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Study on the regulatory mechanisms of endothelium-dependent vasoconstriction and growth of vascular cells

Research Project

Project/Area Number 07101002
Research Category

Grant-in-Aid for Specially Promoted Research

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Cardiovascular Center Research Institute (1997-1998)
Kyoto University (1995-1996)

Principal Investigator

MASAKI Tomoh  National Cardiovascular Center Research Institute, Director General, 所長 (60009991)

Co-Investigator(Kenkyū-buntansha) SAWAMURA Tatsuya  National Cardiovascular Center Research Institute, Head, バイオサイエンス部, 室長 (30243033)
岡本 安雄  京都大学, 医学研究科, 助手
二宮 治明  京都大学, 医学研究科, 助手 (80212124)
三輪 聡一  京都大学, 医学研究科, 助教授 (40157706)
比企 一晃  京都大学, 医学研究科, 助手 (30263099)
Project Period (FY) 1995 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥283,000,000 (Direct Cost: ¥283,000,000)
Fiscal Year 1998: ¥23,000,000 (Direct Cost: ¥23,000,000)
Fiscal Year 1997: ¥63,000,000 (Direct Cost: ¥63,000,000)
Fiscal Year 1996: ¥82,000,000 (Direct Cost: ¥82,000,000)
Fiscal Year 1995: ¥115,000,000 (Direct Cost: ¥115,000,000)
Keywordsvascular endothelium / endothelin / nitric oxide / oxidized LDL / endothelin receptor / vasoconstriction / cell growth, dystroglycan / ジストログリカン / 変性LDL受容体 / アルギニン輸送体 / 非選択的カチオンチャネル / 受容体輸送活性相関 / 変成LDL受容体 / 電位依存性カルシウムチャネル / 非選択的陽イオンチャネル / エンドセリン変換酵素 / カチオンチャネル
Research Abstract

To elucidate regulatory role for endothelium in local vascular function. we have studied cellular and subcellular mechanisms of endothelin(ET) action and interaction between ET and nitric oxide(NO). A novel endothelial receptor for oxidized LDL was cloned. It phagocytized the aged blood cells and the apoptized cells as well as oxidized LDL.It also suppressed NO synthesis via inhibition of cationic amino acid transporter, and increased ET synthesis. ET induced smooth muscle contraction by activation of non-selective cation channel sensitive to SK&F96365. This channel was also inhibited by NO.Both ET_A and ET_B coupled to Galphas, Galphai, and Galphaq. However, each of these Galpha proteins bound to different part of the domain of ET receptor. Palmitoylation of C-terminal cystein residues of ET receptor was particularly important to the binding of Galphai and Galphaq. Cell growth activity of ET was mediated by three ways, i.e. activation of protein kinase C, phosphoinositide 3-kinase, and thyrosin kinase pathways. MAP kinase was activated in every pathway. Dystroglycan was shown to be expressed in endothelium. It was shown that dystroglycan regulated motility of endothelial cells.

Report

(5 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (72 results)

All Other

All Publications (72 results)

