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Host factors involved in development of poliomyelitis

Research Project

Project/Area Number 07102005
Research Category

Grant-in-Aid for Specially Promoted Research

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo

Principal Investigator

NOMOTO Akio  Institute of Medical Science, The University of Tokyo, Professor, 医科学研究所, 教授 (70112670)

Project Period (FY) 1995 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥273,000,000 (Direct Cost: ¥273,000,000)
Fiscal Year 1999: ¥49,000,000 (Direct Cost: ¥49,000,000)
Fiscal Year 1998: ¥49,000,000 (Direct Cost: ¥49,000,000)
Fiscal Year 1997: ¥49,000,000 (Direct Cost: ¥49,000,000)
Fiscal Year 1996: ¥51,000,000 (Direct Cost: ¥51,000,000)
Fiscal Year 1995: ¥75,000,000 (Direct Cost: ¥75,000,000)
Keywordspoliovirus / mouse model / poliovirus receptor / pathogenesis / dissemination / IRES / axonal transport / host / 感染マウスモデル / 脳関門 / 宿主分子群 / 種特異性 / 複製 / 宿主分子 / トランスジェニックマウス / アデノウイルスベクター
Research Abstract

Poliovirus (PV) infection induced change in patterns of two-dimensional gel electrophoresis of total proteins of HeLa cells. Identification of proteins of these spots is now being investigated.
To support our idea of "IRES (internal ribosome entry site)-dependent virus tropism", a chimera PV, in which PV IRES is replaced by hepatitis C virus (HCV) IRES, was constructed. The chimera virus was examined for its replication ability in the liver and the central nervous system (CNS) of poliovirus-sensitive transgenic mice. Replication rate of the chimera virus was compared with that of the parental PV. The results have suggested that PV IRES works both in the liver and CNS, and that HCV IRES works in the liver but not in the CNS.
We demonstrated that interaction of the cytoplasmic domain (cyto) of human PV receptor (hPVR) with cytoplasmic dynein light chain 1 may have an important role in retrograde axonal transport of PV. To identify key amino acids of hPVR-cyto involved in the interaction, a … More series of deletion mutants of hPVR-cyto were constructed and tested for their binding activity to dynein molecule. The results suggested that N-terminal 20 amino acids of hPVR-cyto were essential for the interaction.
The hPVR has been thought to have a dual function, that is, binding ability to PV and conformation alteration ability of PV particle. To know how these two abilities contribute to establishment of PV infection, mouse cell line expressing Fcγ receptor was established and named LmFcRI cell line. As mediators of PV and LmFcRI cells, several anti-PV monoclonal antibodies and PVR-IgG2a which is a fusion protein of the extracellular domain of hPVR and Fc portion of mouse IgG2a was used. The results have suggested that binding of PV on the cell surface induces PV infection at very low level, and that specific interaction of hPVR with PV affords a high level of infectivity to PV. This enhancement of infectivity induced by hPVR may be essential for establishment of PV infection in a whole body. Less

Report

(6 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (50 results)

All Other

All Publications (50 results)

