |
TSUBOI Akio Graduate School of Science, The University of Tokyo, Research Associate, 大学院・理学系研究科, 助手 (20163868)
NAGAWA Fumikiyo Graduate School of Science, The University of Tokyo, Lecturer, 大学院・理学系研究科, 講師 (10241233)
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| Project Period (FY) |
1995 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥249,000,000 (Direct Cost: ¥249,000,000)
Fiscal Year 1999: ¥43,000,000 (Direct Cost: ¥43,000,000)
Fiscal Year 1998: ¥46,000,000 (Direct Cost: ¥46,000,000)
Fiscal Year 1997: ¥46,000,000 (Direct Cost: ¥46,000,000)
Fiscal Year 1996: ¥55,000,000 (Direct Cost: ¥55,000,000)
Fiscal Year 1995: ¥59,000,000 (Direct Cost: ¥59,000,000)
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| Keywords | V(D)J joining / RSS signals / RAG proteins / RAG / RSS complex / footprinting / 3'-phosphorylation / transposition / 12 / 23 rule / 抗原受容体遺伝子 / 免疫グロブリン遺伝子 / 組み換えシグナル配列 / リコンビナーゼ / リンパ細胞の分化 / 組み換え制御因子 / トランスジェニックマウス / 臭覚受容体遺伝子 / V(D)J結合 / エンハンサー / 対立形質排除 / 体細胞突然変異 / エンハンサーエレメント / 組み換え抑制因子 |
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| Research Abstract |
We have studied the interaction between recombination signal sequences (RSSs) and protein products of the recombination-activating genes (RAGs). Comparison of the RAG-RSS footprint data with the published Hin model confirmed the notion that sequence-specific RSS-RAG interaction takes place primarily between the Hin homeodomain of the RAG1 protein and adjacent major and minor grooves of the 9mer DNA.
When two RSSs are cleaved in V(D)J joining, the signal-end (SE) complex is formed with RAG1 and RAG2 proteins in the presence of DNA bending protein HMG1. Using magnetic beads, we have purified the SE complex and analyzed the SE DNA by DNaseI footprinting. It was found that the 7mer rather than the 9mer plays a primary role in the SE complex formation. It was also found that the 3'-OH of the 7mer is phosphorylated in the SE complex in both 12- and 23-RSS's after the cleavage. The 3'-phosphate is transferred from carrier DNA or RNA by trans-esterification after nicking of the carrier. In vitro assays demonstrated that the 3'-phosphorylation prevents the transposition of the SE complex. These results indicate that the 3'-phosphorylation may have an active role in preventing harmful transposition of an excision product of V(D)J joining.
We have shown that the PU.1 binding motif (GAGGAA) in the 3'-enhancer of the IgィイD2κィエD2 gene is responsible for the negative regulation of VィイD2κィエD2-JィイD2κィエD2 joining. In the transcriptional activation, the PU.1 motif acts in a positive manner cooperatively with the PIP and BSAP motifs, which are located in the same enhancer core region. Interestingly, base substitutions in either the PIP or the BSAP motif did not affect the B/T or pro-B/pre-B specificity of VィイD2κィエD2-JィイD2κィエD2 joining. Thus, the PU.1 motif alone appears to regulate both temporal and tissue-specific rearrangements of the IgィイD2κィエD2 gene.
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