  • [Publications] Y.Takagi et al: "structural basis of G protein specificity of human endothelin receptors : a study with endothelin _<A/n> chimeras." J.Biol.Chem.270. 10072-10078 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Sawamura et al: "An endothelial receptor for oxidized low-density lipoproteins." Nature. 286. 73-77 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Hosokawa et al: "Cloning and characterization of a brain-specific cationic amino acid transporter." J.Biol.Chem.272. 8717-8722 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Oka et al.: "Lectin-like oxidized low-density lipoprotein receptor I mediates phagocytosis of aged/apoptotic cells in endothelial cells" Proc.Natl.Acad Sci.USA. 95. 9535-9540 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Kikuta et al: "High-affinity arginine transport of bovine aortic endothelial cells impaired by lysophosphatidylcholine" Circ.Res.83. 1088-1096 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Aoyama et al: "Structure and chromosomal assignment of human lectin-like oxidized LDL receptor-1(LOX-1)" Biochem.J.339. 177-184 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Enoki, et al.: "Long-lasting activation of cation current by low concentration of endothelin-1 in mouse fibroblasts and smooth muscle cells of rabbit aorta." Br.J.Pharmacol.115. 479-485 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Takagi et al.: "Structural basis of G protein specificity of human endothelin receptors : a study with endothelin A/B chimeras." J.Biol.Chem.270. 10072-10078 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Fujitani et al.: "Suppression of endothelin-1-induced mitogenic responses of human aortic smooth muscle cells by interleukin-1beta" J.Clin.Invest.95. 2474-2482 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Okamoto et al.: "Capacitative Ca2+ entry in human platelets is resistant to nitric oxide." Biochem.Biophys.Res.Commun.212. 90-96 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Ninomiya et al.: "Distinct roles of second and third intracellular loops of human endothelin receptors in the selective activation of Galphas/Galphai." J.Cardiovasc.Pharmacol.26(Supp.3). S254-S257 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Enoki et al.: "Functional coupling of ETA receptor with Ca2+-permeable nonselective cation channel in mouse fibroblasts and rabbit aortic smooth-muscle cells." J.Cardioyasc.Pharmacol.26(Supp.3). S258-S261 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Koshimizu et al.: "Truncation of the receptor carboxy1 terminus impairs membrane signaling but not ligand binding of human ETB" Biochem.Biophys.Res.Commun. 217. 354-362 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] F.Sugawara et al.: "Endothelin-1-induced mitogenic responses of Chinese hamster ovary cells expressing human endothelinA : the role of wortmannin-sensitive signaling pathway." Mol.Pharmacol.49. 447-457 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Shiraki et al.: "Stimulating effect of 6R-tetrahydrobiopterin on Ca2+ channels in neurons of rat dorsal motor nucleus of the vagus." Biochem.Biophys.Res.Commun.221. 181-185 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] R.Matsuoka et al.: "Human endothelin converting enzyme gene mapped to chromosomal region 1p36.1." Cytogenet.Cell Genet.72. 322-324 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Komuro et al.: "The involvement of a novel mechanism distinct from the thrombin receptor in the vasocontraction induced by trypsin." Br.J.Pharmacol. 120. 851-856 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Sawamura, et al.: "An endothelial receptor for oxidized low-density lipoproteins." Nature. 386. 73-77 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Hosokawa, et al.: "Cloning and characterization of a brain-specific cationic amino acid transporter." J.Biol.Chem.272. 8717-8722 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Kido, et al.: "Processing of proendothelin-1 at the C-terminus of big endothelin-1 is essential for proteolysis by endothelin-converting enzyme-1 in vivo." Eur.J.Biochem.244. 520-526 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Minowa et al.: "Inhibitory effect of nitrovasodilators and cyclic GMP on ET-1-activated Ca2+-permeable nonselective cation channel in rat aortic smooth muscle cells." Br.J.Pharmaol.120. 1536-1544 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ohtani et al.: "Bromodeoxyuridine-induced expression of endothelinA in A375 human melanoma cells." Biochem.Biophys.Res.Commun.234. 526-530 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Okamoto et al.: "Palmitoylation of human endothelinB." J.Biol.Chem. 272. 21589-21596 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Komuro et al.: "Physiological role of Ca2+-permeable nonselective cation channel in endothelin-1-induced contraction of rabbit aorta." J.Cardiovasc.Pharmacol.30. 