  • [Publications] Minetaro Arita: "Interaction of poliovirus with its receptor affords a high level of infectivity to the virion in poliovirus infections mediated by the Fc receptor"J.Virol.. 73(2). 1066-1074 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kazuko Shiroki: "Intracellular redistribution of truncated La protein produced by poliovirus 3C^<pro>-mediated cleavage"J.Virol.. 73(3). 2193-2200 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiko Ishii: "Identification of a new element for RNA replication within the internal ribosome entry site of poliovirus RNA"J.Gen.Virol.. 80(4). 917-920 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Minetaro Arita: "Multiple pathways for establishment of poliovirus infection"Virus Res.. 62. 97-105 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takeshi Isoyama: "Lower concentration of La protein required for internal ribosome entry on hepatitis C virus RNA than poliovirus RNA"J.Gen.Virol.. 80(9). 2319-2327 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Qingmei Jia: "Isolation and molecular characterization of a poliovirus type 1 mutant that replicates in the spinal cord of mice"J.Virol.. 73(7). 6041-6047 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shusuke Kuge: "Redox Regulation of Cell Signaling Its Clinical Application"Marcel Dekker,Inc.. 328 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arita, M., Horie, H., Arita, M., and Nomoto. A.: "Interaction of poliovirus with its receptor affords a high level of infectivity to the virion in poliovirus infections mediated by the Fc receptor"J. Virol.. 73(2). 1066-1074 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shiroki, K., Isoyama, T., Kuge, S., Ishii, T., Ohmi, S., Hata, S., Suzuki, K., Takasaki, Y., and Nomoto, A.: "Intracellular redistribution of truncated La protein produced by poliovirus 3CPRO-mediated cleavage"J. Virol.. 73(3). 2193-2200 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishii, T., Shiroki, K., Iwai, A., and Nomoto, A.: "Identification of a new element for RNA replication within the intenal ribosome entry site of poliovirus RNA"J. Gen. Virol.. 80(4). 917-920 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arita, M., Onka, S., and Nomoto, A.: "Multiple pathways for establishment of poliovirus infection"Virus Res.. 62. 97-105 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Isoyama, T., Kamoshita, N., Yasui, K., Iwai, A., Shiroki, K., Toyoda. H., Yamada, A., and Nomoto, A.: "Lower concentration of La protein required for internal ribosome entry on hepatitis C virus RNA than poliovirus RNA"J. Gen. Virol.. 80(9). 2319-2327 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Jia, Q., Onka, S., Iwasaki, K., and Nomoto, A.: "Isolation and molecular characterization of a poliovirus type 1 mutant that replicates in the spinal cord of mice"J. Virol.. 73(7). 6041-6047 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kuge, S., Nomoto, A., and Jones, N.: "Regulation of yAP-1 nuclear localization in response to oxidative stress"In Redox Regulation of Cell Signaling and Its Clinical Application, Lester Packer, Junji Yodoi, eds., Marcel Dekker, Inc., New York, Basel. 73-84 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Minetaro Arita: "Interaction of poliovirus with its receptor affords a high level of infectivity to the virion in poliovirus infections mediated by the Fc receptor"J. Virol.. 73(2). 1066-1074 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kazuko Shiroki: "Intracellular redistribution of truncated La protein produced by poliovirus 3C^<pro>-mediated cleavage"J. Virol.. 73(3). 2193-2200 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiko Ishii: "Identification of a new element for RNA replication within the internal ribosome entry site of poliovirus RNA"J. Gen. Virol.. 80(4). 917-920 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Minetaro Arita: "Multiple pathways for establishment of poliovirus infection"Virus Res.. 62. 97-105 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takeshi Isoyama: "Lower concentration of La protein required for internal ribosome entry on hepatitis C virus RNA than poliovirus RNA"J. Gen. Virol.. 80(9). 2319-2327 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Qingmei Jia: "Isolation and molecular characterization of a poliovirus type 1 mutant that replicates in the spinal cord of mice"J. Virol.. 73(7). 6041-6047 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shusuke Kuge: "Redox Regulation of Cell Signaling and Its Clinical Application"Marcel Dekker,Inc.. 328 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiko Ishii: "A new internal ribosomal entry site 5′ boundary is required for poliovirus translation initiation in a mouse system" J.Virol.72(3). 2398-2405 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Minetaro Arita: "Interaction of poliovirus with its purified receptor and conformational alteration in the virion" J.Virol.72(5). 3578-3586 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.-L.Oh: "Determination of functional domains in polypyrimidine-tract-binding protein" Biochem.J.331(1). 169-175 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shusuke Kuge: "Crm 1(Xpo 1) dependent nuclear export of the budding yeast transcription factor yAP-1 is sensitive to oxidative stress" Genes to Cells. 3(8). 521-532 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Seii Ohka: "Retrograde transport of intact poliovirus through the axon via the fast transport system" Virolog. 250(1). 67-75 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Minetaro Arita: "Interaction of poliovirus with its receptor affords a high level of infectivity to the virion in poliovirus infections mediated by the Fc receptor" J.Virol.73(2). 1066-1074 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Akio Nomoto: "Encyclopedia of Molecular Biology" John Wiley & Sons (in press), (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kazuko Shiroki: "Host range phenotype induced by mutations in the internal ribosomal entry site of poliovirus RNA" J.Virol. 71(1). 1-8 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shusuke Kuge: "Regulation of yAP-1 nuclear localization in response to oxidative stress" EMBOJ. 16(7). 1710-1720 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wei-Xing Yang: "Efficient delivery of circulating poliovirus to the central nervous system independently of polioviurs receptor" Virology. 229(2). 421-428 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nobuhiko Kamoshita: "Genetic analysis of intermal ribosomal entry site on hepatitis C virus RNA" Virology. 223(1). 9-18 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Katsuya Tsuchihara: "Specific interaction of polypyrimidine tract-binding protein with the extreme 3` termianl structure of the hepatitis C virus genome,the3`x" J.Virology. 71(9). 6720-6726. (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Junken Aoki: "Mouse homolog of poliovirus receptor-related gene 2 product,mPRR2,mediates cell aggregation" Exp.Cell Res.235. 374-384 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] David Wood: "Transgenic mice:a new model for poliovirus vaccine testing" Scand.J.Lab.Anim.Sci.23(1). 263-267 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kazuko Shiroki: "Host range phenotype induced by mutations in the internal ribosomal entry site of poliovirus RNA" J.Virol.71(1). 1-8 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shusuke Kuge: "Regulation of yAP-1 nuclear localization in response to oxidative stress" EMBO J.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Wei-Xing Yang: "Efficient delivery of circulating poliovirus to the central nervous system independently of poliovirus receptor" Virology. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Junken Aoki: "Mouse homolog of poliovirus receptor-related gene 2,mPRR2,mediates cell aggregation" Exp.Cell Res.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] P.Pognonec: "Cross-family interaction between the bHLHZip USF and bZip Fral proteins results in down-regulation of AP1 activity" Oncogene. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akio Nomoto: "Microbe Hunters:Then and Now" Medi-Ed Press,Bloomington,Illinois, 456 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akio Nomoto: "Hepatitis C virus and its involvement in the development of hepatocellular carcinoma" Princeton Scientific Publishing Co.,Inc.,New Jersey, 282 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shinobu Abe: "Neurobirulence test for oral live poliovaccines using poliovirus-sensitive transgenic mice" Virology. 206. 1075-1083 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Bernard P.Mahon: "Poliovirus-specific CD4_+Th1 clones with both cytotoxic and helper activity mediate protective humoral immunity against a lethal poliovirus infection..." J.Exp.Med.181. 1285-1292 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shinobu Abe: "Studies on neurovirulence in poliovirues sensitive-transgenic mice and cynomolgus monkeys for the different..." Virology. 210. 160-166 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazuko Shiroki: "A new cis-acting element for RNA replication within the 5′ noncoding region of poliovirus type 1 RNA" J.Virol.69. 6825-6832 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Akiko Nomoto: "Recombinant polioviruses as candidate strains of oral poliovaccines" Dev.Biol.Stand.84. 123-127 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Junken Aoki: "Mouse homolog of poliovirus receptor mediates homophilic cell aggregation" J.Biol.Chem.in press.

    • Related Report
      1995 Annual Research Report
  • [Publications] Akiko Nomoto: "The World Congress on Alternatives and Animal Use in the Life" Alan M.Goldberg and L.F.M.van Zutphen,Mary Ann Liebert,Inc., 738 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Akiko Nomoto: "Hepatitis C virus and its involvement in the development of hepatocellular carcinoma" The Princess Takamatsu Cancer Research Fund(in press),

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2018-03-28  

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