504-509 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Kido et al.: "The processing pathway of endothelin-1 production." J.Cardiovas.Pharmacol.31(Suppl.1). S13-S15 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Okamoto et al.: "Cysteine residues in the carboxyl terminal domain of the endothelin-B receptor are required for coupling with G-proteins" J.Cardiovas.Pharmacol.31(Suppl.1). S230-S232 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Okazawa et al.: "Endothelin-induced apoptosis of A375 human melanoma cells" J.Biol.Chem.273. 12584-12592 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Hoshikawa et al.: "High affinity binding of oxidized LDL to mouse lectin-like oxidized LDL receptor (LOX-1)" Biochem.Biophys.Res.Commun.254. 841-846 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Ninomiya et al.: "Paracrine endothelin signaling in the control of basal cell proliferation in guinea pig tracheal epithelium" J.Pharmacol.Exp.Ther.286. 469-480 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Hasegawa et al.: "Purification of a novel endothelin-converting enzyme specific for big endothelin-3" FEBS Letters. 428. 304-308 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Oka et al.: "Lectin-like oxidized low-density lipoprotein receptor 1 mediates phagocytosis of aged/apoptotic cells in endothelial cells" Proc.Natl.Acad Sci.USA. 95. 9535-9540 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Iwamuro et al.: "Activation of two types of Ca2+-permeable nonselective cation channel by endothelin-1 in A7r5 cells." Br.J.Pharmacol.124. 1541-1549 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] X.F.Zhang et al.: "Role of nonselective cation channels as Ca2+ entry pathway in endothelin-1-induced contraction and their suppression by nitric oxide" Eur.J.Pharmacol.352. 237-245 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Ono et al.: "Desensitization of ETA endothelin receptor-mediated negative chronotropic response in right atria-species difference and intracellular mechanisms" Br.J.Pharmacol.125. 787-797 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Kikuta et al.: "High-affinity arginine transport of bovine aortic endothelial cells is impaired by lysophosphatidy lcholine" Circ.Res.83. 1088-1096 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Hasegawa et al.: "Purification of a novel endothelin-converling enzyme specific for big endothelin-3" FEBS Letters. 428. 304-308 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Oka et al.: "Lectin-like oxidized low-density lipoprotein receptor I mediates phagocytosis of aged/apoptotic cells in endothelial cells" Proc.Natl.Acad Sci.USA. 95. 9535-9540 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Iwamuro et al.: "Activation of two types of Ca^<2+> -permeable nonselective cation channel by endothelin-1 in A7r5 cells" Br.J.Pharmacol.124. 1541-1549 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] N.Kume et al.: "Inducible expression of Lectin-like oxidized LDL receptor-I in vascular endothelial cells" Circ.Res.83. 322-327 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Murase et al.: "Fluid shear stress transcriptionally induces Lectin-like oxidezed LDL receptor-1 in vascular endothelial cells" Circ.Res.83. 328-333 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] X.F.Zhang et al.: "Role of nonselective cation channels as Ca^<2+> entry pathway in endothelin-1-induced contraction and their suppression by nitric oxide" Eur.J.Pharmacol.352. 237-245 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Ono et al.: "Desensitization of ET_A endothelin receptor-inediated negative chronotropic response in right atria-species difference and intracellular mechanisms" Br.J.Pharmacol.125. 787-797 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Kido et al.: "The processing pathway of endothelin-1 production." J.Cardiovas.Pharmacol.31,Suppl.1. S13-S15 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Okamoto et al.: "Cysteine residues in the carboxyl terminal domain of the endothelin-B receptor are required for coupling with G-proteins" J.Cardiovas.Pharmacol.31,Suppl.1. S230-S232 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Okazawa et al.: "Endothelin-induced apoptosis of A375 human melanoma cells" J.Biol.Chem.273. 12584-12592 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Tanaka et al.: "Novel mutations of the endothelin B receptor gene in patients with hirschsprung's disease and their characterization" J.Biol.Chem.273. 11378-11383 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] B.Nicholson et al.: "Increased Cat3-mediated cationic amino acid transport functionally compensates in cat1 knockout cell lines" J.Biol.Chem.273. 14663-14666 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Hoshikawa et al.: "Iligh affinity binding of oxidized LDL to mouse lectin-like oxidized LDL receptor(LOX-1)" Biochem.Biophys.Res.Commun.245. 841-846 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Ninomiya et al.: "Paracrine endothelin signaling in the control of basal cell proliferation in guinea pig tracheal epithelium" J.Pharmacol.Exp.Ther.286. 469-480 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Kikuta et al.: "High-affinity arginine transport of bovine aortic endothelial cells is impaired by lysophosphatidylcholine" Circ.Res.83. 1088-1096 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Iwamuro et al.: "Activation of three types of voltage-independent Ca^<2+> -entry channel in A7r5 cells by endothelin-1 as revealed by a novel Ca^<2+> -entry channel blocker LOE 908" Br.J.Pharmacol.(in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Aoyama et al.: "Structure and chromosomal assignment of human lectin-like oxidized LDL receptor-1(LOX-1)" Biochem.J.,in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Inuri et al.: "Selective activation of excitation-contraction coupling pathways by ET_A and ET_B in tracheal smooth muscle" Br.J.Pharmacol.,. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Sawamura,et al.: "An endothelial receptor for oxidized low-density lipoproteins." Nature. 386. 73-77 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Ohtani,et al.: "Bromodeoxyuridine-induced expression of endothelin in A375 human melanoma cells." Biochem.Biophys.Res.Commun.234. 526-530 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Okamoto,et al.: "Palmitoylation of human endothelin_B." J.Biol.Chem.272. 21589-21596 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Komuro,et al.: "Physiological role of Ca^<2+>-permeable nonselective cation channel in endothelin-induced contraction of rabbit aorta." J.Cardiovasc.Pharmacol.30. 504-509 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Sakamoto,et al.: "Both hypertrophic and dilated cardiomyopathies are caused by mutation of the same gene,α-sarcoglycan.in hamster:an animal model of disrupted dystrophin-associated glycoprotein" Proc.Natl.Acad.Sci.USA. 94. 13873-13878 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Okazawa,et al.: "Endothelin-induced apoptosis of A375 human melanoma cells" J.Biol.Chem.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiroshi Hosokawa et al.: "Cloning and characterization of a brain-specific cationic amino acid transporter" J.Biol.Chem.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsuneo Kido et al.: "Processing of proendothelin-1 at the C-terminus of big endothelin-1 is essential for proteolysis by endothelin-converting enzyme-1 in vivo" Eur.J.Biochem.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Tatsuya Sawamura et al.: "An endothelial receptor for oxidized low-density lipoproteins" Nature. 386. 73-77 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Taro Komuro et al.: "A physiological role of Ca^<2+>-permeable nonselective cation channel in endothelin-1-induced contraction of rabbit aorta" J.Cardiovascul.Pharmacol.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Taro Komuro et al.: "The involvement of a novel mechanism distinct from the thrombin receptor in the vasocontraction induced by trypsin" Brif J.Pharmacol.120. 851-856 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tetsuya Minowa et al.: "Inhibitory effect of nitrovasodilators and cyclic GMP on ET-1-activated Ca^<2+>-permeable nonselective cation channel in rat aortic smooth muscle cells" Brif,J.Pharmacol.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.okamoto: "Capacitative Ca^<2+> entry in human platelets is resistant to nitric oxide." Biochem.Biophys.Res.Commun.212. 90-96

    • Related Report
      1995 Annual Research Report
  • [Publications] J.B.Denault: "Inhibition of convertase-related processing of proendothelin-1." J.Cardiovasc.Pharmacol.26(Suppl.3). S47-S50

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Ninomiya: "Distinct roles of second and third intracellular loops of human endothelin receptors in the selective activation of Gαs/Gαi." J.Cardiovasc.Pharmacol.26(Suppl.3). S254-S257

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Enoki: "Functional coupling of ETA receptor with Ca^<2+> -permeable nonselective cation channel in mouse fibroblasts and rabbit aortic smooth-muscle cells." J.Cardiovasc.Pharmacol.26(Suppl.3). S258-S261

    • Related Report
      1995 Annual Research Report
  • [Publications] R.Matsuoka: "Human endothelin converting enzyme gene mapped to chromosomal region 1p36.1." Cytogenetics.Cell Genetics.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] F.Sugawara: "Endothelin-1-induced mitogenic responses of Chinese hamster ovary cells expressing human endothelin A:The role of Wortmininnin-sensitive signaling pathway." Mol.Pharmacol.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Masaki: "The discovery of endothelins:Impact on biomedicine.Endothelin:Role in Health and Disease" Harwood Academic Publishers gmbh., 11

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2018-03-28  

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